Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue
Cushing’s syndrome, or hypercortisolism (HC), a common endocrinopathy in adult dogs, is caused by chronic hypercortisolemia. Among different metabolic disorders, this syndrome is associated with enhanced subcutaneous lipolysis and visceral adiposity. However, effects of HC in adipose tissue, especia...
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ftdoajarticles:oai:doaj.org/article:8558f749fb61429f94c12e4ccbf95779 2023-05-15T15:50:35+02:00 Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl 2022-05-01T00:00:00Z https://doi.org/10.3390/vetsci9060250 https://doaj.org/article/8558f749fb61429f94c12e4ccbf95779 EN eng MDPI AG https://www.mdpi.com/2306-7381/9/6/250 https://doaj.org/toc/2306-7381 doi:10.3390/vetsci9060250 2306-7381 https://doaj.org/article/8558f749fb61429f94c12e4ccbf95779 Veterinary Sciences, Vol 9, Iss 250, p 250 (2022) dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model Veterinary medicine SF600-1100 article 2022 ftdoajarticles https://doi.org/10.3390/vetsci9060250 2022-12-30T21:30:55Z Cushing’s syndrome, or hypercortisolism (HC), a common endocrinopathy in adult dogs, is caused by chronic hypercortisolemia. Among different metabolic disorders, this syndrome is associated with enhanced subcutaneous lipolysis and visceral adiposity. However, effects of HC in adipose tissue, especially regarding visceral adipose tissue (VAT), are still poorly understood. Herein, the transcriptomic effects of chronic HC on VAT of dogs were evaluated. For this, subcutaneously implanted ACTH-releasing pumps were used, followed by deep RNA sequencing of the canine VAT. Prolonged HC seems to affect a plethora of regulatory mechanisms in VAT of treated dogs, with 1190 differentially expressed genes (DEGs, p and FDR < 0.01) being found. The 691 downregulated DEGs were mostly associated with functional terms like cell adhesion and migration, intracellular signaling, immune response, extracellular matrix and angiogenesis. Treatment also appeared to modulate local glucocorticoid and insulin signaling and hormonal sensitivity, and several factors, e.g., TIMP4, FGF1, CCR2, CXCR4 and HSD11B1/2, were identified as possible important players in the glucocorticoid-related expansion of VAT. Modulation of their function during chronic HC might present interesting targets for further clinical studies. Similarities in the effects of chronic HC on VAT of dogs and humans are highlighted. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Veterinary Sciences 9 6 250 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model Veterinary medicine SF600-1100 |
spellingShingle |
dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model Veterinary medicine SF600-1100 Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
topic_facet |
dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model Veterinary medicine SF600-1100 |
description |
Cushing’s syndrome, or hypercortisolism (HC), a common endocrinopathy in adult dogs, is caused by chronic hypercortisolemia. Among different metabolic disorders, this syndrome is associated with enhanced subcutaneous lipolysis and visceral adiposity. However, effects of HC in adipose tissue, especially regarding visceral adipose tissue (VAT), are still poorly understood. Herein, the transcriptomic effects of chronic HC on VAT of dogs were evaluated. For this, subcutaneously implanted ACTH-releasing pumps were used, followed by deep RNA sequencing of the canine VAT. Prolonged HC seems to affect a plethora of regulatory mechanisms in VAT of treated dogs, with 1190 differentially expressed genes (DEGs, p and FDR < 0.01) being found. The 691 downregulated DEGs were mostly associated with functional terms like cell adhesion and migration, intracellular signaling, immune response, extracellular matrix and angiogenesis. Treatment also appeared to modulate local glucocorticoid and insulin signaling and hormonal sensitivity, and several factors, e.g., TIMP4, FGF1, CCR2, CXCR4 and HSD11B1/2, were identified as possible important players in the glucocorticoid-related expansion of VAT. Modulation of their function during chronic HC might present interesting targets for further clinical studies. Similarities in the effects of chronic HC on VAT of dogs and humans are highlighted. |
format |
Article in Journal/Newspaper |
author |
Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl |
author_facet |
Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl |
author_sort |
Miguel Tavares Pereira |
title |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_short |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_full |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_fullStr |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_full_unstemmed |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_sort |
effects of acth-induced long-term hypercortisolism on the transcriptome of canine visceral adipose tissue |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/vetsci9060250 https://doaj.org/article/8558f749fb61429f94c12e4ccbf95779 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Veterinary Sciences, Vol 9, Iss 250, p 250 (2022) |
op_relation |
https://www.mdpi.com/2306-7381/9/6/250 https://doaj.org/toc/2306-7381 doi:10.3390/vetsci9060250 2306-7381 https://doaj.org/article/8558f749fb61429f94c12e4ccbf95779 |
op_doi |
https://doi.org/10.3390/vetsci9060250 |
container_title |
Veterinary Sciences |
container_volume |
9 |
container_issue |
6 |
container_start_page |
250 |
_version_ |
1766385567694585856 |