Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology
Abstract Background Varestrongylus alces, a lungworm in Eurasian moose from Europe has been considered a junior synonym of Varestrongylus capreoli, in European roe deer, due to a poorly detailed morphological description and the absence of a type-series. Methods Specimens used in the redescription w...
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ftdoajarticles:oai:doaj.org/article:82b265c91dd74ab6997e19b55bbcc23f 2023-07-02T03:29:31+02:00 Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology Guilherme G Verocai Eric P Hoberg Turid Vikøren Kjell Handeland Bjørnar Ytrehus Andrew M Rezansoff Rebecca K Davidson John S Gilleard Susan J Kutz 2014-12-01T00:00:00Z https://doi.org/10.1186/s13071-014-0557-8 https://doaj.org/article/82b265c91dd74ab6997e19b55bbcc23f EN eng BMC https://doi.org/10.1186/s13071-014-0557-8 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-014-0557-8 1756-3305 https://doaj.org/article/82b265c91dd74ab6997e19b55bbcc23f Parasites & Vectors, Vol 7, Iss 1, Pp 1-21 (2014) Cervidae Cryptic species Historical biogeography ITS-2 Metastrongyloidea Parasite biodiversity Infectious and parasitic diseases RC109-216 article 2014 ftdoajarticles https://doi.org/10.1186/s13071-014-0557-8 2023-06-11T00:37:40Z Abstract Background Varestrongylus alces, a lungworm in Eurasian moose from Europe has been considered a junior synonym of Varestrongylus capreoli, in European roe deer, due to a poorly detailed morphological description and the absence of a type-series. Methods Specimens used in the redescription were collected from lesions in the lungs of Eurasian moose, from Vestby, Norway. Specimens were described based on comparative morphology and integrated approaches. Molecular identification was based on PCR, cloning and sequencing of the ITS-2 region of the nuclear ribosomal DNA. Phylogenetic analysis compared V. alces ITS-2 sequences to these of other Varestrongylus species and other protostrongylids. Results Varestrongylus alces is resurrected for protostrongylid nematodes of Eurasian moose from Europe. Varestrongylus alces causes firm nodular lesions that are clearly differentiated from the adjacent lung tissue. Histologically, lesions are restricted to the parenchyma with adult, egg and larval parasites surrounded by multinucleated giant cells, macrophages, eosinophilic granulocytes, lymphocytes. The species is valid and distinct from others referred to Varestrongylus, and should be separated from V. capreoli. Morphologically, V. alces can be distinguished from other species by characters in the males that include a distally bifurcated gubernaculum, arched denticulate crura, spicules that are equal in length and relatively short, and a dorsal ray that is elongate and bifurcated. Females have a well-developed provagina, and are very similar to those of V. capreoli. Morphometrics of first-stage larvae largely overlap with those of other Varestrongylus. Sequences of the ITS-2 region strongly support mutual independence of V. alces, V. cf. capreoli, and the yet undescribed species of Varestrongylus from North American ungulates. These three taxa form a well-supported crown-clade as the putative sister of V. alpenae. The association of V. alces and Alces or its ancestors is discussed in light of host and parasite ... Article in Journal/Newspaper Alces alces Directory of Open Access Journals: DOAJ Articles Norway Vestby ENVELOPE(19.336,19.336,69.017,69.017) Parasites & Vectors 7 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cervidae Cryptic species Historical biogeography ITS-2 Metastrongyloidea Parasite biodiversity Infectious and parasitic diseases RC109-216 |
spellingShingle |
Cervidae Cryptic species Historical biogeography ITS-2 Metastrongyloidea Parasite biodiversity Infectious and parasitic diseases RC109-216 Guilherme G Verocai Eric P Hoberg Turid Vikøren Kjell Handeland Bjørnar Ytrehus Andrew M Rezansoff Rebecca K Davidson John S Gilleard Susan J Kutz Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology |
topic_facet |
Cervidae Cryptic species Historical biogeography ITS-2 Metastrongyloidea Parasite biodiversity Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Varestrongylus alces, a lungworm in Eurasian moose from Europe has been considered a junior synonym of Varestrongylus capreoli, in European roe deer, due to a poorly detailed morphological description and the absence of a type-series. Methods Specimens used in the redescription were collected from lesions in the lungs of Eurasian moose, from Vestby, Norway. Specimens were described based on comparative morphology and integrated approaches. Molecular identification was based on PCR, cloning and sequencing of the ITS-2 region of the nuclear ribosomal DNA. Phylogenetic analysis compared V. alces ITS-2 sequences to these of other Varestrongylus species and other protostrongylids. Results Varestrongylus alces is resurrected for protostrongylid nematodes of Eurasian moose from Europe. Varestrongylus alces causes firm nodular lesions that are clearly differentiated from the adjacent lung tissue. Histologically, lesions are restricted to the parenchyma with adult, egg and larval parasites surrounded by multinucleated giant cells, macrophages, eosinophilic granulocytes, lymphocytes. The species is valid and distinct from others referred to Varestrongylus, and should be separated from V. capreoli. Morphologically, V. alces can be distinguished from other species by characters in the males that include a distally bifurcated gubernaculum, arched denticulate crura, spicules that are equal in length and relatively short, and a dorsal ray that is elongate and bifurcated. Females have a well-developed provagina, and are very similar to those of V. capreoli. Morphometrics of first-stage larvae largely overlap with those of other Varestrongylus. Sequences of the ITS-2 region strongly support mutual independence of V. alces, V. cf. capreoli, and the yet undescribed species of Varestrongylus from North American ungulates. These three taxa form a well-supported crown-clade as the putative sister of V. alpenae. The association of V. alces and Alces or its ancestors is discussed in light of host and parasite ... |
format |
Article in Journal/Newspaper |
author |
Guilherme G Verocai Eric P Hoberg Turid Vikøren Kjell Handeland Bjørnar Ytrehus Andrew M Rezansoff Rebecca K Davidson John S Gilleard Susan J Kutz |
author_facet |
Guilherme G Verocai Eric P Hoberg Turid Vikøren Kjell Handeland Bjørnar Ytrehus Andrew M Rezansoff Rebecca K Davidson John S Gilleard Susan J Kutz |
author_sort |
Guilherme G Verocai |
title |
Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology |
title_short |
Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology |
title_full |
Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology |
title_fullStr |
Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology |
title_full_unstemmed |
Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology |
title_sort |
resurrection and redescription of varestrongylus alces (nematoda: protostrongylidae), a lungworm of the eurasian moose (alces alces), with report on associated pathology |
publisher |
BMC |
publishDate |
2014 |
url |
https://doi.org/10.1186/s13071-014-0557-8 https://doaj.org/article/82b265c91dd74ab6997e19b55bbcc23f |
long_lat |
ENVELOPE(19.336,19.336,69.017,69.017) |
geographic |
Norway Vestby |
geographic_facet |
Norway Vestby |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
Parasites & Vectors, Vol 7, Iss 1, Pp 1-21 (2014) |
op_relation |
https://doi.org/10.1186/s13071-014-0557-8 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-014-0557-8 1756-3305 https://doaj.org/article/82b265c91dd74ab6997e19b55bbcc23f |
op_doi |
https://doi.org/10.1186/s13071-014-0557-8 |
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Parasites & Vectors |
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