Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates
Abstract Background Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) and -2 (PfMSP-2) are major blood-stage vaccine candidate antigens. Understanding the genetic diversity of the genes, pfmsp-1 and pfmsp-2, is important for recognizing the genetic structure of P. falciparum, and the devel...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-019-2879-7 https://doaj.org/article/822c25411662425993955c429693dd8a |
id |
ftdoajarticles:oai:doaj.org/article:822c25411662425993955c429693dd8a |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:822c25411662425993955c429693dd8a 2023-05-15T15:19:05+02:00 Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates Hương Giang Lê Jung-Mi Kang Hojong Jun Jinyoung Lee Thị Lam Thái Moe Kyaw Myint Khin Saw Aye Woon-Mok Sohn Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na 2019-07-01T00:00:00Z https://doi.org/10.1186/s12936-019-2879-7 https://doaj.org/article/822c25411662425993955c429693dd8a EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2879-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2879-7 1475-2875 https://doaj.org/article/822c25411662425993955c429693dd8a Malaria Journal, Vol 18, Iss 1, Pp 1-13 (2019) Plasmodium falciparum Merozoite surface protein-1 Merozoite surface protein-2 Genetic diversity Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2879-7 2022-12-31T15:33:14Z Abstract Background Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) and -2 (PfMSP-2) are major blood-stage vaccine candidate antigens. Understanding the genetic diversity of the genes, pfmsp-1 and pfmsp-2, is important for recognizing the genetic structure of P. falciparum, and the development of an effective vaccine based on the antigens. In this study, the genetic diversities of pfmsp-1 and pfmsp-2 in the Myanmar P. falciparum were analysed. Methods The pfmsp-1 block 2 and pfmsp-2 block 3 regions were amplified by polymerase chain reaction from blood samples collected from Myanmar patients who were infected with P. falciparum in 2013–2015. The amplified gene fragments were cloned into a T&A vector, and sequenced. Sequence analysis of Myanmar pfmsp-1 block 2 and pfmsp-2 block 3 was performed to identify the genetic diversity of the regions. The temporal genetic changes of both pfmsp-1 and pfmsp-2 in the Myanmar P. falciparum population, as well as the polymorphic diversity in the publicly available global pfmsp-1 and pfmsp-2, were also comparatively analysed. Results High levels of genetic diversity of pfmsp-1 and pfmsp-2 were observed in the Myanmar P. falciparum isolates. Twenty-eight different alleles of pfmsp-1 (8 for K1 type, 14 for MAD20 type, and 6 for RO33 type) and 59 distinct alleles of pfmsp-2 (18 for FC27, and 41 for 3D7 type) were identified in the Myanmar P. falciparum population in amino acid level. Comparative analyses of the genetic diversity of the Myanmar pfmsp-1 and pfmsp-2 alleles in the recent (2013–2015) and past (2004–2006) Myanmar P. falciparum populations indicated the dynamic genetic expansion of the pfmsp-1 and pfmsp-2 in recent years, suggesting that a high level of genetic differentiation and recombination of the two genes may be maintained. Population genetic structure analysis of the global pfmsp-1 and pfmsp-2 also suggested that a high level of genetic diversity of the two genes was found in the global P. falciparum population. Conclusion Despite the recent ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Merozoite surface protein-1 Merozoite surface protein-2 Genetic diversity Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Merozoite surface protein-1 Merozoite surface protein-2 Genetic diversity Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Hương Giang Lê Jung-Mi Kang Hojong Jun Jinyoung Lee Thị Lam Thái Moe Kyaw Myint Khin Saw Aye Woon-Mok Sohn Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates |
topic_facet |
Plasmodium falciparum Merozoite surface protein-1 Merozoite surface protein-2 Genetic diversity Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) and -2 (PfMSP-2) are major blood-stage vaccine candidate antigens. Understanding the genetic diversity of the genes, pfmsp-1 and pfmsp-2, is important for recognizing the genetic structure of P. falciparum, and the development of an effective vaccine based on the antigens. In this study, the genetic diversities of pfmsp-1 and pfmsp-2 in the Myanmar P. falciparum were analysed. Methods The pfmsp-1 block 2 and pfmsp-2 block 3 regions were amplified by polymerase chain reaction from blood samples collected from Myanmar patients who were infected with P. falciparum in 2013–2015. The amplified gene fragments were cloned into a T&A vector, and sequenced. Sequence analysis of Myanmar pfmsp-1 block 2 and pfmsp-2 block 3 was performed to identify the genetic diversity of the regions. The temporal genetic changes of both pfmsp-1 and pfmsp-2 in the Myanmar P. falciparum population, as well as the polymorphic diversity in the publicly available global pfmsp-1 and pfmsp-2, were also comparatively analysed. Results High levels of genetic diversity of pfmsp-1 and pfmsp-2 were observed in the Myanmar P. falciparum isolates. Twenty-eight different alleles of pfmsp-1 (8 for K1 type, 14 for MAD20 type, and 6 for RO33 type) and 59 distinct alleles of pfmsp-2 (18 for FC27, and 41 for 3D7 type) were identified in the Myanmar P. falciparum population in amino acid level. Comparative analyses of the genetic diversity of the Myanmar pfmsp-1 and pfmsp-2 alleles in the recent (2013–2015) and past (2004–2006) Myanmar P. falciparum populations indicated the dynamic genetic expansion of the pfmsp-1 and pfmsp-2 in recent years, suggesting that a high level of genetic differentiation and recombination of the two genes may be maintained. Population genetic structure analysis of the global pfmsp-1 and pfmsp-2 also suggested that a high level of genetic diversity of the two genes was found in the global P. falciparum population. Conclusion Despite the recent ... |
format |
Article in Journal/Newspaper |
author |
Hương Giang Lê Jung-Mi Kang Hojong Jun Jinyoung Lee Thị Lam Thái Moe Kyaw Myint Khin Saw Aye Woon-Mok Sohn Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na |
author_facet |
Hương Giang Lê Jung-Mi Kang Hojong Jun Jinyoung Lee Thị Lam Thái Moe Kyaw Myint Khin Saw Aye Woon-Mok Sohn Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na |
author_sort |
Hương Giang Lê |
title |
Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates |
title_short |
Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates |
title_full |
Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates |
title_fullStr |
Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates |
title_full_unstemmed |
Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in Myanmar isolates |
title_sort |
changing pattern of the genetic diversities of plasmodium falciparum merozoite surface protein-1 and merozoite surface protein-2 in myanmar isolates |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2879-7 https://doaj.org/article/822c25411662425993955c429693dd8a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-13 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2879-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2879-7 1475-2875 https://doaj.org/article/822c25411662425993955c429693dd8a |
op_doi |
https://doi.org/10.1186/s12936-019-2879-7 |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766349273571524608 |