EAT BREATHE EXCRETE REPEAT: Physiological Responses of the Mussel Mytilus galloprovincialis to Diclofenac and Ocean Acidification

• Published in: Journal of Marine Science and Engineering
• Main Authors: Marco Munari, Valerio Matozzo, Verena Riedl, Paolo Pastore, Denis Badocco, Maria Gabriella Marin
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2020
• Subjects: ocean acidification; diclofenac; bivalves; clearance rate; respiration rate; excretion rate; Naval architecture. Shipbuilding. Marine engineering; VM1-989; Oceanography; GC1-1581; Ocean acidification
• Online Access: https://doi.org/10.3390/jmse8110907; https://doaj.org/article/8262273335d145018b4039ae9ffb3410

Combined effects of the nonsteroidal anti-inflammatory drug diclofenac and lowered seawater pH were assessed on the physiological responses of the mussel Mytilus galloprovincialis . Bivalves were exposed for 1 week to natural pH (8.1) and two reduced pH values (pH −0.4 units and pH −0.7 units), as predicted under a climate change scenario. After the first week, exposure continued for additional 2 weeks, both in the absence and in the presence of environmentally relevant concentrations of diclofenac (0.05 and 0.5 µg/L). Clearance rate, respiration rate, and excretion rate were measured after 7 days of exposure to pH only and after 14 (T1) and 21 (T2) days of exposure to the various pH*diclofenac combinations. At all sampling times, pH significantly affected all the biological parameters considered, whereas diclofenac generally exhibited a significant influence only at T2. Overall, results demonstrated that the physiological performance of M. galloprovincialis was strongly influenced by the experimental conditions tested, in particular by the interaction between the two stressors after 21 days of exposure. Further studies are needed to assess the combined effects of climate changes and emerging contaminants on bivalve physiology during different life stages, especially reproduction.