Comparative venomic profiles of three spiders of the genus Phoneutria
Background: Spider venoms induce different physio-pharmacological effects by binding with high affinity on molecular targets, therefore being of biotechnological interest. Some of these toxins, acting on different types of ion channels, have been identified in the venom of spiders of the genus Phone...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2021-0042 https://doaj.org/article/822d824ab6814526b2942143b42c3552 |
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ftdoajarticles:oai:doaj.org/article:822d824ab6814526b2942143b42c3552 2023-05-15T15:16:03+02:00 Comparative venomic profiles of three spiders of the genus Phoneutria Frederico Francisco Fernandes Juliana Rodrigues Moraes Jaqueline Leal dos Santos Thiago Geraldo Soares Vitor José Pinto Gouveia Alessandra C. S. Matavel William de Castro Borges Marta do Nascimento Cordeiro Suely Gomes Figueiredo Márcia Helena Borges 2022-02-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2021-0042 https://doaj.org/article/822d824ab6814526b2942143b42c3552 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992022000100302&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2021-0042 https://doaj.org/article/822d824ab6814526b2942143b42c3552 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 28 (2022) Spider venom Phoneutria Protein profile Toxins Ion channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2021-0042 2022-12-31T15:08:59Z Background: Spider venoms induce different physio-pharmacological effects by binding with high affinity on molecular targets, therefore being of biotechnological interest. Some of these toxins, acting on different types of ion channels, have been identified in the venom of spiders of the genus Phoneutria, mainly from P. nigriventer. In spite of the pharmaceutical potential demonstrated by P. nigriventer toxins, there is limited information on molecules from venoms of the same genus, as their toxins remain poorly characterized. Understanding this diversity and clarifying the differences in the mechanisms of action of spider toxins is of great importance for establishing their true biotechnological potential. This prompted us to compare three different venoms of the Phoneutria genus: P. nigriventer (Pn-V), P. eickstedtae (Pe-V) and P. pertyi (Pp-V). Methods: Biochemical and functional comparison of the venoms were carried out by SDS-PAGE, HPLC, mass spectrometry, enzymatic activities and electrophysiological assays (whole-cell patch clamp). Results: The employed approach revealed that all three venoms had an overall similarity in their components, with only minor differences. The presence of a high number of similar proteins was evident, particularly toxins in the mass range of ~6.0 kDa. Hyaluronidase and proteolytic activities were detected in all venoms, in addition to isoforms of the toxins Tx1 and Tx2-6. All Tx1 isoforms blocked Nav1.6 ion currents, with slight differences. Conclusion: Our findings showed that Pn-V, Pe-V and Pp-V are highly similar concerning protein composition and enzymatic activities, containing isoforms of the same toxins sharing high sequence homology, with minor modifications. However, these structural and functional variations are very important for venom diversity. In addition, our findings will contribute to the comprehension of the molecular diversity of the venoms of the other species from Phoneutria genus, exposing their biotechnological potential as a source for searching for new ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 28 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Spider venom Phoneutria Protein profile Toxins Ion channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Spider venom Phoneutria Protein profile Toxins Ion channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Frederico Francisco Fernandes Juliana Rodrigues Moraes Jaqueline Leal dos Santos Thiago Geraldo Soares Vitor José Pinto Gouveia Alessandra C. S. Matavel William de Castro Borges Marta do Nascimento Cordeiro Suely Gomes Figueiredo Márcia Helena Borges Comparative venomic profiles of three spiders of the genus Phoneutria |
topic_facet |
Spider venom Phoneutria Protein profile Toxins Ion channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background: Spider venoms induce different physio-pharmacological effects by binding with high affinity on molecular targets, therefore being of biotechnological interest. Some of these toxins, acting on different types of ion channels, have been identified in the venom of spiders of the genus Phoneutria, mainly from P. nigriventer. In spite of the pharmaceutical potential demonstrated by P. nigriventer toxins, there is limited information on molecules from venoms of the same genus, as their toxins remain poorly characterized. Understanding this diversity and clarifying the differences in the mechanisms of action of spider toxins is of great importance for establishing their true biotechnological potential. This prompted us to compare three different venoms of the Phoneutria genus: P. nigriventer (Pn-V), P. eickstedtae (Pe-V) and P. pertyi (Pp-V). Methods: Biochemical and functional comparison of the venoms were carried out by SDS-PAGE, HPLC, mass spectrometry, enzymatic activities and electrophysiological assays (whole-cell patch clamp). Results: The employed approach revealed that all three venoms had an overall similarity in their components, with only minor differences. The presence of a high number of similar proteins was evident, particularly toxins in the mass range of ~6.0 kDa. Hyaluronidase and proteolytic activities were detected in all venoms, in addition to isoforms of the toxins Tx1 and Tx2-6. All Tx1 isoforms blocked Nav1.6 ion currents, with slight differences. Conclusion: Our findings showed that Pn-V, Pe-V and Pp-V are highly similar concerning protein composition and enzymatic activities, containing isoforms of the same toxins sharing high sequence homology, with minor modifications. However, these structural and functional variations are very important for venom diversity. In addition, our findings will contribute to the comprehension of the molecular diversity of the venoms of the other species from Phoneutria genus, exposing their biotechnological potential as a source for searching for new ... |
format |
Article in Journal/Newspaper |
author |
Frederico Francisco Fernandes Juliana Rodrigues Moraes Jaqueline Leal dos Santos Thiago Geraldo Soares Vitor José Pinto Gouveia Alessandra C. S. Matavel William de Castro Borges Marta do Nascimento Cordeiro Suely Gomes Figueiredo Márcia Helena Borges |
author_facet |
Frederico Francisco Fernandes Juliana Rodrigues Moraes Jaqueline Leal dos Santos Thiago Geraldo Soares Vitor José Pinto Gouveia Alessandra C. S. Matavel William de Castro Borges Marta do Nascimento Cordeiro Suely Gomes Figueiredo Márcia Helena Borges |
author_sort |
Frederico Francisco Fernandes |
title |
Comparative venomic profiles of three spiders of the genus Phoneutria |
title_short |
Comparative venomic profiles of three spiders of the genus Phoneutria |
title_full |
Comparative venomic profiles of three spiders of the genus Phoneutria |
title_fullStr |
Comparative venomic profiles of three spiders of the genus Phoneutria |
title_full_unstemmed |
Comparative venomic profiles of three spiders of the genus Phoneutria |
title_sort |
comparative venomic profiles of three spiders of the genus phoneutria |
publisher |
SciELO |
publishDate |
2022 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2021-0042 https://doaj.org/article/822d824ab6814526b2942143b42c3552 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 28 (2022) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992022000100302&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2021-0042 https://doaj.org/article/822d824ab6814526b2942143b42c3552 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2021-0042 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
28 |
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1766346359840964608 |