Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections
Abstract Background As an opportunistic pathogen, Escherichia coli (E. coli) is widely recognized as the main cause of nosocomial infections as well as some disorders especially those associated with urinary tract infections (UTIs). This study, therefore, sets out to determine the extent of antibiot...
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ftdoajarticles:oai:doaj.org/article:8274438439ae4ef2a9b0d0da78179fe0 2023-05-15T15:14:59+02:00 Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections Ahmad FarajzadehSheikh Hojat Veisi Mojtaba Shahin Muhammad Getso Abbas Farahani 2019-03-01T00:00:00Z https://doi.org/10.1186/s41182-019-0147-8 https://doaj.org/article/8274438439ae4ef2a9b0d0da78179fe0 EN eng BMC http://link.springer.com/article/10.1186/s41182-019-0147-8 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-019-0147-8 1349-4147 https://doaj.org/article/8274438439ae4ef2a9b0d0da78179fe0 Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-7 (2019) E. coli UTIs Qnr ESBL Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.1186/s41182-019-0147-8 2022-12-31T14:46:29Z Abstract Background As an opportunistic pathogen, Escherichia coli (E. coli) is widely recognized as the main cause of nosocomial infections as well as some disorders especially those associated with urinary tract infections (UTIs). This study, therefore, sets out to determine the extent of antibiotic resistance to quinolones and to measure the frequency of qnr genes (A, B, and S) within extended-spectrum beta-lactamase (ESBL) and non-ESBL-producing strains of E. coli isolated from UTI-diagnosed patients as well as to investigate their antimicrobial susceptibility patterns for some selected antibiotics in southwest Iran. Methods Two hundred E. coli strains were isolated from UTI-diagnosed patients, hospitalized in nine different wards of Ahvaz Golestan Hospital between November 2015 and March 2016. The isolates were confirmed through well-practiced phenotypical methods. Moreover, the antimicrobial susceptibility test was successfully performed using a disk diffusion method. ESBL production among the isolates was screened by double disk synergism test (DDST), and the qnr genes were identified using a multiplex PCR. Results Out of the 200 samples collected, 167 isolates were confirmed to be E. coli strains. Maximum and minimum resistance were reported against nalidixic acid and chloramphenicol with 65.3% and 17.4%, respectively. Most of the isolates were resistant to all three types of quinolones studied in this research. Using multiplex PCR, the qnr genes were found in 100 (59.88%) strains (qnrA = 10, qnrB = 21, qnrS = 41, qnrB-S = 21, qnrB-A = 1, qnrA-S = 3, qnrA-B-S = 3), 58% of which was found among ESBL-producing isolates. Conclusions Resistance to quinolones antibiotics was highest among ESBL-producing isolates harboring, especially qnrS among other determinants of the qnr gene. There is a need for sensitive antibiotic stewardship especially in hospitals of Ahvaz, Khuzestan province. Further research is needed to ascertain the gravity of quinolones resistance in Iran and to quickly act against its spread ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 47 1 |
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English |
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E. coli UTIs Qnr ESBL Arctic medicine. Tropical medicine RC955-962 |
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E. coli UTIs Qnr ESBL Arctic medicine. Tropical medicine RC955-962 Ahmad FarajzadehSheikh Hojat Veisi Mojtaba Shahin Muhammad Getso Abbas Farahani Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections |
topic_facet |
E. coli UTIs Qnr ESBL Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background As an opportunistic pathogen, Escherichia coli (E. coli) is widely recognized as the main cause of nosocomial infections as well as some disorders especially those associated with urinary tract infections (UTIs). This study, therefore, sets out to determine the extent of antibiotic resistance to quinolones and to measure the frequency of qnr genes (A, B, and S) within extended-spectrum beta-lactamase (ESBL) and non-ESBL-producing strains of E. coli isolated from UTI-diagnosed patients as well as to investigate their antimicrobial susceptibility patterns for some selected antibiotics in southwest Iran. Methods Two hundred E. coli strains were isolated from UTI-diagnosed patients, hospitalized in nine different wards of Ahvaz Golestan Hospital between November 2015 and March 2016. The isolates were confirmed through well-practiced phenotypical methods. Moreover, the antimicrobial susceptibility test was successfully performed using a disk diffusion method. ESBL production among the isolates was screened by double disk synergism test (DDST), and the qnr genes were identified using a multiplex PCR. Results Out of the 200 samples collected, 167 isolates were confirmed to be E. coli strains. Maximum and minimum resistance were reported against nalidixic acid and chloramphenicol with 65.3% and 17.4%, respectively. Most of the isolates were resistant to all three types of quinolones studied in this research. Using multiplex PCR, the qnr genes were found in 100 (59.88%) strains (qnrA = 10, qnrB = 21, qnrS = 41, qnrB-S = 21, qnrB-A = 1, qnrA-S = 3, qnrA-B-S = 3), 58% of which was found among ESBL-producing isolates. Conclusions Resistance to quinolones antibiotics was highest among ESBL-producing isolates harboring, especially qnrS among other determinants of the qnr gene. There is a need for sensitive antibiotic stewardship especially in hospitals of Ahvaz, Khuzestan province. Further research is needed to ascertain the gravity of quinolones resistance in Iran and to quickly act against its spread ... |
format |
Article in Journal/Newspaper |
author |
Ahmad FarajzadehSheikh Hojat Veisi Mojtaba Shahin Muhammad Getso Abbas Farahani |
author_facet |
Ahmad FarajzadehSheikh Hojat Veisi Mojtaba Shahin Muhammad Getso Abbas Farahani |
author_sort |
Ahmad FarajzadehSheikh |
title |
Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections |
title_short |
Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections |
title_full |
Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections |
title_fullStr |
Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections |
title_full_unstemmed |
Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from urinary tract infections |
title_sort |
frequency of quinolone resistance genes among extended-spectrum β-lactamase (esbl)-producing escherichia coli strains isolated from urinary tract infections |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s41182-019-0147-8 https://doaj.org/article/8274438439ae4ef2a9b0d0da78179fe0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-7 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s41182-019-0147-8 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-019-0147-8 1349-4147 https://doaj.org/article/8274438439ae4ef2a9b0d0da78179fe0 |
op_doi |
https://doi.org/10.1186/s41182-019-0147-8 |
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Tropical Medicine and Health |
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47 |
container_issue |
1 |
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1766345372222881792 |