Pathogen surveillance in Southern Ocean pinnipeds

• Published in: Polar Research
• Main Authors: Sandra Núñez-Egido, Andrew Lowther, Ingebjørg H. Nymo, Jörn Klein, Eva M. Breines, Morten Tryland
• Format: Article in Journal/Newspaper
• Language: English
• Published: Norwegian Polar Institute 2020
• Subjects: antarctic fur seal; parapoxvirus; southern elephant seal; southern ocean; wildlife disease; zoonosis; Environmental sciences; GE1-350; Oceanography; GC1-1581; Antarctic; Southern Ocean; Austral; Bouvetøya; Antarc*; Antarctic Fur Seal; Antarctic Fur Seals; Arctocephalus gazella; Elephant Seal; Elephant Seals; Polar Research; Southern Elephant Seal; Southern Elephant Seals
• Online Access: https://doi.org/10.33265/polar.v39.3841; https://doaj.org/article/8234da1b9804463db00b3a7cf4810a55

Knowledge of the health status and potential effect of disease outbreaks among Southern Ocean fauna may be decisive for its conservation. We assessed the exposure and infection of Antarctic fur seals (Arctocephalus gazella, AFS) and Southern elephant seals (Mirounga leonine, SES) to parapoxvirus, Phocid alphaherpesvirus-1 (PhHV-1), smooth Brucella spp. and Toxoplasma gondii. AFS (n = 65) serum and swab samples, and SES (n = 13) serum samples from the sub--Antarctic island of Bouvetøya (54°25’S, 03°22’E) were collected during two austral summers (2014/15, 2017/18). Three polymerase chain reaction (PCR) tests amplifying the DNA polymerase, B2L and GIF parapoxvirus genomic regions were performed, investigating DNA from mucosal swab samples. The glycoprotein B gene was targeted to detect PhHV-1 viral DNA. Sera were assayed for T. gondii and smooth Brucella spp. antibodies with indirect enzyme-linked immunosorbent assays. Parapoxvirus PCR amplicons of the expected size were generated in two of the 29 AFS pups (nasal swabs, 2014/15), targeting the B2L (n = 2) and DNA polymerase (n = 1) genes, whereas the GIF PCR did not amplify target sequences. The PCR amplicons were sequenced and blasted in GenBank, revealing highest similarity with a seal parapoxvirus, confirming the presence of the virus in AFS for the first time. No PhHV-1 amplicons were generated, and antibodies against T. gondii or smooth Brucella spp. were not detected. Our data indicate that these seals are host for parapoxvirus but are neither exposed to smooth Brucella spp. nor T. gondii. Evidence of PhHV-1 shedding was not detected.