Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation
Molecular genetic markers of various PCD (programmed cell death) variants during xylo- and phloemogenesis have been identified for the first time in Scots pine under lingonberry pine forest conditions in Northwest Russia (middle taiga subzone). PCD is a genetically determined process. Gene profiles...
Published in: | Plants |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/plants11243438 https://doaj.org/article/848a96211c6e4617861badc78c94b764 |
id |
ftdoajarticles:oai:doaj.org/article:848a96211c6e4617861badc78c94b764 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:848a96211c6e4617861badc78c94b764 2023-05-15T17:46:06+02:00 Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova 2022-12-01T00:00:00Z https://doi.org/10.3390/plants11243438 https://doaj.org/article/848a96211c6e4617861badc78c94b764 EN eng MDPI AG https://www.mdpi.com/2223-7747/11/24/3438 https://doaj.org/toc/2223-7747 doi:10.3390/plants11243438 2223-7747 https://doaj.org/article/848a96211c6e4617861badc78c94b764 Plants, Vol 11, Iss 3438, p 3438 (2022) Pinus sylvestris L programmed cell death xylogenesis heartwood formation bifunctional endonuclease cysteine endopeptidase Botany QK1-989 article 2022 ftdoajarticles https://doi.org/10.3390/plants11243438 2022-12-30T19:30:36Z Molecular genetic markers of various PCD (programmed cell death) variants during xylo- and phloemogenesis have been identified for the first time in Scots pine under lingonberry pine forest conditions in Northwest Russia (middle taiga subzone). PCD is a genetically determined process. Gene profiles of serine and cysteine proteases (endopeptidases), endonucleases, and metacaspases families are often considered markers of the final xylogenesis stage. In the present study, we examined the gene expression profiles of the BFN (bifunctional endonuclease) family— BFN , BFN1 , BFN2 , BFN3 , and peptidase (cysteine endopeptidase, CEP and metacaspase, MC5 ) in the radial row, in addition to the vascular phloem and cambium (F1), differentiating xylem (F2), sapwood (SW), and transition zone during the active cambial growth period of uneven-aged pine trees (25-, 63- and 164-cambial age (c.a.) years old). We have shown that the expression patterns of the PCD-related genes did not depend on the cambial age but were largely determined by plant tissue type. In the radial row F1-F2-SW, we studied the activities of enzymes, including sucrose in metabolism (sucrose synthase, three forms of invertase); antioxidant system (AOS) enzymes (superoxide dismutase, catalase); and peroxidase andpolyphenol oxidase, which belonged to AOS enzymes and were involved in the synthesis of phenolic components of cell walls. The activity of the enzymes indicated that the trunk tissues of pine trees had varying metabolic status. Molecular genetic PCD regulation mechanisms during xylem vascular and mechanical element formation and parenchyma cells’ PCD during the formation of Scots pine heartwood were discussed. Article in Journal/Newspaper Northwest Russia taiga Directory of Open Access Journals: DOAJ Articles Plants 11 24 3438 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pinus sylvestris L programmed cell death xylogenesis heartwood formation bifunctional endonuclease cysteine endopeptidase Botany QK1-989 |
spellingShingle |
Pinus sylvestris L programmed cell death xylogenesis heartwood formation bifunctional endonuclease cysteine endopeptidase Botany QK1-989 Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation |
topic_facet |
Pinus sylvestris L programmed cell death xylogenesis heartwood formation bifunctional endonuclease cysteine endopeptidase Botany QK1-989 |
description |
Molecular genetic markers of various PCD (programmed cell death) variants during xylo- and phloemogenesis have been identified for the first time in Scots pine under lingonberry pine forest conditions in Northwest Russia (middle taiga subzone). PCD is a genetically determined process. Gene profiles of serine and cysteine proteases (endopeptidases), endonucleases, and metacaspases families are often considered markers of the final xylogenesis stage. In the present study, we examined the gene expression profiles of the BFN (bifunctional endonuclease) family— BFN , BFN1 , BFN2 , BFN3 , and peptidase (cysteine endopeptidase, CEP and metacaspase, MC5 ) in the radial row, in addition to the vascular phloem and cambium (F1), differentiating xylem (F2), sapwood (SW), and transition zone during the active cambial growth period of uneven-aged pine trees (25-, 63- and 164-cambial age (c.a.) years old). We have shown that the expression patterns of the PCD-related genes did not depend on the cambial age but were largely determined by plant tissue type. In the radial row F1-F2-SW, we studied the activities of enzymes, including sucrose in metabolism (sucrose synthase, three forms of invertase); antioxidant system (AOS) enzymes (superoxide dismutase, catalase); and peroxidase andpolyphenol oxidase, which belonged to AOS enzymes and were involved in the synthesis of phenolic components of cell walls. The activity of the enzymes indicated that the trunk tissues of pine trees had varying metabolic status. Molecular genetic PCD regulation mechanisms during xylem vascular and mechanical element formation and parenchyma cells’ PCD during the formation of Scots pine heartwood were discussed. |
format |
Article in Journal/Newspaper |
author |
Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova |
author_facet |
Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova |
author_sort |
Yulia L. Moshchenskaya |
title |
Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation |
title_short |
Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation |
title_full |
Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation |
title_fullStr |
Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation |
title_full_unstemmed |
Plant-Programmed Cell Death-Associated Genes Participation in Pinus sylvestris L. Trunk Tissue Formation |
title_sort |
plant-programmed cell death-associated genes participation in pinus sylvestris l. trunk tissue formation |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/plants11243438 https://doaj.org/article/848a96211c6e4617861badc78c94b764 |
genre |
Northwest Russia taiga |
genre_facet |
Northwest Russia taiga |
op_source |
Plants, Vol 11, Iss 3438, p 3438 (2022) |
op_relation |
https://www.mdpi.com/2223-7747/11/24/3438 https://doaj.org/toc/2223-7747 doi:10.3390/plants11243438 2223-7747 https://doaj.org/article/848a96211c6e4617861badc78c94b764 |
op_doi |
https://doi.org/10.3390/plants11243438 |
container_title |
Plants |
container_volume |
11 |
container_issue |
24 |
container_start_page |
3438 |
_version_ |
1766149463771971584 |