Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein
Abstract Background As malaria incidence and transmission in a region decreases, it becomes increasingly difficult to identify areas of active transmission. Improved methods for identifying and monitoring foci of active malaria transmission are needed in areas of low parasite prevalence in order to...
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ftdoajarticles:oai:doaj.org/article:822cef6abd674fbba059e9f4ea253c1d 2023-05-15T15:18:05+02:00 Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein Jessica N. McCaffery Balwan Singh Douglas Nace Alberto Moreno Venkatachalam Udhayakumar Eric Rogier 2021-02-01T00:00:00Z https://doi.org/10.1186/s12936-021-03626-0 https://doaj.org/article/822cef6abd674fbba059e9f4ea253c1d EN eng BMC https://doi.org/10.1186/s12936-021-03626-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03626-0 1475-2875 https://doaj.org/article/822cef6abd674fbba059e9f4ea253c1d Malaria Journal, Vol 20, Iss 1, Pp 1-14 (2021) Malaria Plasmodium vivax Chimeric protein Serology Multiplex Seroepidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03626-0 2022-12-31T05:12:30Z Abstract Background As malaria incidence and transmission in a region decreases, it becomes increasingly difficult to identify areas of active transmission. Improved methods for identifying and monitoring foci of active malaria transmission are needed in areas of low parasite prevalence in order to achieve malaria elimination. Serological assays can provide population-level infection history to inform elimination campaigns. Methods A bead-based multiplex antibody detection assay was used to evaluate a chimeric Plasmodium vivax MSP1 protein (PvRMC-MSP1), designed to be broadly immunogenic for use in vaccine studies, to act as a pan-malaria serological tool based on its ability to capture IgG in plasma samples obtained from naturally exposed individuals. Samples from 236 US travellers with PCR confirmed infection status from all four major Plasmodium species infecting humans, Plasmodium falciparum (n = 181), Plasmodium vivax (n = 38), Plasmodium malariae (n = 4), and Plasmodium ovale (n = 13) were tested for IgG capture using PvRMC-MSP1 as well as the four recombinant MSP1-19 kD isoforms representative of these Plasmodium species. Results Regardless of infecting Plasmodium species, a large proportion of plasma samples from infected US travellers provided a high assay signal to the PvRMC-MSP1 chimeric protein, with 115 high responders out of 236 samples assessed (48.7%). When grouped by active infection, 38.7% P. falciparum-, 92.1% of P. vivax-, 75.0% P. malariae-, and 53.4% of P. ovale-infected individuals displayed high assay signals in response to PvRMC-MSP1. It was also determined that plasma from P. vivax-infected individuals produced increased assay signals in response to the PvRMC-MSP1 chimera as compared to the recombinant PvMSP1 for 89.5% (34 out of 38) of individuals. PvRMC-MSP1 also showed improved ability to capture IgG antibodies from P. falciparum-infected individuals when compared to the capture by recombinant PvMSP1, with high assay signals observed for 38.7% of P. falciparum-infected travellers in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Malaria Plasmodium vivax Chimeric protein Serology Multiplex Seroepidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Plasmodium vivax Chimeric protein Serology Multiplex Seroepidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jessica N. McCaffery Balwan Singh Douglas Nace Alberto Moreno Venkatachalam Udhayakumar Eric Rogier Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein |
topic_facet |
Malaria Plasmodium vivax Chimeric protein Serology Multiplex Seroepidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background As malaria incidence and transmission in a region decreases, it becomes increasingly difficult to identify areas of active transmission. Improved methods for identifying and monitoring foci of active malaria transmission are needed in areas of low parasite prevalence in order to achieve malaria elimination. Serological assays can provide population-level infection history to inform elimination campaigns. Methods A bead-based multiplex antibody detection assay was used to evaluate a chimeric Plasmodium vivax MSP1 protein (PvRMC-MSP1), designed to be broadly immunogenic for use in vaccine studies, to act as a pan-malaria serological tool based on its ability to capture IgG in plasma samples obtained from naturally exposed individuals. Samples from 236 US travellers with PCR confirmed infection status from all four major Plasmodium species infecting humans, Plasmodium falciparum (n = 181), Plasmodium vivax (n = 38), Plasmodium malariae (n = 4), and Plasmodium ovale (n = 13) were tested for IgG capture using PvRMC-MSP1 as well as the four recombinant MSP1-19 kD isoforms representative of these Plasmodium species. Results Regardless of infecting Plasmodium species, a large proportion of plasma samples from infected US travellers provided a high assay signal to the PvRMC-MSP1 chimeric protein, with 115 high responders out of 236 samples assessed (48.7%). When grouped by active infection, 38.7% P. falciparum-, 92.1% of P. vivax-, 75.0% P. malariae-, and 53.4% of P. ovale-infected individuals displayed high assay signals in response to PvRMC-MSP1. It was also determined that plasma from P. vivax-infected individuals produced increased assay signals in response to the PvRMC-MSP1 chimera as compared to the recombinant PvMSP1 for 89.5% (34 out of 38) of individuals. PvRMC-MSP1 also showed improved ability to capture IgG antibodies from P. falciparum-infected individuals when compared to the capture by recombinant PvMSP1, with high assay signals observed for 38.7% of P. falciparum-infected travellers in ... |
format |
Article in Journal/Newspaper |
author |
Jessica N. McCaffery Balwan Singh Douglas Nace Alberto Moreno Venkatachalam Udhayakumar Eric Rogier |
author_facet |
Jessica N. McCaffery Balwan Singh Douglas Nace Alberto Moreno Venkatachalam Udhayakumar Eric Rogier |
author_sort |
Jessica N. McCaffery |
title |
Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein |
title_short |
Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein |
title_full |
Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein |
title_fullStr |
Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein |
title_full_unstemmed |
Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein |
title_sort |
natural infections with different plasmodium species induce antibodies reactive to a chimeric plasmodium vivax recombinant protein |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03626-0 https://doaj.org/article/822cef6abd674fbba059e9f4ea253c1d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-14 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03626-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03626-0 1475-2875 https://doaj.org/article/822cef6abd674fbba059e9f4ea253c1d |
op_doi |
https://doi.org/10.1186/s12936-021-03626-0 |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766348320852148224 |