CALLUS FORMATION AND REGENERATION OF Deschampsia antarctica Desv. (Poaceae) IN CULTURE in vitro

• Published in: Biotechnologia Acta
• Main Authors: О. M. Zagrychuk, A. I. Herts, N. M. Drobyk, V. A. Kunakh
• Format: Article in Journal/Newspaper
• Language: English; Ukrainian
• Published: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 2013
• Subjects: Deschampsia antarctica Desv; callus formation; callus culture; spontaneous indirect regeneration in vitro; regenerated plants; Biotechnology; TP248.13-248.65; Antarc*; Antarctica
• Online Access: https://doi.org/10.15407/biotech6.06.077; https://doaj.org/article/8390b288aad14c83bbe0a5a5bd5a26b3

Conditions for induction of callus formation from root and stem explants and long-term maintenance of Deschampsia antarctica Desv. Tissue culture have been specified. Ability to callus formation depended on mineral composition of nutrient medium, combination of growth regulator concentrations, place of donor-plant vegetation and type of explant. The optimal for callus tissue generation was Gamborg, Eveleigh — B5 nutrient medium, supplemented with 0.9–1.0 mg/l 2.4-dichlorophenyl acetic acid and 0.09–0.1 mg/l of cytokinine benzylaminopurine. Callus formation potency from the root explants considerably exceeded (1.5–2 times) that of from stem ones. The shoots were derived through spontaneous indirect organogenesis. Regeneration efficiency was found to be affected by nutrient medium composition and callus origin. Regenerated shoots were rooted and conditions for growth of regenerated plants in vitro were specified.