Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway.
Organophosphates (OP) are one of the major treatments used against the salmon louse (Lepeophtherius salmonis) in Norwegian salmonid aquaculture. The use of OP since the late 1970s has resulted in widespread resistant parasites. Recently, we reported a single mutation (Phe362Tyr) in acetylcholinester...
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ftdoajarticles:oai:doaj.org/article:82e42ad752d641879f38bfc826021e55 2023-05-15T16:13:42+02:00 Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. Kiranpreet Kaur Peder Andreas Jansen Vidar Teis Aspehaug Tor Einar Horsberg 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0149264 https://doaj.org/article/82e42ad752d641879f38bfc826021e55 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4755660?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0149264 https://doaj.org/article/82e42ad752d641879f38bfc826021e55 PLoS ONE, Vol 11, Iss 2, p e0149264 (2016) Medicine R Science Q article 2016 ftdoajarticles https://doi.org/10.1371/journal.pone.0149264 2022-12-31T07:26:52Z Organophosphates (OP) are one of the major treatments used against the salmon louse (Lepeophtherius salmonis) in Norwegian salmonid aquaculture. The use of OP since the late 1970s has resulted in widespread resistant parasites. Recently, we reported a single mutation (Phe362Tyr) in acetylcholinesterase (AChE) as the major mechanism behind resistance in salmon louse towards OP. The present study was carried out to validate this mechanism at the field level. A total of 6658 salmon louse samples were enrolled from 56 different fish farms across the Norwegian coast, from Vest Agder in the south to Finnmark in the north. All the samples were genotyped using a TaqMan probe assay for the Phe362Tyr mutation. A strong association was observed between areas with frequent use of the OP (azamethiphos) and the Phe362Tyr mutation. This was confirmed at 15 sites where results from independently conducted bioassays and genotyping of parasites correlated well. Furthermore, genotyping of surviving and moribund parasites from six bioassay experiments demonstrated a highly significant negative correlation between the frequency of resistance alleles and the probability of dying when exposed to azamethiphos in a bioassay. Based on these observations, we could strongly conclude that the Phe362Tyr mutation is a major factor responsible for OP resistance in salmon louse on Norwegian fish farms. Article in Journal/Newspaper Finnmark Finnmark Directory of Open Access Journals: DOAJ Articles Norway PLOS ONE 11 2 e0149264 |
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Medicine R Science Q Kiranpreet Kaur Peder Andreas Jansen Vidar Teis Aspehaug Tor Einar Horsberg Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. |
topic_facet |
Medicine R Science Q |
description |
Organophosphates (OP) are one of the major treatments used against the salmon louse (Lepeophtherius salmonis) in Norwegian salmonid aquaculture. The use of OP since the late 1970s has resulted in widespread resistant parasites. Recently, we reported a single mutation (Phe362Tyr) in acetylcholinesterase (AChE) as the major mechanism behind resistance in salmon louse towards OP. The present study was carried out to validate this mechanism at the field level. A total of 6658 salmon louse samples were enrolled from 56 different fish farms across the Norwegian coast, from Vest Agder in the south to Finnmark in the north. All the samples were genotyped using a TaqMan probe assay for the Phe362Tyr mutation. A strong association was observed between areas with frequent use of the OP (azamethiphos) and the Phe362Tyr mutation. This was confirmed at 15 sites where results from independently conducted bioassays and genotyping of parasites correlated well. Furthermore, genotyping of surviving and moribund parasites from six bioassay experiments demonstrated a highly significant negative correlation between the frequency of resistance alleles and the probability of dying when exposed to azamethiphos in a bioassay. Based on these observations, we could strongly conclude that the Phe362Tyr mutation is a major factor responsible for OP resistance in salmon louse on Norwegian fish farms. |
format |
Article in Journal/Newspaper |
author |
Kiranpreet Kaur Peder Andreas Jansen Vidar Teis Aspehaug Tor Einar Horsberg |
author_facet |
Kiranpreet Kaur Peder Andreas Jansen Vidar Teis Aspehaug Tor Einar Horsberg |
author_sort |
Kiranpreet Kaur |
title |
Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. |
title_short |
Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. |
title_full |
Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. |
title_fullStr |
Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. |
title_full_unstemmed |
Phe362Tyr in AChE: A Major Factor Responsible for Azamethiphos Resistance in Lepeophtheirus salmonis in Norway. |
title_sort |
phe362tyr in ache: a major factor responsible for azamethiphos resistance in lepeophtheirus salmonis in norway. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0149264 https://doaj.org/article/82e42ad752d641879f38bfc826021e55 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Finnmark Finnmark |
genre_facet |
Finnmark Finnmark |
op_source |
PLoS ONE, Vol 11, Iss 2, p e0149264 (2016) |
op_relation |
http://europepmc.org/articles/PMC4755660?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0149264 https://doaj.org/article/82e42ad752d641879f38bfc826021e55 |
op_doi |
https://doi.org/10.1371/journal.pone.0149264 |
container_title |
PLOS ONE |
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11 |
container_issue |
2 |
container_start_page |
e0149264 |
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1765999526504562688 |