A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats.
Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito...
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ftdoajarticles:oai:doaj.org/article:854b2fbb809544bab5361c28e89feb35 2023-06-11T04:09:57+02:00 A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. Serena Y Zhao Grant L Hughes Kerri L Coon 2023-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011234 https://doaj.org/article/854b2fbb809544bab5361c28e89feb35 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011234 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011234 https://doaj.org/article/854b2fbb809544bab5361c28e89feb35 PLoS Neglected Tropical Diseases, Vol 17, Iss 4, p e0011234 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011234 2023-05-07T00:31:57Z Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito studies control for host genotype and environmental conditions, the impact of microbiota variation on phenotypic outcomes of mosquitoes is often unaccounted for. The inability to conduct reproducible intra- and inter-laboratory studies of mosquito-microbiota interactions has also greatly limited our ability to identify microbial targets for mosquito-borne disease control. Here, we developed an approach to isolate and cryopreserve bacterial communities derived from lab and field-based larval rearing environments of the yellow fever mosquito Aedes aegypti-a primary vector of dengue, Zika, and chikungunya viruses. We then validated the use of our approach to generate experimental microcosms colonized by standardized lab- and field-derived bacterial communities. Our results overall reveal minimal effects of cryopreservation on the recovery of both lab- and field-derived bacteria when directly compared with isolation from non-cryopreserved fresh material. Our results also reveal improved reproducibility of bacterial communities in replicate microcosms generated using cryopreserved stocks over fresh material. Communities in replicate microcosms further captured the majority of total bacterial diversity present in both lab- and field-based larval environments, although the relative richness of recovered taxa as compared to non-recovered taxa was substantially lower in microcosms containing field-derived bacteria. Altogether, these results provide a critical next step toward the standardization of mosquito studies to include larval rearing environments colonized by defined microbial communities. They also lay the foundation for long-term studies of mosquito-microbe interactions and the identification and manipulation of taxa ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 4 e0011234 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Serena Y Zhao Grant L Hughes Kerri L Coon A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito studies control for host genotype and environmental conditions, the impact of microbiota variation on phenotypic outcomes of mosquitoes is often unaccounted for. The inability to conduct reproducible intra- and inter-laboratory studies of mosquito-microbiota interactions has also greatly limited our ability to identify microbial targets for mosquito-borne disease control. Here, we developed an approach to isolate and cryopreserve bacterial communities derived from lab and field-based larval rearing environments of the yellow fever mosquito Aedes aegypti-a primary vector of dengue, Zika, and chikungunya viruses. We then validated the use of our approach to generate experimental microcosms colonized by standardized lab- and field-derived bacterial communities. Our results overall reveal minimal effects of cryopreservation on the recovery of both lab- and field-derived bacteria when directly compared with isolation from non-cryopreserved fresh material. Our results also reveal improved reproducibility of bacterial communities in replicate microcosms generated using cryopreserved stocks over fresh material. Communities in replicate microcosms further captured the majority of total bacterial diversity present in both lab- and field-based larval environments, although the relative richness of recovered taxa as compared to non-recovered taxa was substantially lower in microcosms containing field-derived bacteria. Altogether, these results provide a critical next step toward the standardization of mosquito studies to include larval rearing environments colonized by defined microbial communities. They also lay the foundation for long-term studies of mosquito-microbe interactions and the identification and manipulation of taxa ... |
format |
Article in Journal/Newspaper |
author |
Serena Y Zhao Grant L Hughes Kerri L Coon |
author_facet |
Serena Y Zhao Grant L Hughes Kerri L Coon |
author_sort |
Serena Y Zhao |
title |
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
title_short |
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
title_full |
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
title_fullStr |
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
title_full_unstemmed |
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
title_sort |
cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011234 https://doaj.org/article/854b2fbb809544bab5361c28e89feb35 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 4, p e0011234 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011234 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011234 https://doaj.org/article/854b2fbb809544bab5361c28e89feb35 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011234 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
4 |
container_start_page |
e0011234 |
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1768383984876650496 |