Effects of acidification on the proteome during early development of Babylonia areolata

• Published in: FEBS Open Bio
• Main Authors: Guilan Di, Yanfei Li, Guorong Zhu, Xiaoyu Guo, Hui Li, Miaoqin Huang, Minghui Shen, Caihuan Ke
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2019
• Subjects: Babylonia areolata; larva; ocean acidification; pCO 2; proteomics; Biology (General); QH301-705.5; Ocean acidification
• Online Access: https://doi.org/10.1002/2211-5463.12695; https://doaj.org/article/8363b83b35b6438297bc56ba97accf87

Increases in atmospheric CO2 partial pressure have lowered seawater pH in marine ecosystems, a process called ocean acidification (OA). The effects of OA during the critical stages of larval development may have disastrous consequences for some marine species, including Babylonia areolata (Link 1807), a commercially important sea snail in China and South East Asia. To investigate how OA affects the proteome of Babylonia areolata, here we used label‐free proteomics to study protein changes in response to acidified (pH 7.6) or ambient seawater (pH 8.1) during three larvae developmental stages of B. areolata, namely, the veliger larvae before attachment (E1), veliger larvae after attachment (E2), and carnivorous juvenile snail (E3). In total, we identified 720 proteins. This result suggested that acidification seriously affects late veliger stage after attachment (E2). Further examination of the roles of differentially expressed proteins, which include glutaredoxin, heat‐shock protein 70, thioredoxin, catalase, cytochrome‐c‐oxidase, peroxiredoxin 6, troponin T, CaM kinase II alpha, proteasome subunit N3 and cathepsin L, will be important for understanding the molecular mechanisms underlying pH reduction.