Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas
ABSTRACT For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacte...
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American Society for Microbiology
2022
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ftdoajarticles:oai:doaj.org/article:853ebabad0a742dbbae640e3dcecaced 2023-05-15T15:57:54+02:00 Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas Lizenn Delisle Olivier Laroche Zoë Hilton Jean-François Burguin Anne Rolton Jolene Berry Xavier Pochon Pierre Boudry Julien Vignier 2022-12-01T00:00:00Z https://doi.org/10.1128/spectrum.01959-22 https://doaj.org/article/853ebabad0a742dbbae640e3dcecaced EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/spectrum.01959-22 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.01959-22 2165-0497 https://doaj.org/article/853ebabad0a742dbbae640e3dcecaced Microbiology Spectrum, Vol 10, Iss 6 (2022) OsHV-1 Pacific oyster POMS microbiome 16S rRNA gene sequencing droplet digital PCR Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.1128/spectrum.01959-22 2022-12-30T19:33:33Z ABSTRACT For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacteria in affected oysters. However, the role of the oyster microbiota in POMS is not fully understood. While some bacteria can protect hosts from infection, even minor changes to the microbial communities may also facilitate infection and worsen disease severity. Using a laboratory-based experimental infection model, we challenged juveniles from 10 biparental oyster families with previously established contrasted genetically based ability to survive POMS in the field. Combining molecular analyses and 16S rRNA gene sequencing with histopathological observations, we described the temporal kinetics of POMS and characterized the changes in microbiota during infection. By associating the microbiota composition with oyster mortality rate, viral load, and viral gene expression, we were able to identify both potentially harmful and beneficial bacterial amplicon sequence variants (ASVs). We also observed a delay in viral infection resulting in a later onset of mortality in oysters compared to previous observations and a lack of evidence of fatal dysbiosis in infected oysters. Overall, these results provide new insights into how the oyster microbiome may influence POMS disease outcomes and open new perspectives on the use of microbiome composition as a complementary screening tool to determine shellfish health and potentially predict oyster vulnerability to POMS. IMPORTANCE For more than a decade, Pacific oyster mortality syndrome (POMS) has severely impacted the Crassostrea gigas aquaculture industry, at times killing up to 100% of young farmed Pacific oysters, a key commercial species that is cultivated globally. These disease outbreaks have caused major financial losses for the oyster aquaculture industry. Selective breeding ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Microbiology Spectrum 10 6 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
OsHV-1 Pacific oyster POMS microbiome 16S rRNA gene sequencing droplet digital PCR Microbiology QR1-502 |
spellingShingle |
OsHV-1 Pacific oyster POMS microbiome 16S rRNA gene sequencing droplet digital PCR Microbiology QR1-502 Lizenn Delisle Olivier Laroche Zoë Hilton Jean-François Burguin Anne Rolton Jolene Berry Xavier Pochon Pierre Boudry Julien Vignier Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
topic_facet |
OsHV-1 Pacific oyster POMS microbiome 16S rRNA gene sequencing droplet digital PCR Microbiology QR1-502 |
description |
ABSTRACT For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacteria in affected oysters. However, the role of the oyster microbiota in POMS is not fully understood. While some bacteria can protect hosts from infection, even minor changes to the microbial communities may also facilitate infection and worsen disease severity. Using a laboratory-based experimental infection model, we challenged juveniles from 10 biparental oyster families with previously established contrasted genetically based ability to survive POMS in the field. Combining molecular analyses and 16S rRNA gene sequencing with histopathological observations, we described the temporal kinetics of POMS and characterized the changes in microbiota during infection. By associating the microbiota composition with oyster mortality rate, viral load, and viral gene expression, we were able to identify both potentially harmful and beneficial bacterial amplicon sequence variants (ASVs). We also observed a delay in viral infection resulting in a later onset of mortality in oysters compared to previous observations and a lack of evidence of fatal dysbiosis in infected oysters. Overall, these results provide new insights into how the oyster microbiome may influence POMS disease outcomes and open new perspectives on the use of microbiome composition as a complementary screening tool to determine shellfish health and potentially predict oyster vulnerability to POMS. IMPORTANCE For more than a decade, Pacific oyster mortality syndrome (POMS) has severely impacted the Crassostrea gigas aquaculture industry, at times killing up to 100% of young farmed Pacific oysters, a key commercial species that is cultivated globally. These disease outbreaks have caused major financial losses for the oyster aquaculture industry. Selective breeding ... |
format |
Article in Journal/Newspaper |
author |
Lizenn Delisle Olivier Laroche Zoë Hilton Jean-François Burguin Anne Rolton Jolene Berry Xavier Pochon Pierre Boudry Julien Vignier |
author_facet |
Lizenn Delisle Olivier Laroche Zoë Hilton Jean-François Burguin Anne Rolton Jolene Berry Xavier Pochon Pierre Boudry Julien Vignier |
author_sort |
Lizenn Delisle |
title |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_short |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_full |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_fullStr |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_full_unstemmed |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_sort |
understanding the dynamic of poms infection and the role of microbiota composition in the survival of pacific oysters, crassostrea gigas |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
https://doi.org/10.1128/spectrum.01959-22 https://doaj.org/article/853ebabad0a742dbbae640e3dcecaced |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Microbiology Spectrum, Vol 10, Iss 6 (2022) |
op_relation |
https://journals.asm.org/doi/10.1128/spectrum.01959-22 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.01959-22 2165-0497 https://doaj.org/article/853ebabad0a742dbbae640e3dcecaced |
op_doi |
https://doi.org/10.1128/spectrum.01959-22 |
container_title |
Microbiology Spectrum |
container_volume |
10 |
container_issue |
6 |
_version_ |
1766393613713932288 |