Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice.
Japanese encephalitis virus (JEV) is a pathogen that causes severe vector-borne zoonotic diseases, thereby posing a serious threat to human health. Although JEV is potentially neurotropic, its pathogenesis and distribution in the host have not been fully elucidated. In this study, an infected mouse...
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ftdoajarticles:oai:doaj.org/article:851d18505d9a4834bfd0aebd380837c1 2023-05-15T15:17:13+02:00 Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. Wei Han Mingxing Gao Changqing Xie Jinhua Zhang Zikai Zhao Xueying Hu Wanpo Zhang Xiaoli Liu Shengbo Cao Guofu Cheng Changqin Gu 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008442 https://doaj.org/article/851d18505d9a4834bfd0aebd380837c1 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008442 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008442 https://doaj.org/article/851d18505d9a4834bfd0aebd380837c1 PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0008442 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008442 2022-12-31T04:58:46Z Japanese encephalitis virus (JEV) is a pathogen that causes severe vector-borne zoonotic diseases, thereby posing a serious threat to human health. Although JEV is potentially neurotropic, its pathogenesis and distribution in the host have not been fully elucidated. In this study, an infected mouse model was established using a highly virulent P3 strain of JEV. Immunohistochemistry and in situ hybridization, combined with anatomical imaging of the mouse brain, were used to dynamically localize the virus and construct three-dimensional (3D) images. Consequently, onset of mild clinical signs occurred in some mice at 3.5 d post JEV infection, while most mice displayed typical neurological signs at 6 d post-infection (dpi). Moreover, brain pathology revealed typical changes associated with non-suppurative encephalitis, which lasted up to 8 d. The earliest detection of viral antigen was achieved at 3 dpi in the thalamus and medulla oblongata. At 6 dpi, the positive viral antigen signals were mainly distributed in the cerebral cortex, olfactory area, basal ganglia, thalamus, and brainstem regions in mice. At 8 dpi, the antigen signals gradually decreased, and the localization of JEV tended to concentrate in the cerebrum and thalamus, while no viral antigen was detected in the brain at 21 dpi. In this model, the viral antigen was first expressed in the reticular thalamic nucleus (Rt), and the virus content is relatively stable. The expression of the viral antigen in the hippocampal CA2 region, the anterior olfactory nucleus, and the deep mesencephalic nucleus was high and persistent. The 3D images showed that viral signals were mostly concentrated in the parietal cortex, occipital lobe, and hippocampus, near the mid-sagittal plane. In the early stages of infection in mice, a large number of viral antigens were detected in denatured and necrotic neurons, suggesting that JEV directly causes neuronal damage. From the time of its entry, JEV is widely distributed in the central nervous system thereby causing extensive ... Article in Journal/Newspaper Arctic Human health Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0008442 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Wei Han Mingxing Gao Changqing Xie Jinhua Zhang Zikai Zhao Xueying Hu Wanpo Zhang Xiaoli Liu Shengbo Cao Guofu Cheng Changqin Gu Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Japanese encephalitis virus (JEV) is a pathogen that causes severe vector-borne zoonotic diseases, thereby posing a serious threat to human health. Although JEV is potentially neurotropic, its pathogenesis and distribution in the host have not been fully elucidated. In this study, an infected mouse model was established using a highly virulent P3 strain of JEV. Immunohistochemistry and in situ hybridization, combined with anatomical imaging of the mouse brain, were used to dynamically localize the virus and construct three-dimensional (3D) images. Consequently, onset of mild clinical signs occurred in some mice at 3.5 d post JEV infection, while most mice displayed typical neurological signs at 6 d post-infection (dpi). Moreover, brain pathology revealed typical changes associated with non-suppurative encephalitis, which lasted up to 8 d. The earliest detection of viral antigen was achieved at 3 dpi in the thalamus and medulla oblongata. At 6 dpi, the positive viral antigen signals were mainly distributed in the cerebral cortex, olfactory area, basal ganglia, thalamus, and brainstem regions in mice. At 8 dpi, the antigen signals gradually decreased, and the localization of JEV tended to concentrate in the cerebrum and thalamus, while no viral antigen was detected in the brain at 21 dpi. In this model, the viral antigen was first expressed in the reticular thalamic nucleus (Rt), and the virus content is relatively stable. The expression of the viral antigen in the hippocampal CA2 region, the anterior olfactory nucleus, and the deep mesencephalic nucleus was high and persistent. The 3D images showed that viral signals were mostly concentrated in the parietal cortex, occipital lobe, and hippocampus, near the mid-sagittal plane. In the early stages of infection in mice, a large number of viral antigens were detected in denatured and necrotic neurons, suggesting that JEV directly causes neuronal damage. From the time of its entry, JEV is widely distributed in the central nervous system thereby causing extensive ... |
format |
Article in Journal/Newspaper |
author |
Wei Han Mingxing Gao Changqing Xie Jinhua Zhang Zikai Zhao Xueying Hu Wanpo Zhang Xiaoli Liu Shengbo Cao Guofu Cheng Changqin Gu |
author_facet |
Wei Han Mingxing Gao Changqing Xie Jinhua Zhang Zikai Zhao Xueying Hu Wanpo Zhang Xiaoli Liu Shengbo Cao Guofu Cheng Changqin Gu |
author_sort |
Wei Han |
title |
Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. |
title_short |
Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. |
title_full |
Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. |
title_fullStr |
Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. |
title_full_unstemmed |
Precise localization and dynamic distribution of Japanese encephalitis virus in the rain nuclei of infected mice. |
title_sort |
precise localization and dynamic distribution of japanese encephalitis virus in the rain nuclei of infected mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0008442 https://doaj.org/article/851d18505d9a4834bfd0aebd380837c1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Human health |
genre_facet |
Arctic Human health |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0008442 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008442 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008442 https://doaj.org/article/851d18505d9a4834bfd0aebd380837c1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008442 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
6 |
container_start_page |
e0008442 |
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