Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin.
Buruli ulcer (BU), caused by Mycobacterium ulcerans is a chronic necrotizing skin disease. It usually starts with a subcutaneous nodule or plaque containing large clusters of extracellular acid-fast bacilli. Surrounding tissue is destroyed by the cytotoxic macrolide toxin mycolactone produced by mic...
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ftdoajarticles:oai:doaj.org/article:85186e407e4b4f1b9209b286dc94c54e 2023-05-15T15:09:23+02:00 Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. Dorothy Yeboah-Manu Grace S Kpeli Marie-Thérèse Ruf Kobina Asan-Ampah Kwabena Quenin-Fosu Evelyn Owusu-Mireku Albert Paintsil Isaac Lamptey Benjamin Anku Cynthia Kwakye-Maclean Mercy Newman Gerd Pluschke 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002191 https://doaj.org/article/85186e407e4b4f1b9209b286dc94c54e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3642065?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0002191 https://doaj.org/article/85186e407e4b4f1b9209b286dc94c54e PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2191 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002191 2022-12-31T09:48:05Z Buruli ulcer (BU), caused by Mycobacterium ulcerans is a chronic necrotizing skin disease. It usually starts with a subcutaneous nodule or plaque containing large clusters of extracellular acid-fast bacilli. Surrounding tissue is destroyed by the cytotoxic macrolide toxin mycolactone produced by microcolonies of M. ulcerans. Skin covering the destroyed subcutaneous fat and soft tissue may eventually break down leading to the formation of large ulcers that progress, if untreated, over months and years. Here we have analyzed the bacterial flora of BU lesions of three different groups of patients before, during and after daily treatment with streptomycin and rifampicin for eight weeks (SR8) and determined drug resistance of the bacteria isolated from the lesions. Before SR8 treatment, more than 60% of the examined BU lesions were infected with other bacteria, with Staphylococcus aureus and Pseudomonas aeruginosa being the most prominent ones. During treatment, 65% of all lesions were still infected, mainly with P. aeruginosa. After completion of SR8 treatment, still more than 75% of lesions clinically suspected to be infected were microbiologically confirmed as infected, mainly with P. aeruginosa or Proteus miriabilis. Drug susceptibility tests revealed especially for S. aureus a high frequency of resistance to the first line drugs used in Ghana. Our results show that secondary infection of BU lesions is common. This could lead to delayed healing and should therefore be further investigated. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 5 e2191 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Dorothy Yeboah-Manu Grace S Kpeli Marie-Thérèse Ruf Kobina Asan-Ampah Kwabena Quenin-Fosu Evelyn Owusu-Mireku Albert Paintsil Isaac Lamptey Benjamin Anku Cynthia Kwakye-Maclean Mercy Newman Gerd Pluschke Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Buruli ulcer (BU), caused by Mycobacterium ulcerans is a chronic necrotizing skin disease. It usually starts with a subcutaneous nodule or plaque containing large clusters of extracellular acid-fast bacilli. Surrounding tissue is destroyed by the cytotoxic macrolide toxin mycolactone produced by microcolonies of M. ulcerans. Skin covering the destroyed subcutaneous fat and soft tissue may eventually break down leading to the formation of large ulcers that progress, if untreated, over months and years. Here we have analyzed the bacterial flora of BU lesions of three different groups of patients before, during and after daily treatment with streptomycin and rifampicin for eight weeks (SR8) and determined drug resistance of the bacteria isolated from the lesions. Before SR8 treatment, more than 60% of the examined BU lesions were infected with other bacteria, with Staphylococcus aureus and Pseudomonas aeruginosa being the most prominent ones. During treatment, 65% of all lesions were still infected, mainly with P. aeruginosa. After completion of SR8 treatment, still more than 75% of lesions clinically suspected to be infected were microbiologically confirmed as infected, mainly with P. aeruginosa or Proteus miriabilis. Drug susceptibility tests revealed especially for S. aureus a high frequency of resistance to the first line drugs used in Ghana. Our results show that secondary infection of BU lesions is common. This could lead to delayed healing and should therefore be further investigated. |
format |
Article in Journal/Newspaper |
author |
Dorothy Yeboah-Manu Grace S Kpeli Marie-Thérèse Ruf Kobina Asan-Ampah Kwabena Quenin-Fosu Evelyn Owusu-Mireku Albert Paintsil Isaac Lamptey Benjamin Anku Cynthia Kwakye-Maclean Mercy Newman Gerd Pluschke |
author_facet |
Dorothy Yeboah-Manu Grace S Kpeli Marie-Thérèse Ruf Kobina Asan-Ampah Kwabena Quenin-Fosu Evelyn Owusu-Mireku Albert Paintsil Isaac Lamptey Benjamin Anku Cynthia Kwakye-Maclean Mercy Newman Gerd Pluschke |
author_sort |
Dorothy Yeboah-Manu |
title |
Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
title_short |
Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
title_full |
Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
title_fullStr |
Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
title_full_unstemmed |
Secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
title_sort |
secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002191 https://doaj.org/article/85186e407e4b4f1b9209b286dc94c54e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2191 (2013) |
op_relation |
http://europepmc.org/articles/PMC3642065?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0002191 https://doaj.org/article/85186e407e4b4f1b9209b286dc94c54e |
op_doi |
https://doi.org/10.1371/journal.pntd.0002191 |
container_title |
PLoS Neglected Tropical Diseases |
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7 |
container_issue |
5 |
container_start_page |
e2191 |
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1766340597901164544 |