Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection.
Paracoccidioidomycosis (PCM) is the most prevalent deep mycosis in Latin America and is caused by fungi from the Paracoccidioides genus. Virulence factors are important fungal characteristics that support the development of disease. Aspartyl proteases (Saps) are virulence factors in many human funga...
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ftdoajarticles:oai:doaj.org/article:839de806725a407f83ed4f7a29e92229 2023-05-15T15:04:00+02:00 Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. Daniele Gonçalves Castilho Alison Felipe Alencar Chaves Marina Valente Navarro Palloma Mendes Conceição Karen Spadari Ferreira Luiz Severino da Silva Patricia Xander Wagner Luiz Batista 2018-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006806 https://doaj.org/article/839de806725a407f83ed4f7a29e92229 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6177206?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006806 https://doaj.org/article/839de806725a407f83ed4f7a29e92229 PLoS Neglected Tropical Diseases, Vol 12, Iss 9, p e0006806 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006806 2022-12-31T00:59:18Z Paracoccidioidomycosis (PCM) is the most prevalent deep mycosis in Latin America and is caused by fungi from the Paracoccidioides genus. Virulence factors are important fungal characteristics that support the development of disease. Aspartyl proteases (Saps) are virulence factors in many human fungal pathogens that play an important role in the host invasion process. We report here that immunization with recombinant Sap from Paracoccidioides brasiliensis (rPbSap) imparted a protective effect in an experimental PCM model. The rPbSap-immunized mice had decreased fungal loads, and their lung parenchyma were notably preserved. An aspartyl protease inhibitor (pepstatin A) significantly decreased pulmonary injury and reduced fungal loads in the lung. Additionally, we observed that pepstatin A enhanced the fungicidal and phagocytic profile of macrophages against P. brasiliensis. Furthermore, PbSAP expression was highly altered by environmental conditions, including thermal stress, dimorphism switching and low pH. Hence, our data suggest that PbSap is an important virulence regulator in P. brasiliensis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 9 e0006806 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniele Gonçalves Castilho Alison Felipe Alencar Chaves Marina Valente Navarro Palloma Mendes Conceição Karen Spadari Ferreira Luiz Severino da Silva Patricia Xander Wagner Luiz Batista Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Paracoccidioidomycosis (PCM) is the most prevalent deep mycosis in Latin America and is caused by fungi from the Paracoccidioides genus. Virulence factors are important fungal characteristics that support the development of disease. Aspartyl proteases (Saps) are virulence factors in many human fungal pathogens that play an important role in the host invasion process. We report here that immunization with recombinant Sap from Paracoccidioides brasiliensis (rPbSap) imparted a protective effect in an experimental PCM model. The rPbSap-immunized mice had decreased fungal loads, and their lung parenchyma were notably preserved. An aspartyl protease inhibitor (pepstatin A) significantly decreased pulmonary injury and reduced fungal loads in the lung. Additionally, we observed that pepstatin A enhanced the fungicidal and phagocytic profile of macrophages against P. brasiliensis. Furthermore, PbSAP expression was highly altered by environmental conditions, including thermal stress, dimorphism switching and low pH. Hence, our data suggest that PbSap is an important virulence regulator in P. brasiliensis. |
format |
Article in Journal/Newspaper |
author |
Daniele Gonçalves Castilho Alison Felipe Alencar Chaves Marina Valente Navarro Palloma Mendes Conceição Karen Spadari Ferreira Luiz Severino da Silva Patricia Xander Wagner Luiz Batista |
author_facet |
Daniele Gonçalves Castilho Alison Felipe Alencar Chaves Marina Valente Navarro Palloma Mendes Conceição Karen Spadari Ferreira Luiz Severino da Silva Patricia Xander Wagner Luiz Batista |
author_sort |
Daniele Gonçalves Castilho |
title |
Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. |
title_short |
Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. |
title_full |
Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. |
title_fullStr |
Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. |
title_full_unstemmed |
Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection. |
title_sort |
secreted aspartyl proteinase (pbsap) contributes to the virulence of paracoccidioides brasiliensis infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006806 https://doaj.org/article/839de806725a407f83ed4f7a29e92229 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 9, p e0006806 (2018) |
op_relation |
http://europepmc.org/articles/PMC6177206?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006806 https://doaj.org/article/839de806725a407f83ed4f7a29e92229 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006806 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
9 |
container_start_page |
e0006806 |
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1766335835539505152 |