CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro
Conditions for induction of callus formation from root and stem explants and long-term maintenance of Deschampsia antarctica Desv. Tissue culture have been specified. Ability to callus formation depended on mineral composition of nutrient medium, combination of growth regulator concentrations, place...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013
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ftdoajarticles:oai:doaj.org/article:8390b288aad14c83bbe0a5a5bd5a26b3 2023-10-09T21:46:39+02:00 CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro О. M. Zagrychuk A. I. Herts N. M. Drobyk V. A. Kunakh 2013-12-01T00:00:00Z https://doi.org/10.15407/biotech6.06.077 https://doaj.org/article/8390b288aad14c83bbe0a5a5bd5a26b3 EN UK eng ukr National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry http://biotechnology.kiev.ua/images/storage/no6_2013/zagrychuk_6_2013.pdf https://doaj.org/toc/2410-7751 https://doaj.org/toc/2410-776X doi:10.15407/biotech6.06.077 2410-7751 2410-776X https://doaj.org/article/8390b288aad14c83bbe0a5a5bd5a26b3 Biotechnologia Acta, Vol 6, Iss 6, Pp 77-95 (2013) Deschampsia antarctica Desv callus formation callus culture spontaneous indirect regeneration in vitro regenerated plants Biotechnology TP248.13-248.65 article 2013 ftdoajarticles https://doi.org/10.15407/biotech6.06.077 2023-09-10T00:50:03Z Conditions for induction of callus formation from root and stem explants and long-term maintenance of Deschampsia antarctica Desv. Tissue culture have been specified. Ability to callus formation depended on mineral composition of nutrient medium, combination of growth regulator concentrations, place of donor-plant vegetation and type of explant. The optimal for callus tissue generation was Gamborg, Eveleigh — B5 nutrient medium, supplemented with 0.9–1.0 mg/l 2.4-dichlorophenyl acetic acid and 0.09–0.1 mg/l of cytokinine benzylaminopurine. Callus formation potency from the root explants considerably exceeded (1.5–2 times) that of from stem ones. The shoots were derived through spontaneous indirect organogenesis. Regeneration efficiency was found to be affected by nutrient medium composition and callus origin. Regenerated shoots were rooted and conditions for growth of regenerated plants in vitro were specified. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Biotechnologia Acta 6 6 77 85 |
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language |
English Ukrainian |
topic |
Deschampsia antarctica Desv callus formation callus culture spontaneous indirect regeneration in vitro regenerated plants Biotechnology TP248.13-248.65 |
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Deschampsia antarctica Desv callus formation callus culture spontaneous indirect regeneration in vitro regenerated plants Biotechnology TP248.13-248.65 О. M. Zagrychuk A. I. Herts N. M. Drobyk V. A. Kunakh CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro |
topic_facet |
Deschampsia antarctica Desv callus formation callus culture spontaneous indirect regeneration in vitro regenerated plants Biotechnology TP248.13-248.65 |
description |
Conditions for induction of callus formation from root and stem explants and long-term maintenance of Deschampsia antarctica Desv. Tissue culture have been specified. Ability to callus formation depended on mineral composition of nutrient medium, combination of growth regulator concentrations, place of donor-plant vegetation and type of explant. The optimal for callus tissue generation was Gamborg, Eveleigh — B5 nutrient medium, supplemented with 0.9–1.0 mg/l 2.4-dichlorophenyl acetic acid and 0.09–0.1 mg/l of cytokinine benzylaminopurine. Callus formation potency from the root explants considerably exceeded (1.5–2 times) that of from stem ones. The shoots were derived through spontaneous indirect organogenesis. Regeneration efficiency was found to be affected by nutrient medium composition and callus origin. Regenerated shoots were rooted and conditions for growth of regenerated plants in vitro were specified. |
format |
Article in Journal/Newspaper |
author |
О. M. Zagrychuk A. I. Herts N. M. Drobyk V. A. Kunakh |
author_facet |
О. M. Zagrychuk A. I. Herts N. M. Drobyk V. A. Kunakh |
author_sort |
О. M. Zagrychuk |
title |
CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro |
title_short |
CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro |
title_full |
CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro |
title_fullStr |
CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro |
title_full_unstemmed |
CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro |
title_sort |
callus formation and regeneration of deschampsia antarctica desv. (poaceae) in culture in vitro |
publisher |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
publishDate |
2013 |
url |
https://doi.org/10.15407/biotech6.06.077 https://doaj.org/article/8390b288aad14c83bbe0a5a5bd5a26b3 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnologia Acta, Vol 6, Iss 6, Pp 77-95 (2013) |
op_relation |
http://biotechnology.kiev.ua/images/storage/no6_2013/zagrychuk_6_2013.pdf https://doaj.org/toc/2410-7751 https://doaj.org/toc/2410-776X doi:10.15407/biotech6.06.077 2410-7751 2410-776X https://doaj.org/article/8390b288aad14c83bbe0a5a5bd5a26b3 |
op_doi |
https://doi.org/10.15407/biotech6.06.077 |
container_title |
Biotechnologia Acta |
container_volume |
6 |
container_issue |
6 |
container_start_page |
77 |
op_container_end_page |
85 |
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1779309123541663744 |