Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries.
BACKGROUND:One root cause of the neglect of rabies is the lack of adequate diagnostic tests in the context of low income countries. A rapid, performance friendly and low cost method to detect rabies virus (RABV) in brain samples will contribute positively to surveillance and consequently to accurate...
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ftdoajarticles:oai:doaj.org/article:84c25075de1c4183832c6eaa39986d55 2023-05-15T15:14:43+02:00 Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. Monique Léchenne Kemdongarti Naïssengar Anthony Lepelletier Idriss Oumar Alfaroukh Hervé Bourhy Jakob Zinsstag Laurent Dacheux 2016-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005010 https://doaj.org/article/84c25075de1c4183832c6eaa39986d55 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5051951?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005010 https://doaj.org/article/84c25075de1c4183832c6eaa39986d55 PLoS Neglected Tropical Diseases, Vol 10, Iss 10, p e0005010 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005010 2022-12-31T15:58:41Z BACKGROUND:One root cause of the neglect of rabies is the lack of adequate diagnostic tests in the context of low income countries. A rapid, performance friendly and low cost method to detect rabies virus (RABV) in brain samples will contribute positively to surveillance and consequently to accurate data reporting, which is presently missing in the majority of rabies endemic countries. METHODOLOGY/PRINCIPAL FINDINGS:We evaluated a rapid immunodiagnostic test (RIDT) in comparison with the standard fluorescent antibody test (FAT) and confirmed the detection of the viral RNA by real time reverse transcription polymerase chain reaction (RT-qPCR). Our analysis is a multicentre approach to validate the performance of the RIDT in both a field laboratory (N'Djamena, Chad) and an international reference laboratory (Institut Pasteur, Paris, France). In the field laboratory, 48 samples from dogs were tested and in the reference laboratory setting, a total of 73 samples was tested, representing a wide diversity of RABV in terms of animal species tested (13 different species), geographical origin of isolates with special emphasis on Africa, and different phylogenetic clades. Under reference laboratory conditions, specificity was 93.3% and sensitivity was 95.3% compared to the gold standard FAT test. Under field laboratory conditions, the RIDT yielded a higher reliability than the FAT test particularly on fresh and decomposed samples. Viral RNA was later extracted directly from the test filter paper and further used successfully for sequencing and genotyping. CONCLUSION/SIGNIFICANCE:The RIDT shows excellent performance qualities both in regard to user friendliness and reliability of the result. In addition, the test cassettes can be used as a vehicle to ship viral RNA to reference laboratories for further laboratory confirmation of the diagnosis and for epidemiological investigations using nucleotide sequencing. The potential for satisfactory use in remote locations is therefore very high to improve the global knowledge of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pasteur ENVELOPE(140.099,140.099,-66.625,-66.625) PLOS Neglected Tropical Diseases 10 10 e0005010 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Monique Léchenne Kemdongarti Naïssengar Anthony Lepelletier Idriss Oumar Alfaroukh Hervé Bourhy Jakob Zinsstag Laurent Dacheux Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:One root cause of the neglect of rabies is the lack of adequate diagnostic tests in the context of low income countries. A rapid, performance friendly and low cost method to detect rabies virus (RABV) in brain samples will contribute positively to surveillance and consequently to accurate data reporting, which is presently missing in the majority of rabies endemic countries. METHODOLOGY/PRINCIPAL FINDINGS:We evaluated a rapid immunodiagnostic test (RIDT) in comparison with the standard fluorescent antibody test (FAT) and confirmed the detection of the viral RNA by real time reverse transcription polymerase chain reaction (RT-qPCR). Our analysis is a multicentre approach to validate the performance of the RIDT in both a field laboratory (N'Djamena, Chad) and an international reference laboratory (Institut Pasteur, Paris, France). In the field laboratory, 48 samples from dogs were tested and in the reference laboratory setting, a total of 73 samples was tested, representing a wide diversity of RABV in terms of animal species tested (13 different species), geographical origin of isolates with special emphasis on Africa, and different phylogenetic clades. Under reference laboratory conditions, specificity was 93.3% and sensitivity was 95.3% compared to the gold standard FAT test. Under field laboratory conditions, the RIDT yielded a higher reliability than the FAT test particularly on fresh and decomposed samples. Viral RNA was later extracted directly from the test filter paper and further used successfully for sequencing and genotyping. CONCLUSION/SIGNIFICANCE:The RIDT shows excellent performance qualities both in regard to user friendliness and reliability of the result. In addition, the test cassettes can be used as a vehicle to ship viral RNA to reference laboratories for further laboratory confirmation of the diagnosis and for epidemiological investigations using nucleotide sequencing. The potential for satisfactory use in remote locations is therefore very high to improve the global knowledge of ... |
format |
Article in Journal/Newspaper |
author |
Monique Léchenne Kemdongarti Naïssengar Anthony Lepelletier Idriss Oumar Alfaroukh Hervé Bourhy Jakob Zinsstag Laurent Dacheux |
author_facet |
Monique Léchenne Kemdongarti Naïssengar Anthony Lepelletier Idriss Oumar Alfaroukh Hervé Bourhy Jakob Zinsstag Laurent Dacheux |
author_sort |
Monique Léchenne |
title |
Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. |
title_short |
Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. |
title_full |
Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. |
title_fullStr |
Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. |
title_full_unstemmed |
Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. |
title_sort |
validation of a rapid rabies diagnostic tool for field surveillance in developing countries. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005010 https://doaj.org/article/84c25075de1c4183832c6eaa39986d55 |
long_lat |
ENVELOPE(140.099,140.099,-66.625,-66.625) |
geographic |
Arctic Pasteur |
geographic_facet |
Arctic Pasteur |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 10, p e0005010 (2016) |
op_relation |
http://europepmc.org/articles/PMC5051951?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005010 https://doaj.org/article/84c25075de1c4183832c6eaa39986d55 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005010 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
10 |
container_start_page |
e0005010 |
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1766345145565839360 |