Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria
Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty...
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ftdoajarticles:oai:doaj.org/article:837e293aae5440d2b279a863aa607259 2023-05-15T14:43:55+02:00 Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria Yu-Zhong Zhang Xiu-Lan Chen Xiao-Yan Song Mei Shi Xi-Ying Zhang Jie Yang Sheng Dong 2012-11-01T00:00:00Z https://doi.org/10.3390/md10112481 https://doaj.org/article/837e293aae5440d2b279a863aa607259 EN eng MDPI AG http://www.mdpi.com/1660-3397/10/11/2481 https://doaj.org/toc/1660-3397 doi:10.3390/md10112481 1660-3397 https://doaj.org/article/837e293aae5440d2b279a863aa607259 Marine Drugs, Vol 10, Iss 11, Pp 2481-2491 (2012) alginate lyase-excreting bacteria psychrophilic Arctic Laminaria diversity Biology (General) QH301-705.5 article 2012 ftdoajarticles https://doi.org/10.3390/md10112481 2022-12-30T19:57:07Z Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychrobacter (33/65), Psychromonas (10/65) and Polaribacter (8/65) were the predominant groups. Moreover, 21 alginate lyase-excreting strains were further screened from these Laminaria-associated bacteria. These alginate lyase-excreting strains belong to five genera. Psychromonas (8/21), Psedoalteromonas (6/21) and Polaribacter (4/21) are the predominant genera, and Psychrobacter, Winogradskyella, Psychromonas and Polaribacter were first found to produce alginate lyases. The optimal temperatures for the growth and algiante lyase production of many strains were as low as 10–20 °C, indicating that they are psychrophilic bacteria. The alginate lyases produced by 11 strains showed the highest activity at 20–30 °C, indicating that these enzymes are cold-adapted enzymes. Some strians showed high levels of extracellular alginate lyase activity around 200 U/mL. These results suggest that these algiante lyase-excreting bacteria from the Arctic alga are good materials for studying bacterial cold-adapted alginate lyases. Article in Journal/Newspaper Arctic Arctic Ocean Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Marine Drugs 10 12 2481 2491 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
alginate lyase-excreting bacteria psychrophilic Arctic Laminaria diversity Biology (General) QH301-705.5 |
spellingShingle |
alginate lyase-excreting bacteria psychrophilic Arctic Laminaria diversity Biology (General) QH301-705.5 Yu-Zhong Zhang Xiu-Lan Chen Xiao-Yan Song Mei Shi Xi-Ying Zhang Jie Yang Sheng Dong Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
topic_facet |
alginate lyase-excreting bacteria psychrophilic Arctic Laminaria diversity Biology (General) QH301-705.5 |
description |
Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychrobacter (33/65), Psychromonas (10/65) and Polaribacter (8/65) were the predominant groups. Moreover, 21 alginate lyase-excreting strains were further screened from these Laminaria-associated bacteria. These alginate lyase-excreting strains belong to five genera. Psychromonas (8/21), Psedoalteromonas (6/21) and Polaribacter (4/21) are the predominant genera, and Psychrobacter, Winogradskyella, Psychromonas and Polaribacter were first found to produce alginate lyases. The optimal temperatures for the growth and algiante lyase production of many strains were as low as 10–20 °C, indicating that they are psychrophilic bacteria. The alginate lyases produced by 11 strains showed the highest activity at 20–30 °C, indicating that these enzymes are cold-adapted enzymes. Some strians showed high levels of extracellular alginate lyase activity around 200 U/mL. These results suggest that these algiante lyase-excreting bacteria from the Arctic alga are good materials for studying bacterial cold-adapted alginate lyases. |
format |
Article in Journal/Newspaper |
author |
Yu-Zhong Zhang Xiu-Lan Chen Xiao-Yan Song Mei Shi Xi-Ying Zhang Jie Yang Sheng Dong |
author_facet |
Yu-Zhong Zhang Xiu-Lan Chen Xiao-Yan Song Mei Shi Xi-Ying Zhang Jie Yang Sheng Dong |
author_sort |
Yu-Zhong Zhang |
title |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_short |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_full |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_fullStr |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_full_unstemmed |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_sort |
cultivable alginate lyase-excreting bacteria associated with the arctic brown alga laminaria |
publisher |
MDPI AG |
publishDate |
2012 |
url |
https://doi.org/10.3390/md10112481 https://doaj.org/article/837e293aae5440d2b279a863aa607259 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Marine Drugs, Vol 10, Iss 11, Pp 2481-2491 (2012) |
op_relation |
http://www.mdpi.com/1660-3397/10/11/2481 https://doaj.org/toc/1660-3397 doi:10.3390/md10112481 1660-3397 https://doaj.org/article/837e293aae5440d2b279a863aa607259 |
op_doi |
https://doi.org/10.3390/md10112481 |
container_title |
Marine Drugs |
container_volume |
10 |
container_issue |
12 |
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2481 |
op_container_end_page |
2491 |
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1766315496310833152 |