Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response
IκB kinases (IKKs) play critical roles in innate immunity through signal-induced activation of the key transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factors (IRFs). However, studies of invertebrate IKK functions remain scarce. In this study, we performed phylogenetic anal...
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ftdoajarticles:oai:doaj.org/article:844ad2f9d4ab43a0a7830cc9c239f3fa 2023-05-15T15:59:07+02:00 Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang 2019-07-01T00:00:00Z https://doi.org/10.3389/fimmu.2019.01826 https://doaj.org/article/844ad2f9d4ab43a0a7830cc9c239f3fa EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2019.01826/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2019.01826 https://doaj.org/article/844ad2f9d4ab43a0a7830cc9c239f3fa Frontiers in Immunology, Vol 10 (2019) Crassostrea gigas innate immunity IKKα IKKβ Toll-like receptor RIG-I-like receptor Immunologic diseases. Allergy RC581-607 article 2019 ftdoajarticles https://doi.org/10.3389/fimmu.2019.01826 2022-12-30T20:58:47Z IκB kinases (IKKs) play critical roles in innate immunity through signal-induced activation of the key transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factors (IRFs). However, studies of invertebrate IKK functions remain scarce. In this study, we performed phylogenetic analysis of IKKs and IKK-related kinases encoded in the Pacific oyster genome. We then cloned and characterized the oyster IKKα/β-2 gene. We found that oyster IKKα/β-2, a homolog of human IKKα/IKKβ, responded to challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), and polyinosinic-polycytidylic acid [poly(I:C)]. As a versatile immune molecule, IKKα/β-2 activated the promoters of NF-κB, TNFα, and IFNβ, as well as IFN-stimulated response element (ISRE)-containing promoters, initiating an antibacterial or antiviral immune state in mammalian cells. Importantly, together with the cloned oyster IKKα/β-1, we investigated the signal transduction pathways mediated by these two IKKα/β proteins. Our results showed that IKKα/β-1 and IKKα/β-2 could interact with the oyster TNF receptor-associated factor 6 (TRAF6) and that IKKα/β-2 could also bind to the oyster myeloid differentiation factor 88 (MyD88) protein directly, suggesting that oyster IKKα/βs participate in both RIG-I-like receptor (RLR) and Toll-like receptor (TLR) signaling for the reception of upstream immune signals. The fact that IKKα/β-1 and IKKα/β-2 formed homodimers by interacting with themselves and heterodimers by interacting with each other, along with the fact that both oyster IKKα/β proteins interacted with NEMO protein, indicates that oyster IKKα/βs and the scaffold protein NEMO form an IKK complex, which may be a key step in phosphorylating IκB proteins and activating NF-κB. Moreover, we found that oyster IKKα/βs could interact with IRF8, and this may be related to the IKK-mediated activation of ISRE promotors and their involvement in the oyster “interferon (IFN)-like” antiviral pathway. Moreover, the expression of oyster IKKα/β-1 and IKKα/β-2 may induce ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Immunology 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Crassostrea gigas innate immunity IKKα IKKβ Toll-like receptor RIG-I-like receptor Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Crassostrea gigas innate immunity IKKα IKKβ Toll-like receptor RIG-I-like receptor Immunologic diseases. Allergy RC581-607 Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response |
topic_facet |
Crassostrea gigas innate immunity IKKα IKKβ Toll-like receptor RIG-I-like receptor Immunologic diseases. Allergy RC581-607 |
description |
IκB kinases (IKKs) play critical roles in innate immunity through signal-induced activation of the key transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factors (IRFs). However, studies of invertebrate IKK functions remain scarce. In this study, we performed phylogenetic analysis of IKKs and IKK-related kinases encoded in the Pacific oyster genome. We then cloned and characterized the oyster IKKα/β-2 gene. We found that oyster IKKα/β-2, a homolog of human IKKα/IKKβ, responded to challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), and polyinosinic-polycytidylic acid [poly(I:C)]. As a versatile immune molecule, IKKα/β-2 activated the promoters of NF-κB, TNFα, and IFNβ, as well as IFN-stimulated response element (ISRE)-containing promoters, initiating an antibacterial or antiviral immune state in mammalian cells. Importantly, together with the cloned oyster IKKα/β-1, we investigated the signal transduction pathways mediated by these two IKKα/β proteins. Our results showed that IKKα/β-1 and IKKα/β-2 could interact with the oyster TNF receptor-associated factor 6 (TRAF6) and that IKKα/β-2 could also bind to the oyster myeloid differentiation factor 88 (MyD88) protein directly, suggesting that oyster IKKα/βs participate in both RIG-I-like receptor (RLR) and Toll-like receptor (TLR) signaling for the reception of upstream immune signals. The fact that IKKα/β-1 and IKKα/β-2 formed homodimers by interacting with themselves and heterodimers by interacting with each other, along with the fact that both oyster IKKα/β proteins interacted with NEMO protein, indicates that oyster IKKα/βs and the scaffold protein NEMO form an IKK complex, which may be a key step in phosphorylating IκB proteins and activating NF-κB. Moreover, we found that oyster IKKα/βs could interact with IRF8, and this may be related to the IKK-mediated activation of ISRE promotors and their involvement in the oyster “interferon (IFN)-like” antiviral pathway. Moreover, the expression of oyster IKKα/β-1 and IKKα/β-2 may induce ... |
format |
Article in Journal/Newspaper |
author |
Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang |
author_facet |
Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang |
author_sort |
Baoyu Huang |
title |
Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response |
title_short |
Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response |
title_full |
Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response |
title_fullStr |
Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response |
title_full_unstemmed |
Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response |
title_sort |
oyster versatile ikkα/βs are involved in toll-like receptor and rig-i-like receptor signaling for innate immune response |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fimmu.2019.01826 https://doaj.org/article/844ad2f9d4ab43a0a7830cc9c239f3fa |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01826/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2019.01826 https://doaj.org/article/844ad2f9d4ab43a0a7830cc9c239f3fa |
op_doi |
https://doi.org/10.3389/fimmu.2019.01826 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
_version_ |
1766394909964632064 |