Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro
ABSTRACT Background: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potent...
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ftdoajarticles:oai:doaj.org/article:853b1e5400b246b386c505a81a843446 2023-05-15T15:11:58+02:00 Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro Jessica Ohana Lemes Carneiro-Goetten Bruna Santos Rodrigues Rodrigo Amauri Nogoceke Thatyanne Gradowski do Nascimento Andrea Novais Moreno-Amaral Patricia Maria Stuelp-Campelo Selene Elifio-Esposito https://doi.org/10.1590/1678-9199-jvatitd-2019-0073 https://doaj.org/article/853b1e5400b246b386c505a81a843446 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100802&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0073 https://doaj.org/article/853b1e5400b246b386c505a81a843446 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 snake venom neuroblastoma sk-n-sh cells c-type lectin polymorphonuclear leukocyte neoplasm invasion Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0073 2022-12-31T11:55:23Z ABSTRACT Background: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potentialize the immunotherapy, a suggested approach for high-risk patients. Preview studies showed the antitumoral potential of BJcuL, a C- type lectin isolated from Bothrops jararacussu venom. It was able to induce immunomodulatory responses, promoting the rolling and adhesion of leukocytes and the activation of neutrophils. Methods: SK-N-SH cells were incubated with conditioned media (CM) obtained during the treatment of neutrophils with BJcuL and fMLP, a bacteria-derived peptide highly effective for activating neutrophil functions. Then we evaluated the effect of the same stimulation on the co-cultivation of neutrophils and SK-N-SH cells. Tumor cells were tested for viability, migration, and invasion potential. Results: In the viability assay, only neutrophils treated with BJcuL (24 h) and cultivated with SK-N-SH were cytotoxic. Migration of tumor cells decreased when incubated directly (p < 0.001) or indirectly (p < 0.005) with untreated neutrophils. When invasion potential was evaluated, neutrophils incubated with BJcuL reduced the total number of colonies of SK-N-SH cells following co-cultivation for 24 h (p < 0.005). Treatment with CM resulted in decreased anchorage-free survival following 24 h of treatment (p < 0.001). Conclusion: Data demonstrated that SK-N-SH cells maintain their migratory potential in the face of neutrophil modulation by BJcuL, but their invasive capacity was significantly reduced. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Anchorage Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
snake venom neuroblastoma sk-n-sh cells c-type lectin polymorphonuclear leukocyte neoplasm invasion Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
snake venom neuroblastoma sk-n-sh cells c-type lectin polymorphonuclear leukocyte neoplasm invasion Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Jessica Ohana Lemes Carneiro-Goetten Bruna Santos Rodrigues Rodrigo Amauri Nogoceke Thatyanne Gradowski do Nascimento Andrea Novais Moreno-Amaral Patricia Maria Stuelp-Campelo Selene Elifio-Esposito Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro |
topic_facet |
snake venom neuroblastoma sk-n-sh cells c-type lectin polymorphonuclear leukocyte neoplasm invasion Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
ABSTRACT Background: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potentialize the immunotherapy, a suggested approach for high-risk patients. Preview studies showed the antitumoral potential of BJcuL, a C- type lectin isolated from Bothrops jararacussu venom. It was able to induce immunomodulatory responses, promoting the rolling and adhesion of leukocytes and the activation of neutrophils. Methods: SK-N-SH cells were incubated with conditioned media (CM) obtained during the treatment of neutrophils with BJcuL and fMLP, a bacteria-derived peptide highly effective for activating neutrophil functions. Then we evaluated the effect of the same stimulation on the co-cultivation of neutrophils and SK-N-SH cells. Tumor cells were tested for viability, migration, and invasion potential. Results: In the viability assay, only neutrophils treated with BJcuL (24 h) and cultivated with SK-N-SH were cytotoxic. Migration of tumor cells decreased when incubated directly (p < 0.001) or indirectly (p < 0.005) with untreated neutrophils. When invasion potential was evaluated, neutrophils incubated with BJcuL reduced the total number of colonies of SK-N-SH cells following co-cultivation for 24 h (p < 0.005). Treatment with CM resulted in decreased anchorage-free survival following 24 h of treatment (p < 0.001). Conclusion: Data demonstrated that SK-N-SH cells maintain their migratory potential in the face of neutrophil modulation by BJcuL, but their invasive capacity was significantly reduced. |
format |
Article in Journal/Newspaper |
author |
Jessica Ohana Lemes Carneiro-Goetten Bruna Santos Rodrigues Rodrigo Amauri Nogoceke Thatyanne Gradowski do Nascimento Andrea Novais Moreno-Amaral Patricia Maria Stuelp-Campelo Selene Elifio-Esposito |
author_facet |
Jessica Ohana Lemes Carneiro-Goetten Bruna Santos Rodrigues Rodrigo Amauri Nogoceke Thatyanne Gradowski do Nascimento Andrea Novais Moreno-Amaral Patricia Maria Stuelp-Campelo Selene Elifio-Esposito |
author_sort |
Jessica Ohana Lemes Carneiro-Goetten |
title |
Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro |
title_short |
Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro |
title_full |
Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro |
title_fullStr |
Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro |
title_full_unstemmed |
Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro |
title_sort |
neutrophils activated by bjcul, a c-type lectin isolated from bothrops jararacussu venom, decrease the invasion potential of neuroblastoma sk-n-sh cells in vitro |
publisher |
SciELO |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0073 https://doaj.org/article/853b1e5400b246b386c505a81a843446 |
geographic |
Arctic Anchorage |
geographic_facet |
Arctic Anchorage |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100802&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0073 https://doaj.org/article/853b1e5400b246b386c505a81a843446 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0073 |
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Journal of Venomous Animals and Toxins including Tropical Diseases |
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26 |
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