Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development
and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff –PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguis...
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2009
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ftdoajarticles:oai:doaj.org/article:823623ec3ebb4611bc04be2576077372 2023-05-15T18:19:30+02:00 Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development C Sarasquete E Gisbert L Ribeiro L Vieira Mt Dinis 2009-12-01T00:00:00Z https://doi.org/10.4081/1637 https://doaj.org/article/823623ec3ebb4611bc04be2576077372 EN eng PAGEPress Publications https://www.ejh.it/index.php/ejh/article/view/1637 https://doaj.org/toc/1121-760X https://doaj.org/toc/2038-8306 doi:10.4081/1637 1121-760X 2038-8306 https://doaj.org/article/823623ec3ebb4611bc04be2576077372 European Journal of Histochemistry, Vol 45, Iss 3 (2009) Biology (General) QH301-705.5 article 2009 ftdoajarticles https://doi.org/10.4081/1637 2022-12-31T01:44:14Z and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff –PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. Additionally, the presence and distribution of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated using horseradish peroxidase (HPR)-conjugated lectins (Con A, DBA, WGA and UEA-I). Most mucous cells (digestive, epidermal and branchial) of Siberian sturgeon, Acipenser baeri, sea bream, Sparus aurata and Senegal sole, Solea senegalensis larvae were PAS- and alcian blue- (pH 2.5 and 0.5) positive, with small variations between organs/tissues and species. Bromophenol blue reaction (general proteins) was positive in a minority of the mucous cells, usually in those cells which were PAS-negative. Proteins rich in sulphydryl (-SH) and/or disulphide (-S-S-) groups related with the glycoprotein nature of the glycoconjugates present in mucous cells were also observed. Epidermal, branchial and digestive mucous cells of all studied larvae did not contain glycogen or lipids. Article in Journal/Newspaper Siberian sturgeon Directory of Open Access Journals: DOAJ Articles European Journal of Histochemistry 45 3 267 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 C Sarasquete E Gisbert L Ribeiro L Vieira Mt Dinis Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
topic_facet |
Biology (General) QH301-705.5 |
description |
and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff –PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. Additionally, the presence and distribution of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated using horseradish peroxidase (HPR)-conjugated lectins (Con A, DBA, WGA and UEA-I). Most mucous cells (digestive, epidermal and branchial) of Siberian sturgeon, Acipenser baeri, sea bream, Sparus aurata and Senegal sole, Solea senegalensis larvae were PAS- and alcian blue- (pH 2.5 and 0.5) positive, with small variations between organs/tissues and species. Bromophenol blue reaction (general proteins) was positive in a minority of the mucous cells, usually in those cells which were PAS-negative. Proteins rich in sulphydryl (-SH) and/or disulphide (-S-S-) groups related with the glycoprotein nature of the glycoconjugates present in mucous cells were also observed. Epidermal, branchial and digestive mucous cells of all studied larvae did not contain glycogen or lipids. |
format |
Article in Journal/Newspaper |
author |
C Sarasquete E Gisbert L Ribeiro L Vieira Mt Dinis |
author_facet |
C Sarasquete E Gisbert L Ribeiro L Vieira Mt Dinis |
author_sort |
C Sarasquete |
title |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_short |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_full |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_fullStr |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_full_unstemmed |
Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development |
title_sort |
glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, sparus aurata, senegal sole, solea senegalensis and siberian sturgeon, acipenser baeri development |
publisher |
PAGEPress Publications |
publishDate |
2009 |
url |
https://doi.org/10.4081/1637 https://doaj.org/article/823623ec3ebb4611bc04be2576077372 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_source |
European Journal of Histochemistry, Vol 45, Iss 3 (2009) |
op_relation |
https://www.ejh.it/index.php/ejh/article/view/1637 https://doaj.org/toc/1121-760X https://doaj.org/toc/2038-8306 doi:10.4081/1637 1121-760X 2038-8306 https://doaj.org/article/823623ec3ebb4611bc04be2576077372 |
op_doi |
https://doi.org/10.4081/1637 |
container_title |
European Journal of Histochemistry |
container_volume |
45 |
container_issue |
3 |
container_start_page |
267 |
_version_ |
1766196587146510336 |