Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice
Trypanosoma cruzi is usually transmitted by contact with the excreta of infected Triatominae; among non-vectorial infections, direct transmission through coitus has been proposed. We investigated this possibility by instilling, through the external meatus of the vagina and the penis of previously an...
Published in: | Memórias do Instituto Oswaldo Cruz |
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ftdoajarticles:oai:doaj.org/article:840def5c0ea8477682d4f62875e0ea20 2023-10-01T03:59:06+02:00 Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice Leidi Herrera Servio Urdaneta-Morales 2001-07-01T00:00:00Z https://doi.org/10.1590/S0074-02762001000500024 https://doaj.org/article/840def5c0ea8477682d4f62875e0ea20 EN eng Fundação Oswaldo Cruz (FIOCRUZ) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000500024 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762001000500024 0074-0276 1678-8060 https://doaj.org/article/840def5c0ea8477682d4f62875e0ea20 Memorias do Instituto Oswaldo Cruz, Vol 96, Iss 5, Pp 713-717 (2001) Trypanosoma cruzi Didelphis marsupialis Rattus rattus genital transmission histotropism cyclophosphamide Microbiology QR1-502 Infectious and parasitic diseases RC109-216 article 2001 ftdoajarticles https://doi.org/10.1590/S0074-02762001000500024 2023-09-03T00:42:30Z Trypanosoma cruzi is usually transmitted by contact with the excreta of infected Triatominae; among non-vectorial infections, direct transmission through coitus has been proposed. We investigated this possibility by instilling, through the external meatus of the vagina and the penis of previously anesthetized NMRI albino mice, blood of mice infected with strains isolated from Didelphis marsupialis (opossum, strain CO57), Rattus rattus (rat, strain CO22) and human (strain EP). Some animals were allowed to copulate the same day of the instillation. In other experiments, the strains were inoculated in the scrotum. To determine the effect of immunosuppression, some mice were treated with cyclophosphamide 30 days post-instillation. Controls were instilled orally and ocularly. Vaginal instillation with strain CO22 produced systemic infection with tropism to the heart, skeletal muscle, skin, duodenum, pancreas, ovary and sternum. Scrotal inoculation with strain EP likewise invaded liver, spleen, lung, lymph nodes and urogenital organs; while strain CO57 invaded skeletal and cardiac muscle, pancreas, testis, and vas deferens. Penile infection with strain CO22 was detected by xenodiagnosis. Immunosuppression did not increase parasitemia of vaginally infected mice or controls. Mating did not produce infection. Our results show that contact of blood trypomastigotes of T. cruzi with genital mucosa can produce blood and tissue infections. These results are discussed in relation to reports of frequent experimental tropism of T. cruzi toward urogenital organs. Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles Memórias do Instituto Oswaldo Cruz 96 5 713 717 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Trypanosoma cruzi Didelphis marsupialis Rattus rattus genital transmission histotropism cyclophosphamide Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Trypanosoma cruzi Didelphis marsupialis Rattus rattus genital transmission histotropism cyclophosphamide Microbiology QR1-502 Infectious and parasitic diseases RC109-216 Leidi Herrera Servio Urdaneta-Morales Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice |
topic_facet |
Trypanosoma cruzi Didelphis marsupialis Rattus rattus genital transmission histotropism cyclophosphamide Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
description |
Trypanosoma cruzi is usually transmitted by contact with the excreta of infected Triatominae; among non-vectorial infections, direct transmission through coitus has been proposed. We investigated this possibility by instilling, through the external meatus of the vagina and the penis of previously anesthetized NMRI albino mice, blood of mice infected with strains isolated from Didelphis marsupialis (opossum, strain CO57), Rattus rattus (rat, strain CO22) and human (strain EP). Some animals were allowed to copulate the same day of the instillation. In other experiments, the strains were inoculated in the scrotum. To determine the effect of immunosuppression, some mice were treated with cyclophosphamide 30 days post-instillation. Controls were instilled orally and ocularly. Vaginal instillation with strain CO22 produced systemic infection with tropism to the heart, skeletal muscle, skin, duodenum, pancreas, ovary and sternum. Scrotal inoculation with strain EP likewise invaded liver, spleen, lung, lymph nodes and urogenital organs; while strain CO57 invaded skeletal and cardiac muscle, pancreas, testis, and vas deferens. Penile infection with strain CO22 was detected by xenodiagnosis. Immunosuppression did not increase parasitemia of vaginally infected mice or controls. Mating did not produce infection. Our results show that contact of blood trypomastigotes of T. cruzi with genital mucosa can produce blood and tissue infections. These results are discussed in relation to reports of frequent experimental tropism of T. cruzi toward urogenital organs. |
format |
Article in Journal/Newspaper |
author |
Leidi Herrera Servio Urdaneta-Morales |
author_facet |
Leidi Herrera Servio Urdaneta-Morales |
author_sort |
Leidi Herrera |
title |
Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice |
title_short |
Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice |
title_full |
Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice |
title_fullStr |
Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice |
title_full_unstemmed |
Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice |
title_sort |
experimental transmission of trypanosoma cruzi through the genitalia of albino mice |
publisher |
Fundação Oswaldo Cruz (FIOCRUZ) |
publishDate |
2001 |
url |
https://doi.org/10.1590/S0074-02762001000500024 https://doaj.org/article/840def5c0ea8477682d4f62875e0ea20 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Memorias do Instituto Oswaldo Cruz, Vol 96, Iss 5, Pp 713-717 (2001) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000500024 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762001000500024 0074-0276 1678-8060 https://doaj.org/article/840def5c0ea8477682d4f62875e0ea20 |
op_doi |
https://doi.org/10.1590/S0074-02762001000500024 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
96 |
container_issue |
5 |
container_start_page |
713 |
op_container_end_page |
717 |
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