Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference?
The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique a...
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ftdoajarticles:oai:doaj.org/article:81bcfe8899094cfcae7cbbdd72affe43 2023-05-15T15:36:41+02:00 Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? Letizia Marsili Giovanni Di Guardo Sandro Mazzariol Silvia Casini 2019-05-01T00:00:00Z https://doi.org/10.3389/fimmu.2019.01219 https://doaj.org/article/81bcfe8899094cfcae7cbbdd72affe43 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2019.01219/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2019.01219 https://doaj.org/article/81bcfe8899094cfcae7cbbdd72affe43 Frontiers in Immunology, Vol 10 (2019) MICA cetaceans OCs PBDEs PAHs MeHg Immunologic diseases. Allergy RC581-607 article 2019 ftdoajarticles https://doi.org/10.3389/fimmu.2019.01219 2022-12-31T11:44:55Z The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique and to outline possible variations in MICA expression linked to different ecological and biological factors, while also investigating MICA expression after in vitro treatments with different contaminants. Free-ranging or stranded specimens of cetaceans were sampled in the Sea of Cortez (Mexico) (Balaenoptera edeni, Delphinus capensis, and Orcinus orca) and in the Mediterranean Sea (Balaenoptera physalus, Physeter macrocephalus, Tursiops truncatus, and Stenella coeruleoalba). Cell cultures were treated with an OC mixture, flame retardants, PAHs, MeHg, and BPA. The three species from the Sea of Cortez showed higher basal activity of MICA and lower levels of DDTs and PCBs than the Mediterranean species. A Pearson's linear coefficient equal to −0.45 also confirmed this tendency to have high levels of MICA and low total OC levels. Treatment of cultured fibroblasts with different contaminants mostly resulted in the upregulation of MICA protein expression by at least one treatment dose; downregulation was also found in some species or treatments. MICA alteration indicates a state of stress of the organism and a modification of the immune system's response and can be proposed as a non-invasive immunological marker that can be measured in skin biopsy samples, thus offering a good alternative to blood measurements. Article in Journal/Newspaper Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 10 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
MICA cetaceans OCs PBDEs PAHs MeHg Immunologic diseases. Allergy RC581-607 |
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MICA cetaceans OCs PBDEs PAHs MeHg Immunologic diseases. Allergy RC581-607 Letizia Marsili Giovanni Di Guardo Sandro Mazzariol Silvia Casini Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
topic_facet |
MICA cetaceans OCs PBDEs PAHs MeHg Immunologic diseases. Allergy RC581-607 |
description |
The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique and to outline possible variations in MICA expression linked to different ecological and biological factors, while also investigating MICA expression after in vitro treatments with different contaminants. Free-ranging or stranded specimens of cetaceans were sampled in the Sea of Cortez (Mexico) (Balaenoptera edeni, Delphinus capensis, and Orcinus orca) and in the Mediterranean Sea (Balaenoptera physalus, Physeter macrocephalus, Tursiops truncatus, and Stenella coeruleoalba). Cell cultures were treated with an OC mixture, flame retardants, PAHs, MeHg, and BPA. The three species from the Sea of Cortez showed higher basal activity of MICA and lower levels of DDTs and PCBs than the Mediterranean species. A Pearson's linear coefficient equal to −0.45 also confirmed this tendency to have high levels of MICA and low total OC levels. Treatment of cultured fibroblasts with different contaminants mostly resulted in the upregulation of MICA protein expression by at least one treatment dose; downregulation was also found in some species or treatments. MICA alteration indicates a state of stress of the organism and a modification of the immune system's response and can be proposed as a non-invasive immunological marker that can be measured in skin biopsy samples, thus offering a good alternative to blood measurements. |
format |
Article in Journal/Newspaper |
author |
Letizia Marsili Giovanni Di Guardo Sandro Mazzariol Silvia Casini |
author_facet |
Letizia Marsili Giovanni Di Guardo Sandro Mazzariol Silvia Casini |
author_sort |
Letizia Marsili |
title |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_short |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_full |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_fullStr |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_full_unstemmed |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_sort |
insights into cetacean immunology: do ecological and biological factors make the difference? |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fimmu.2019.01219 https://doaj.org/article/81bcfe8899094cfcae7cbbdd72affe43 |
genre |
Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus |
genre_facet |
Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus |
op_source |
Frontiers in Immunology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01219/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2019.01219 https://doaj.org/article/81bcfe8899094cfcae7cbbdd72affe43 |
op_doi |
https://doi.org/10.3389/fimmu.2019.01219 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
_version_ |
1766367042960621568 |