Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
The objective of this new paper was to evaluate the enzymatic esterification reaction conducted in supercritical or near-critical CO2, catalyzed by immobilized lipase B from Candida antarctica (CALB). The biocatalyst was prepared through the immobilization of CALB by covalent attachment using chitos...
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ftdoajarticles:oai:doaj.org/article:8360bd5898bb47a89ba6e37703b27ad0 2023-05-15T13:45:53+02:00 Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide José C.S. dos Santos Horacio L. Bonazza Leonardo J.B.L. de Matos Elizabete A. Carneiro Oveimar Barbosa Roberto Fernandez-Lafuente Luciana R.B. Gonçalves Hosiberto B. de Sant’ Ana Rílvia S. Santiago-Aguiar 2017-03-01T00:00:00Z https://doi.org/10.1016/j.btre.2017.02.003 https://doaj.org/article/8360bd5898bb47a89ba6e37703b27ad0 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2215017X16300911 https://doaj.org/toc/2215-017X 2215-017X doi:10.1016/j.btre.2017.02.003 https://doaj.org/article/8360bd5898bb47a89ba6e37703b27ad0 Biotechnology Reports, Vol 14, Iss C, Pp 16-26 (2017) Lipase Immobilization Chitosan Fatty acid ethyl ester Esterification Supercritical fluids Biotechnology TP248.13-248.65 article 2017 ftdoajarticles https://doi.org/10.1016/j.btre.2017.02.003 2022-12-30T20:46:58Z The objective of this new paper was to evaluate the enzymatic esterification reaction conducted in supercritical or near-critical CO2, catalyzed by immobilized lipase B from Candida antarctica (CALB). The biocatalyst was prepared through the immobilization of CALB by covalent attachment using chitosan sequentially activated with Glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction (1: 1 (alcohol–acid); biocatalyst content, 10% (by substrate mass); 45 °C), an experimental design (23) was conducted to evaluate the effects of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum loading of chitosan was 20 mg protein/g support, and the thermal and solvent stability of the new biocatalyst was higher than that of the CALB-GX (by a 26-fold factor), CALB-OC (by a 53-fold factor) and Novozym 435 (by a 3-fold factor). The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 h. Additionally, the removal of water from the medium, by using molecular sieves, promoted a 16.0% increase in the conversion of oleic acid into ethyl esters. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Biotechnology Reports 14 16 26 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Lipase Immobilization Chitosan Fatty acid ethyl ester Esterification Supercritical fluids Biotechnology TP248.13-248.65 |
spellingShingle |
Lipase Immobilization Chitosan Fatty acid ethyl ester Esterification Supercritical fluids Biotechnology TP248.13-248.65 José C.S. dos Santos Horacio L. Bonazza Leonardo J.B.L. de Matos Elizabete A. Carneiro Oveimar Barbosa Roberto Fernandez-Lafuente Luciana R.B. Gonçalves Hosiberto B. de Sant’ Ana Rílvia S. Santiago-Aguiar Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
topic_facet |
Lipase Immobilization Chitosan Fatty acid ethyl ester Esterification Supercritical fluids Biotechnology TP248.13-248.65 |
description |
The objective of this new paper was to evaluate the enzymatic esterification reaction conducted in supercritical or near-critical CO2, catalyzed by immobilized lipase B from Candida antarctica (CALB). The biocatalyst was prepared through the immobilization of CALB by covalent attachment using chitosan sequentially activated with Glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction (1: 1 (alcohol–acid); biocatalyst content, 10% (by substrate mass); 45 °C), an experimental design (23) was conducted to evaluate the effects of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum loading of chitosan was 20 mg protein/g support, and the thermal and solvent stability of the new biocatalyst was higher than that of the CALB-GX (by a 26-fold factor), CALB-OC (by a 53-fold factor) and Novozym 435 (by a 3-fold factor). The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 h. Additionally, the removal of water from the medium, by using molecular sieves, promoted a 16.0% increase in the conversion of oleic acid into ethyl esters. |
format |
Article in Journal/Newspaper |
author |
José C.S. dos Santos Horacio L. Bonazza Leonardo J.B.L. de Matos Elizabete A. Carneiro Oveimar Barbosa Roberto Fernandez-Lafuente Luciana R.B. Gonçalves Hosiberto B. de Sant’ Ana Rílvia S. Santiago-Aguiar |
author_facet |
José C.S. dos Santos Horacio L. Bonazza Leonardo J.B.L. de Matos Elizabete A. Carneiro Oveimar Barbosa Roberto Fernandez-Lafuente Luciana R.B. Gonçalves Hosiberto B. de Sant’ Ana Rílvia S. Santiago-Aguiar |
author_sort |
José C.S. dos Santos |
title |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_short |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_full |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_fullStr |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_full_unstemmed |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_sort |
immobilization of calb on activated chitosan: application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.btre.2017.02.003 https://doaj.org/article/8360bd5898bb47a89ba6e37703b27ad0 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology Reports, Vol 14, Iss C, Pp 16-26 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2215017X16300911 https://doaj.org/toc/2215-017X 2215-017X doi:10.1016/j.btre.2017.02.003 https://doaj.org/article/8360bd5898bb47a89ba6e37703b27ad0 |
op_doi |
https://doi.org/10.1016/j.btre.2017.02.003 |
container_title |
Biotechnology Reports |
container_volume |
14 |
container_start_page |
16 |
op_container_end_page |
26 |
_version_ |
1766231963239186432 |