Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology
Abstract Background: The production of antivenom from immunized animals is an established treatment for snakebites; however, antibody phage display technology may have the capacity to delivery results more quickly and with a better match to local need. Naja oxiana, the Iranian cobra, is a medically...
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ftdoajarticles:oai:doaj.org/article:81f869e1f52d47449a4b24dc8a0c171c 2023-05-15T15:08:06+02:00 Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology Ali Nazari Maedeh Samianifard Hadi Rabie Abbas Zare Mirakabadi https://doi.org/10.1590/1678-9199-jvatitd-2019-0099 https://doaj.org/article/81f869e1f52d47449a4b24dc8a0c171c EN eng SciELO http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190099.pdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100317&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0099 https://doaj.org/article/81f869e1f52d47449a4b24dc8a0c171c Journal of Venomous Animals and Toxins including Tropical Diseases Antibody phage display Venom Antivenom Purification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0099 2022-12-31T16:21:55Z Abstract Background: The production of antivenom from immunized animals is an established treatment for snakebites; however, antibody phage display technology may have the capacity to delivery results more quickly and with a better match to local need. Naja oxiana, the Iranian cobra, is a medically important species, responsible for a significant number of deaths annually. This study was designed as proof of principle to determine whether recombinant antibodies with the capacity to neutralize cobra venom could be isolated by phage display. Methods: Toxic fractions from cobra venom were prepared by chromatography and used as targets in phage display to isolate recombinant antibodies from a human scFv library. Candidate antibodies were expressed in E. coli HB2151 and purified by IMAC chromatography. The selected clones were analyzed in in vivo and in vitro experiments. Results: Venom toxicity was contained in two fractions. Around a hundred phage clones were isolated against each fraction, those showing the best promise were G12F3 and G1F4. While all chosen clones showed low but detectable neutralizing effect against Naja oxiana venom, clone G12F3 could inhibit PLA2 activity. Conclusion: Therefore, phage display is believed to have a good potential as an approach to the development of snake antivenom. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Antibody phage display Venom Antivenom Purification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Antibody phage display Venom Antivenom Purification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Ali Nazari Maedeh Samianifard Hadi Rabie Abbas Zare Mirakabadi Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology |
topic_facet |
Antibody phage display Venom Antivenom Purification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: The production of antivenom from immunized animals is an established treatment for snakebites; however, antibody phage display technology may have the capacity to delivery results more quickly and with a better match to local need. Naja oxiana, the Iranian cobra, is a medically important species, responsible for a significant number of deaths annually. This study was designed as proof of principle to determine whether recombinant antibodies with the capacity to neutralize cobra venom could be isolated by phage display. Methods: Toxic fractions from cobra venom were prepared by chromatography and used as targets in phage display to isolate recombinant antibodies from a human scFv library. Candidate antibodies were expressed in E. coli HB2151 and purified by IMAC chromatography. The selected clones were analyzed in in vivo and in vitro experiments. Results: Venom toxicity was contained in two fractions. Around a hundred phage clones were isolated against each fraction, those showing the best promise were G12F3 and G1F4. While all chosen clones showed low but detectable neutralizing effect against Naja oxiana venom, clone G12F3 could inhibit PLA2 activity. Conclusion: Therefore, phage display is believed to have a good potential as an approach to the development of snake antivenom. |
format |
Article in Journal/Newspaper |
author |
Ali Nazari Maedeh Samianifard Hadi Rabie Abbas Zare Mirakabadi |
author_facet |
Ali Nazari Maedeh Samianifard Hadi Rabie Abbas Zare Mirakabadi |
author_sort |
Ali Nazari |
title |
Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology |
title_short |
Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology |
title_full |
Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology |
title_fullStr |
Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology |
title_full_unstemmed |
Recombinant antibodies against Iranian cobra venom as a new emerging therapy by phage display technology |
title_sort |
recombinant antibodies against iranian cobra venom as a new emerging therapy by phage display technology |
publisher |
SciELO |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0099 https://doaj.org/article/81f869e1f52d47449a4b24dc8a0c171c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases |
op_relation |
http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190099.pdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100317&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0099 https://doaj.org/article/81f869e1f52d47449a4b24dc8a0c171c |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0099 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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26 |
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1766339529727279104 |