Chlorella mirabilis as a potential species for biomass production in low temperature environment
Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabi...
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ftdoajarticles:oai:doaj.org/article:8539891b93d74608ac865e48fae29db6 2023-05-15T13:44:31+02:00 Chlorella mirabilis as a potential species for biomass production in low temperature environment Satya Prakash Shukla Jana eKviderova Jan eTříska Josef eElster 2013-04-01T00:00:00Z https://doi.org/10.3389/fmicb.2013.00097 https://doaj.org/article/8539891b93d74608ac865e48fae29db6 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00097/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2013.00097 https://doaj.org/article/8539891b93d74608ac865e48fae29db6 Frontiers in Microbiology, Vol 4 (2013) Microalgae growth rate Low temperature N and C manipulation fatty acid content Microbiology QR1-502 article 2013 ftdoajarticles https://doi.org/10.3389/fmicb.2013.00097 2022-12-31T10:02:55Z Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. The micro-scale mass cultivation was performed in stable (in-door) and variable (out-door) conditions during winter and/or early spring in the Czech Republic. In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z), Z medium + 5% glycerol (ZG), Z medium + 5% glycerol + 50 µM KNO3 (ZGN), Z medium + 5% glycerol + 200µM NH4Cl (ZGA), Z medium + 5% glycerol + 1 mM Na2CO3 (ZNC), Z medium + 5% glycerol + 1 mM Na2CO3 + 200µM NH4Cl (ZGCA) and Z medium + 5% glycerol + 1 mM Na2CO3 + 50 µM KNO3 (ZGCN) and were performed at 15°C with an irradiance of 75 µmol m-2 s-1. During the out-door experiments, the night-day temperature ranged from -6.6°C to 17.5°C (daily average 3.1±5.3 °C) and irradiance ranged from 0 to 2300 µmol m-2 s-1 (daily average 1500±1090 µmol m-2 s-1). Only the Z, ZG, ZGN and ZGC treatments were used in the out-door cultivation. In the in-door mass cultivation, all nitrogen and carbon sources additions increased the growth rate with the exception of ZGA. When individual sources were considered, only the effect of 5% glycerol addition was significant. On the other hand, the growth rate decreased in the ZG and ZGN treatments in the out-door experiment, probably due to carbon limitation. Fatty acid composition showed increased production of linoleic acid in the glycerol treatments. The studied strain of C. mirabilis is proposed to be a promising source of linoleic acid in low temperature mass cultivation biotechnology. This strain is a perspective model organism for biotechnology in low-temperature conditions. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Frontiers in Microbiology 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Microalgae growth rate Low temperature N and C manipulation fatty acid content Microbiology QR1-502 |
spellingShingle |
Microalgae growth rate Low temperature N and C manipulation fatty acid content Microbiology QR1-502 Satya Prakash Shukla Jana eKviderova Jan eTříska Josef eElster Chlorella mirabilis as a potential species for biomass production in low temperature environment |
topic_facet |
Microalgae growth rate Low temperature N and C manipulation fatty acid content Microbiology QR1-502 |
description |
Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. The micro-scale mass cultivation was performed in stable (in-door) and variable (out-door) conditions during winter and/or early spring in the Czech Republic. In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z), Z medium + 5% glycerol (ZG), Z medium + 5% glycerol + 50 µM KNO3 (ZGN), Z medium + 5% glycerol + 200µM NH4Cl (ZGA), Z medium + 5% glycerol + 1 mM Na2CO3 (ZNC), Z medium + 5% glycerol + 1 mM Na2CO3 + 200µM NH4Cl (ZGCA) and Z medium + 5% glycerol + 1 mM Na2CO3 + 50 µM KNO3 (ZGCN) and were performed at 15°C with an irradiance of 75 µmol m-2 s-1. During the out-door experiments, the night-day temperature ranged from -6.6°C to 17.5°C (daily average 3.1±5.3 °C) and irradiance ranged from 0 to 2300 µmol m-2 s-1 (daily average 1500±1090 µmol m-2 s-1). Only the Z, ZG, ZGN and ZGC treatments were used in the out-door cultivation. In the in-door mass cultivation, all nitrogen and carbon sources additions increased the growth rate with the exception of ZGA. When individual sources were considered, only the effect of 5% glycerol addition was significant. On the other hand, the growth rate decreased in the ZG and ZGN treatments in the out-door experiment, probably due to carbon limitation. Fatty acid composition showed increased production of linoleic acid in the glycerol treatments. The studied strain of C. mirabilis is proposed to be a promising source of linoleic acid in low temperature mass cultivation biotechnology. This strain is a perspective model organism for biotechnology in low-temperature conditions. |
format |
Article in Journal/Newspaper |
author |
Satya Prakash Shukla Jana eKviderova Jan eTříska Josef eElster |
author_facet |
Satya Prakash Shukla Jana eKviderova Jan eTříska Josef eElster |
author_sort |
Satya Prakash Shukla |
title |
Chlorella mirabilis as a potential species for biomass production in low temperature environment |
title_short |
Chlorella mirabilis as a potential species for biomass production in low temperature environment |
title_full |
Chlorella mirabilis as a potential species for biomass production in low temperature environment |
title_fullStr |
Chlorella mirabilis as a potential species for biomass production in low temperature environment |
title_full_unstemmed |
Chlorella mirabilis as a potential species for biomass production in low temperature environment |
title_sort |
chlorella mirabilis as a potential species for biomass production in low temperature environment |
publisher |
Frontiers Media S.A. |
publishDate |
2013 |
url |
https://doi.org/10.3389/fmicb.2013.00097 https://doaj.org/article/8539891b93d74608ac865e48fae29db6 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Microbiology, Vol 4 (2013) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00097/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2013.00097 https://doaj.org/article/8539891b93d74608ac865e48fae29db6 |
op_doi |
https://doi.org/10.3389/fmicb.2013.00097 |
container_title |
Frontiers in Microbiology |
container_volume |
4 |
_version_ |
1766202774134980608 |