Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses.
Background Chikungunya virus (CHIKV) and O'nyong nyong virus (ONNV) are phylogenetically related alphaviruses in the Semliki Forest Virus (SFV) antigenic complex of the Togaviridae family. There are limited data on the circulation of these two viruses in Burkina Faso. The aim of our study was t...
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ftdoajarticles:oai:doaj.org/article:837db316d56049b9903b4a087d477925 2024-09-09T19:28:10+00:00 Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. Bachirou Tinto Brice Bicaba Thérèse Samdapawindé Kagoné John Kayiwa Ingrid Rabe Corinne Simone Collette Merle Alidou Zango Ahidjo Ayouba Sara Salinas Dramane Kania Yannick Simonin 2024-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011712 https://doaj.org/article/837db316d56049b9903b4a087d477925 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011712 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011712 https://doaj.org/article/837db316d56049b9903b4a087d477925 PLoS Neglected Tropical Diseases, Vol 18, Iss 6, p e0011712 (2024) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2024 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011712 2024-08-05T17:48:53Z Background Chikungunya virus (CHIKV) and O'nyong nyong virus (ONNV) are phylogenetically related alphaviruses in the Semliki Forest Virus (SFV) antigenic complex of the Togaviridae family. There are limited data on the circulation of these two viruses in Burkina Faso. The aim of our study was to assess their circulation in the country by determining seroprevalence to each of the viruses in blood donor samples and by retrospective molecular and serological testing of samples collected as part of national measles and rubella surveillance. Methodology/principal findings All blood donor samples were analyzed on the Luminex platform using CHIKV and ONNV E2 antigens. Patient samples collected during national measles-rubella surveillance were screened by an initial ELISA for CHIKV IgM (CHIKjj Detect IgM ELISA) at the national laboratory. The positive samples were then analyzed by a second ELISA test for CHIKV IgM (CDC MAC-ELISA) at the reference laboratory. Finally, samples that had IgM positive results for both ELISA tests and had sufficient residual volume were tested by plaque reduction neutralization testing (PRNT) for CHIKV and ONNV. These same patient samples were also analyzed by rRT-PCR for CHIKV. Among the blood donor specimens, 55.49% of the samples were positive for alphaviruses including both CHIKV and ONNV positive samples. Among patient samples collected as part of national measles and rubella surveillance, 3.09% were IgM positive for CHIKV, including 2.5% confirmed by PRNT. PRNT failed to demonstrate any ONNV infections in these samples. No samples tested by RT-qPCR. had detectable CHIKV RNA. Conclusions/significance Our results suggest that CHIKV and ONNV have been circulating in the population of Burkina Faso and may have been confused with malaria, dengue fever or other febrile diseases such as measles or rubella. Our study underscores the necessity to enhance arbovirus surveillance systems in Burkina Faso. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 18 6 e0011712 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bachirou Tinto Brice Bicaba Thérèse Samdapawindé Kagoné John Kayiwa Ingrid Rabe Corinne Simone Collette Merle Alidou Zango Ahidjo Ayouba Sara Salinas Dramane Kania Yannick Simonin Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Chikungunya virus (CHIKV) and O'nyong nyong virus (ONNV) are phylogenetically related alphaviruses in the Semliki Forest Virus (SFV) antigenic complex of the Togaviridae family. There are limited data on the circulation of these two viruses in Burkina Faso. The aim of our study was to assess their circulation in the country by determining seroprevalence to each of the viruses in blood donor samples and by retrospective molecular and serological testing of samples collected as part of national measles and rubella surveillance. Methodology/principal findings All blood donor samples were analyzed on the Luminex platform using CHIKV and ONNV E2 antigens. Patient samples collected during national measles-rubella surveillance were screened by an initial ELISA for CHIKV IgM (CHIKjj Detect IgM ELISA) at the national laboratory. The positive samples were then analyzed by a second ELISA test for CHIKV IgM (CDC MAC-ELISA) at the reference laboratory. Finally, samples that had IgM positive results for both ELISA tests and had sufficient residual volume were tested by plaque reduction neutralization testing (PRNT) for CHIKV and ONNV. These same patient samples were also analyzed by rRT-PCR for CHIKV. Among the blood donor specimens, 55.49% of the samples were positive for alphaviruses including both CHIKV and ONNV positive samples. Among patient samples collected as part of national measles and rubella surveillance, 3.09% were IgM positive for CHIKV, including 2.5% confirmed by PRNT. PRNT failed to demonstrate any ONNV infections in these samples. No samples tested by RT-qPCR. had detectable CHIKV RNA. Conclusions/significance Our results suggest that CHIKV and ONNV have been circulating in the population of Burkina Faso and may have been confused with malaria, dengue fever or other febrile diseases such as measles or rubella. Our study underscores the necessity to enhance arbovirus surveillance systems in Burkina Faso. |
format |
Article in Journal/Newspaper |
author |
Bachirou Tinto Brice Bicaba Thérèse Samdapawindé Kagoné John Kayiwa Ingrid Rabe Corinne Simone Collette Merle Alidou Zango Ahidjo Ayouba Sara Salinas Dramane Kania Yannick Simonin |
author_facet |
Bachirou Tinto Brice Bicaba Thérèse Samdapawindé Kagoné John Kayiwa Ingrid Rabe Corinne Simone Collette Merle Alidou Zango Ahidjo Ayouba Sara Salinas Dramane Kania Yannick Simonin |
author_sort |
Bachirou Tinto |
title |
Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. |
title_short |
Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. |
title_full |
Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. |
title_fullStr |
Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. |
title_full_unstemmed |
Co-circulation of two Alphaviruses in Burkina Faso: Chikungunya and O'nyong nyong viruses. |
title_sort |
co-circulation of two alphaviruses in burkina faso: chikungunya and o'nyong nyong viruses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2024 |
url |
https://doi.org/10.1371/journal.pntd.0011712 https://doaj.org/article/837db316d56049b9903b4a087d477925 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 18, Iss 6, p e0011712 (2024) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011712 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011712 https://doaj.org/article/837db316d56049b9903b4a087d477925 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011712 |
container_title |
PLOS Neglected Tropical Diseases |
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18 |
container_issue |
6 |
container_start_page |
e0011712 |
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1809897446853050368 |