A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.

Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito...

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Published in:PLOS Neglected Tropical Diseases
Main Authors: Serena Y Zhao, Grant L Hughes, Kerri L Coon
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2023
Subjects:
Online Access:https://doi.org/10.1371/journal.pntd.0011234
https://doaj.org/article/854b2fbb809544bab5361c28e89feb35
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spelling ftdoajarticles:oai:doaj.org/article:854b2fbb809544bab5361c28e89feb35 2023-06-11T04:09:57+02:00 A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. Serena Y Zhao Grant L Hughes Kerri L Coon 2023-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011234 https://doaj.org/article/854b2fbb809544bab5361c28e89feb35 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011234 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011234 https://doaj.org/article/854b2fbb809544bab5361c28e89feb35 PLoS Neglected Tropical Diseases, Vol 17, Iss 4, p e0011234 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011234 2023-05-07T00:31:57Z Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito studies control for host genotype and environmental conditions, the impact of microbiota variation on phenotypic outcomes of mosquitoes is often unaccounted for. The inability to conduct reproducible intra- and inter-laboratory studies of mosquito-microbiota interactions has also greatly limited our ability to identify microbial targets for mosquito-borne disease control. Here, we developed an approach to isolate and cryopreserve bacterial communities derived from lab and field-based larval rearing environments of the yellow fever mosquito Aedes aegypti-a primary vector of dengue, Zika, and chikungunya viruses. We then validated the use of our approach to generate experimental microcosms colonized by standardized lab- and field-derived bacterial communities. Our results overall reveal minimal effects of cryopreservation on the recovery of both lab- and field-derived bacteria when directly compared with isolation from non-cryopreserved fresh material. Our results also reveal improved reproducibility of bacterial communities in replicate microcosms generated using cryopreserved stocks over fresh material. Communities in replicate microcosms further captured the majority of total bacterial diversity present in both lab- and field-based larval environments, although the relative richness of recovered taxa as compared to non-recovered taxa was substantially lower in microcosms containing field-derived bacteria. Altogether, these results provide a critical next step toward the standardization of mosquito studies to include larval rearing environments colonized by defined microbial communities. They also lay the foundation for long-term studies of mosquito-microbe interactions and the identification and manipulation of taxa ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 4 e0011234
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Serena Y Zhao
Grant L Hughes
Kerri L Coon
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
topic_facet Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
description Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito studies control for host genotype and environmental conditions, the impact of microbiota variation on phenotypic outcomes of mosquitoes is often unaccounted for. The inability to conduct reproducible intra- and inter-laboratory studies of mosquito-microbiota interactions has also greatly limited our ability to identify microbial targets for mosquito-borne disease control. Here, we developed an approach to isolate and cryopreserve bacterial communities derived from lab and field-based larval rearing environments of the yellow fever mosquito Aedes aegypti-a primary vector of dengue, Zika, and chikungunya viruses. We then validated the use of our approach to generate experimental microcosms colonized by standardized lab- and field-derived bacterial communities. Our results overall reveal minimal effects of cryopreservation on the recovery of both lab- and field-derived bacteria when directly compared with isolation from non-cryopreserved fresh material. Our results also reveal improved reproducibility of bacterial communities in replicate microcosms generated using cryopreserved stocks over fresh material. Communities in replicate microcosms further captured the majority of total bacterial diversity present in both lab- and field-based larval environments, although the relative richness of recovered taxa as compared to non-recovered taxa was substantially lower in microcosms containing field-derived bacteria. Altogether, these results provide a critical next step toward the standardization of mosquito studies to include larval rearing environments colonized by defined microbial communities. They also lay the foundation for long-term studies of mosquito-microbe interactions and the identification and manipulation of taxa ...
format Article in Journal/Newspaper
author Serena Y Zhao
Grant L Hughes
Kerri L Coon
author_facet Serena Y Zhao
Grant L Hughes
Kerri L Coon
author_sort Serena Y Zhao
title A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
title_short A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
title_full A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
title_fullStr A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
title_full_unstemmed A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
title_sort cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
publisher Public Library of Science (PLoS)
publishDate 2023
url https://doi.org/10.1371/journal.pntd.0011234
https://doaj.org/article/854b2fbb809544bab5361c28e89feb35
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source PLoS Neglected Tropical Diseases, Vol 17, Iss 4, p e0011234 (2023)
op_relation https://doi.org/10.1371/journal.pntd.0011234
https://doaj.org/toc/1935-2727
https://doaj.org/toc/1935-2735
1935-2727
1935-2735
doi:10.1371/journal.pntd.0011234
https://doaj.org/article/854b2fbb809544bab5361c28e89feb35
op_doi https://doi.org/10.1371/journal.pntd.0011234
container_title PLOS Neglected Tropical Diseases
container_volume 17
container_issue 4
container_start_page e0011234
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