A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus.
Chikungunya virus (CHIKV) is an emerging mosquito-transmitted alphavirus that leads to acute fever and chronic debilitating polyarthralgia. To date, the mechanism underlying chronic recurrent arthralgia is unknown. In the present study, newborn wild-type C57BL/6 mice were infected with CHIKV, and th...
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ftdoajarticles:oai:doaj.org/article:84783e9f302f4b038b02f8354901c6c2 2023-05-15T15:11:29+02:00 A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. Yue Zhang Hu Yan Xian Li Dihan Zhou Maohua Zhong Jingyi Yang Bali Zhao Xuxu Fan Jun Fan Jiayi Shu Mengji Lu Xia Jin Ejuan Zhang Huimin Yan 2022-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010149 https://doaj.org/article/84783e9f302f4b038b02f8354901c6c2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010149 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010149 https://doaj.org/article/84783e9f302f4b038b02f8354901c6c2 PLoS Neglected Tropical Diseases, Vol 16, Iss 1, p e0010149 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010149 2022-12-31T16:01:42Z Chikungunya virus (CHIKV) is an emerging mosquito-transmitted alphavirus that leads to acute fever and chronic debilitating polyarthralgia. To date, the mechanism underlying chronic recurrent arthralgia is unknown. In the present study, newborn wild-type C57BL/6 mice were infected with CHIKV, and the virological and pathological features of CHIKV infection were analyzed over a period of 50 days. Acute viral infection was readily established by footpad inoculation of CHIKV at doses ranging from 10 plaque forming unit (PFU) to 106 PFU, during which inoculation dose-dependent viral RNA and skeletal muscle damage were detected in the foot tissues. However, persistent CHIKV was observed only when the mice were infected with a high dose of 106 PFU of CHIKV, in which low copy numbers (103-104) of viral positive strand RNA were continuously detectable in the feet from 29 to 50 dpi, along with a low level and progressive reduction in virus-specific CD8+ T cell responses. In contrast, viral negative strand RNA was detected at 50 dpi but not at 29 dpi and was accompanied by significant local skeletal muscle damage at 50 dpi when mild synovial hyperplasia appeared in the foot joints, although the damage was briefly repaired at 29 dpi. These results demonstrated that a high viral inoculation dose leads to viral persistence and progression to chronic tissue damage after recovery from acute infection. Taken together, these results provide a useful tool for elucidating the pathogenesis of persistent CHIKV infection and viral relapse-associated chronic arthritis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 1 e0010149 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yue Zhang Hu Yan Xian Li Dihan Zhou Maohua Zhong Jingyi Yang Bali Zhao Xuxu Fan Jun Fan Jiayi Shu Mengji Lu Xia Jin Ejuan Zhang Huimin Yan A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chikungunya virus (CHIKV) is an emerging mosquito-transmitted alphavirus that leads to acute fever and chronic debilitating polyarthralgia. To date, the mechanism underlying chronic recurrent arthralgia is unknown. In the present study, newborn wild-type C57BL/6 mice were infected with CHIKV, and the virological and pathological features of CHIKV infection were analyzed over a period of 50 days. Acute viral infection was readily established by footpad inoculation of CHIKV at doses ranging from 10 plaque forming unit (PFU) to 106 PFU, during which inoculation dose-dependent viral RNA and skeletal muscle damage were detected in the foot tissues. However, persistent CHIKV was observed only when the mice were infected with a high dose of 106 PFU of CHIKV, in which low copy numbers (103-104) of viral positive strand RNA were continuously detectable in the feet from 29 to 50 dpi, along with a low level and progressive reduction in virus-specific CD8+ T cell responses. In contrast, viral negative strand RNA was detected at 50 dpi but not at 29 dpi and was accompanied by significant local skeletal muscle damage at 50 dpi when mild synovial hyperplasia appeared in the foot joints, although the damage was briefly repaired at 29 dpi. These results demonstrated that a high viral inoculation dose leads to viral persistence and progression to chronic tissue damage after recovery from acute infection. Taken together, these results provide a useful tool for elucidating the pathogenesis of persistent CHIKV infection and viral relapse-associated chronic arthritis. |
format |
Article in Journal/Newspaper |
author |
Yue Zhang Hu Yan Xian Li Dihan Zhou Maohua Zhong Jingyi Yang Bali Zhao Xuxu Fan Jun Fan Jiayi Shu Mengji Lu Xia Jin Ejuan Zhang Huimin Yan |
author_facet |
Yue Zhang Hu Yan Xian Li Dihan Zhou Maohua Zhong Jingyi Yang Bali Zhao Xuxu Fan Jun Fan Jiayi Shu Mengji Lu Xia Jin Ejuan Zhang Huimin Yan |
author_sort |
Yue Zhang |
title |
A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
title_short |
A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
title_full |
A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
title_fullStr |
A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
title_full_unstemmed |
A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
title_sort |
high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010149 https://doaj.org/article/84783e9f302f4b038b02f8354901c6c2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 1, p e0010149 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010149 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010149 https://doaj.org/article/84783e9f302f4b038b02f8354901c6c2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010149 |
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PLOS Neglected Tropical Diseases |
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16 |
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1 |
container_start_page |
e0010149 |
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1766342325491990528 |