A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis.
Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy and epidemiological studies. Common approaches for Leishmania detection involve the invasive collection of specimens for direct identification of amastigotes by microscopy and the culturing of promastigotes from infecte...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005750 https://doaj.org/article/84656254bf9742dc96789fe9950eafe4 |
id |
ftdoajarticles:oai:doaj.org/article:84656254bf9742dc96789fe9950eafe4 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:84656254bf9742dc96789fe9950eafe4 2023-05-15T15:09:39+02:00 A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. Yasaman Taslimi Pardis Sadeghipour Sima Habibzadeh Vahid Mashayekhi Hossien Mortazavi Ingrid Müller Majella E Lane Pascale Kropf Sima Rafati 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005750 https://doaj.org/article/84656254bf9742dc96789fe9950eafe4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5526608?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005750 https://doaj.org/article/84656254bf9742dc96789fe9950eafe4 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005750 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005750 2022-12-31T13:04:59Z Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy and epidemiological studies. Common approaches for Leishmania detection involve the invasive collection of specimens for direct identification of amastigotes by microscopy and the culturing of promastigotes from infected tissues. Although these techniques are highly specific, they require highly skilled health workers and have the inherent risks of all invasive procedures, such as pain and risk of bacterial and fungal super-infection. Therefore, it is essential to reduce discomfort, potential infection and scarring caused by invasive diagnostic approaches especially for children. In this report, we present a novel non-invasive method, that is painless, rapid and user-friendly, using sequential tape strips for sampling and isolation of DNA from the surface of active and healed skin lesions of CL patients. A total of 119 patients suspected of suffering from cutaneous leishmaniasis with different clinical manifestations were recruited and samples were collected both from their lesions and from uninfected areas. In addition, 15 fungal-infected lesions and 54 areas of healthy skin were examined. The duration of sampling is short (less than one minute) and species identification by PCR is highly specific and sensitive. The sequential tape stripping sampling method is a sensitive, non-invasive and cost-effective alternative to traditional diagnostic assays and it is suitable for field studies as well as for use in health care centers. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005750 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yasaman Taslimi Pardis Sadeghipour Sima Habibzadeh Vahid Mashayekhi Hossien Mortazavi Ingrid Müller Majella E Lane Pascale Kropf Sima Rafati A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy and epidemiological studies. Common approaches for Leishmania detection involve the invasive collection of specimens for direct identification of amastigotes by microscopy and the culturing of promastigotes from infected tissues. Although these techniques are highly specific, they require highly skilled health workers and have the inherent risks of all invasive procedures, such as pain and risk of bacterial and fungal super-infection. Therefore, it is essential to reduce discomfort, potential infection and scarring caused by invasive diagnostic approaches especially for children. In this report, we present a novel non-invasive method, that is painless, rapid and user-friendly, using sequential tape strips for sampling and isolation of DNA from the surface of active and healed skin lesions of CL patients. A total of 119 patients suspected of suffering from cutaneous leishmaniasis with different clinical manifestations were recruited and samples were collected both from their lesions and from uninfected areas. In addition, 15 fungal-infected lesions and 54 areas of healthy skin were examined. The duration of sampling is short (less than one minute) and species identification by PCR is highly specific and sensitive. The sequential tape stripping sampling method is a sensitive, non-invasive and cost-effective alternative to traditional diagnostic assays and it is suitable for field studies as well as for use in health care centers. |
format |
Article in Journal/Newspaper |
author |
Yasaman Taslimi Pardis Sadeghipour Sima Habibzadeh Vahid Mashayekhi Hossien Mortazavi Ingrid Müller Majella E Lane Pascale Kropf Sima Rafati |
author_facet |
Yasaman Taslimi Pardis Sadeghipour Sima Habibzadeh Vahid Mashayekhi Hossien Mortazavi Ingrid Müller Majella E Lane Pascale Kropf Sima Rafati |
author_sort |
Yasaman Taslimi |
title |
A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
title_short |
A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
title_full |
A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
title_fullStr |
A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
title_full_unstemmed |
A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
title_sort |
novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005750 https://doaj.org/article/84656254bf9742dc96789fe9950eafe4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005750 (2017) |
op_relation |
http://europepmc.org/articles/PMC5526608?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005750 https://doaj.org/article/84656254bf9742dc96789fe9950eafe4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005750 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
7 |
container_start_page |
e0005750 |
_version_ |
1766340805565349888 |