Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations
Abstract Background: Advancements in proteomics, including the technological improvement in instrumentation, have turned mass spectrometry into an indispensable tool in the study of venoms and toxins. In addition, the advance of nanoscale liquid chromatography coupled to nanoelectrospray mass spectr...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2018
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s40409-018-0141-3 https://doaj.org/article/838d6d4b994e4c0e92807a18574e752d |
id |
ftdoajarticles:oai:doaj.org/article:838d6d4b994e4c0e92807a18574e752d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:838d6d4b994e4c0e92807a18574e752d 2023-05-15T15:16:46+02:00 Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations Camila Takeno Cologna Renata Santos Rodrigues Jean Santos Edwin de Pauw Eliane Candiani Arantes Loïc Quinton 2018-03-01T00:00:00Z https://doi.org/10.1186/s40409-018-0141-3 https://doaj.org/article/838d6d4b994e4c0e92807a18574e752d EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100303&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-018-0141-3 https://doaj.org/article/838d6d4b994e4c0e92807a18574e752d Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 0 (2018) Ant venom Peptidomics Ponericins Venom comparison Antimicrobial peptides Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2018 ftdoajarticles https://doi.org/10.1186/s40409-018-0141-3 2022-12-31T15:53:13Z Abstract Background: Advancements in proteomics, including the technological improvement in instrumentation, have turned mass spectrometry into an indispensable tool in the study of venoms and toxins. In addition, the advance of nanoscale liquid chromatography coupled to nanoelectrospray mass spectrometry allows, due to its high sensitivity, the study of venoms from species previously left aside, such as ants. Ant venoms are a complex mixture of compounds used for defense, predation or communication purposes. The venom from Neoponera ants, a genus restricted to Neotropical regions, is known to have cytolytic, hemolytic, antimicrobial and insecticidal activities. Moreover, venoms from several Neoponera species have been compared and differences in their toxicity related to nesting habitat variation were reported. Therefore, the present study aimed to perform a deep peptidomic analysis of Neoponera villosa venom and a comparison of seasonal and nesting habitat variations using high-resolution mass spectrometry. Methods: Specimens of N. villosa ants were captured in Panga Natural Reserve (Uberlândia, MG, Brazil) from arboreal and ground-dwelling nests during summer and winter time. The venom glands were dissected, pooled and disrupted by ultra-sonic waves. The venom collected from different habitats (arboreal and ground-dwelling) and different seasons (summer and winter) was injected into a nanoACQUITY ULPC hyphened to a Q-Exactive Orbitrap mass spectrometer. The raw data were analyzed using PEAKS 7. Results: The results showed a molecular diversity of more than 500 peptides among these venoms, mostly in the mass range of 800–4000 Da. Mutations and post-translational modifications were described and differences among the venoms were observed. Part of the peptides matched with ponericins, a well-known antimicrobial peptide family. In addition, smaller fragments related to ponericins were also identified, suggesting that this class of antimicrobial peptide might undergo enzymatic cleavages. Conclusion: There are ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 24 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ant venom Peptidomics Ponericins Venom comparison Antimicrobial peptides Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Ant venom Peptidomics Ponericins Venom comparison Antimicrobial peptides Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Camila Takeno Cologna Renata Santos Rodrigues Jean Santos Edwin de Pauw Eliane Candiani Arantes Loïc Quinton Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
topic_facet |
Ant venom Peptidomics Ponericins Venom comparison Antimicrobial peptides Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Advancements in proteomics, including the technological improvement in instrumentation, have turned mass spectrometry into an indispensable tool in the study of venoms and toxins. In addition, the advance of nanoscale liquid chromatography coupled to nanoelectrospray mass spectrometry allows, due to its high sensitivity, the study of venoms from species previously left aside, such as ants. Ant venoms are a complex mixture of compounds used for defense, predation or communication purposes. The venom from Neoponera ants, a genus restricted to Neotropical regions, is known to have cytolytic, hemolytic, antimicrobial and insecticidal activities. Moreover, venoms from several Neoponera species have been compared and differences in their toxicity related to nesting habitat variation were reported. Therefore, the present study aimed to perform a deep peptidomic analysis of Neoponera villosa venom and a comparison of seasonal and nesting habitat variations using high-resolution mass spectrometry. Methods: Specimens of N. villosa ants were captured in Panga Natural Reserve (Uberlândia, MG, Brazil) from arboreal and ground-dwelling nests during summer and winter time. The venom glands were dissected, pooled and disrupted by ultra-sonic waves. The venom collected from different habitats (arboreal and ground-dwelling) and different seasons (summer and winter) was injected into a nanoACQUITY ULPC hyphened to a Q-Exactive Orbitrap mass spectrometer. The raw data were analyzed using PEAKS 7. Results: The results showed a molecular diversity of more than 500 peptides among these venoms, mostly in the mass range of 800–4000 Da. Mutations and post-translational modifications were described and differences among the venoms were observed. Part of the peptides matched with ponericins, a well-known antimicrobial peptide family. In addition, smaller fragments related to ponericins were also identified, suggesting that this class of antimicrobial peptide might undergo enzymatic cleavages. Conclusion: There are ... |
format |
Article in Journal/Newspaper |
author |
Camila Takeno Cologna Renata Santos Rodrigues Jean Santos Edwin de Pauw Eliane Candiani Arantes Loïc Quinton |
author_facet |
Camila Takeno Cologna Renata Santos Rodrigues Jean Santos Edwin de Pauw Eliane Candiani Arantes Loïc Quinton |
author_sort |
Camila Takeno Cologna |
title |
Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
title_short |
Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
title_full |
Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
title_fullStr |
Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
title_full_unstemmed |
Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
title_sort |
peptidomic investigation of neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations |
publisher |
SciELO |
publishDate |
2018 |
url |
https://doi.org/10.1186/s40409-018-0141-3 https://doaj.org/article/838d6d4b994e4c0e92807a18574e752d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 0 (2018) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100303&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-018-0141-3 https://doaj.org/article/838d6d4b994e4c0e92807a18574e752d |
op_doi |
https://doi.org/10.1186/s40409-018-0141-3 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
24 |
container_issue |
1 |
_version_ |
1766347069412343808 |