Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles
ABSTRACT Escherichia coli causes various ailments such as septicemia, enteritis, foodborne illnesses, and urinary tract infections which are of concern in the public health field due to antibiotic resistance. Silver nanoparticles (AgNP) are known for their biocompatibility and antibacterial activity...
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ftdoajarticles:oai:doaj.org/article:81d57af0e77a4f04ac75ec3fed4da939 2024-09-09T19:27:03+00:00 Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles D'Andrea Ashmore Atul Chaudhari Brandi Barlow Brett Barlow Talia Harper Komal Vig Michael Miller Shree Singh Edward Nelson Shreekumar Pillai 2018-04-01T00:00:00Z https://doi.org/10.1590/s1678-9946201860018 https://doaj.org/article/81d57af0e77a4f04ac75ec3fed4da939 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652018005000209&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201860018 https://doaj.org/article/81d57af0e77a4f04ac75ec3fed4da939 Revista do Instituto de Medicina Tropical de São Paulo, Vol 60, Iss 0 (2018) Escherichia coli Polymer coated Nanosilver Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1590/s1678-9946201860018 2024-08-05T17:49:31Z ABSTRACT Escherichia coli causes various ailments such as septicemia, enteritis, foodborne illnesses, and urinary tract infections which are of concern in the public health field due to antibiotic resistance. Silver nanoparticles (AgNP) are known for their biocompatibility and antibacterial activity, and may prove to be an alternative method of treatment, especially as wound dressings. In this study, we compared the antibacterial efficacy of two polymer-coated silver nanoparticles either containing 10% Ag (Ag 10% + Polymer), or 99% Ag (AgPVP) in relation to plain uncoated silver nanoparticles (AgNP). Atomic force microscopy was used to characterize the nanoparticles, and their antibacterial efficacy was compared by the minimum inhibitory concentration (MIC) and bacterial growth curve assays, followed by molecular studies using scanning electron microscopy (SEM) and (qRT- PCR). AgNP inhibited the growth of E. coli only at 0.621 mg/mL, which was double the concentration required for both coated nanoparticles (0.312 mg/mL). Similarly, bacterial growth was impeded as early as 8 h at 0.156 mg/mL of both coated nanoparticles as compared to 0.312 mg/mL for plain AgNP. SEM data showed that nanoparticles damaged the cell membrane, resulting in bacterial cell lysis, expulsion of cellular contents, and complete disintegration of some cells. The expression of genes associated with the TCA cycle (aceF and frdB) and amino acid metabolism (gadB, metL, argC) were substantially downregulated in E. coli treated with nanoparticles. The reduction in the silver ion (Ag+) concentration of polymer-coated AgNP did not affect their antibacterial efficacy against E. coli. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 60 0 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Escherichia coli Polymer coated Nanosilver Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Escherichia coli Polymer coated Nanosilver Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 D'Andrea Ashmore Atul Chaudhari Brandi Barlow Brett Barlow Talia Harper Komal Vig Michael Miller Shree Singh Edward Nelson Shreekumar Pillai Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles |
topic_facet |
Escherichia coli Polymer coated Nanosilver Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Escherichia coli causes various ailments such as septicemia, enteritis, foodborne illnesses, and urinary tract infections which are of concern in the public health field due to antibiotic resistance. Silver nanoparticles (AgNP) are known for their biocompatibility and antibacterial activity, and may prove to be an alternative method of treatment, especially as wound dressings. In this study, we compared the antibacterial efficacy of two polymer-coated silver nanoparticles either containing 10% Ag (Ag 10% + Polymer), or 99% Ag (AgPVP) in relation to plain uncoated silver nanoparticles (AgNP). Atomic force microscopy was used to characterize the nanoparticles, and their antibacterial efficacy was compared by the minimum inhibitory concentration (MIC) and bacterial growth curve assays, followed by molecular studies using scanning electron microscopy (SEM) and (qRT- PCR). AgNP inhibited the growth of E. coli only at 0.621 mg/mL, which was double the concentration required for both coated nanoparticles (0.312 mg/mL). Similarly, bacterial growth was impeded as early as 8 h at 0.156 mg/mL of both coated nanoparticles as compared to 0.312 mg/mL for plain AgNP. SEM data showed that nanoparticles damaged the cell membrane, resulting in bacterial cell lysis, expulsion of cellular contents, and complete disintegration of some cells. The expression of genes associated with the TCA cycle (aceF and frdB) and amino acid metabolism (gadB, metL, argC) were substantially downregulated in E. coli treated with nanoparticles. The reduction in the silver ion (Ag+) concentration of polymer-coated AgNP did not affect their antibacterial efficacy against E. coli. |
format |
Article in Journal/Newspaper |
author |
D'Andrea Ashmore Atul Chaudhari Brandi Barlow Brett Barlow Talia Harper Komal Vig Michael Miller Shree Singh Edward Nelson Shreekumar Pillai |
author_facet |
D'Andrea Ashmore Atul Chaudhari Brandi Barlow Brett Barlow Talia Harper Komal Vig Michael Miller Shree Singh Edward Nelson Shreekumar Pillai |
author_sort |
D'Andrea Ashmore |
title |
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles |
title_short |
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles |
title_full |
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles |
title_fullStr |
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles |
title_full_unstemmed |
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles |
title_sort |
evaluation of e. coli inhibition by plain and polymer-coated silver nanoparticles |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2018 |
url |
https://doi.org/10.1590/s1678-9946201860018 https://doaj.org/article/81d57af0e77a4f04ac75ec3fed4da939 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 60, Iss 0 (2018) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652018005000209&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201860018 https://doaj.org/article/81d57af0e77a4f04ac75ec3fed4da939 |
op_doi |
https://doi.org/10.1590/s1678-9946201860018 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
60 |
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1809896560724541440 |