IMMUNNOMORPHOLOGICAL INDICATORS OF BLOOD SERUM IN THE CANIDAE FAMILY AFTER MELACRYL IMPLANTATION

• Published in: Siberian Journal of Life Sciences and Agriculture
• Main Authors: Iraida I. Okulova, Julia A. Berezina, Zinaida N. Beltyukova, Igor A. Domskij, Oleg Yu. Bespyatykh
• Format: Article in Journal/Newspaper
• Language: English; Russian
• Published: Science and Innovation Center Publishing House 2021
• Subjects: lysozyme activity of blood serum; opsonophagocytic reaction; protein fractions; alkaline phosphatase; alanine aminotransferase; aspartate aminotransferase; Agriculture; S; Science; Q; Arctic; Arctic Fox; polar fox
• Online Access: https://doi.org/10.12731/2658-6649-2021-13-5-11-25; https://doaj.org/article/859c3d8e191d4b3c90cf9c91c77858fb

Purpose. Investigate the effect of melacryl on biochemical and immunological parameters of red fox (Vulpes vulpes L.) and polar fox (V. Lagopus L.). Materials and methods. For the research the medication Melacryl was used that was developed by the employees of AO “Inpolimed” in cooperation with the Research Institute of fur farming and rabbit breeding them. V.A. Afanasyev (USSR author’s certificate No. 1579489). For the experiment animals were taken from the breeding farm OOO “Vyatka”, Slobodskoy district, Kirov region. The animals are bred in captivity to obtain valuable winter fur. 4 groups were formed for carring out of the experiment: experimental and control group of red foxes (Vulpes vulpes L.) and experimental and control of arctic fox (V. Lagopus L.), in each group there were (n=10 animals). Melacryl (produced by the Kazan Biofactory) was implanted in June according to the instructions for use of the preparation – 10 mg per head into the skin fold of the neck by using a special syringe – implantagor. Laboratory studies were carried out in the laboratory of the FBGNU VNIIOZ them. prof. B.M. Zhitkov. Research methods. Protein fractions in the blood serum were identified according to the nephelometric method of V.Ya. Antonov et al., lysozyme activity of blood serum were identified according to the method of VG Dorofeychuk. The activity of alkaline phosphatase, alanine aminotransferase (ALT), aspartate aminotransferase (ASAT), glucose, total blood serum protein were defined with a semiautomatic biochemical analyzer “Biochim SA” (USA, 2019) by using reagent kits from High Technology (USA, 2019). Bactericidal activity of blood serum were defined according to the method of T.A. Kuzmina. the opsonization was carried out according to the method to A.S. Labinskaya. The Streeter’s numerical index was used to assessment the opsonization reaction. The obtained digital data were processed on an IBM personal computer by using the statistical software package “Statgraphics” and “HG”. The assessment of the reliability of ...