T-cell regulation in Erythema Nodosum Leprosum.
Leprosy is a disease caused by Mycobacterium leprae where the clinical spectrum correlates with the patient immune response. Erythema Nodosum Leprosum (ENL) is an immune-mediated inflammatory complication, which causes significant morbidity in affected leprosy patients. The underlying cause of ENL i...
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ftdoajarticles:oai:doaj.org/article:81b880265c5b4afdba6ba62f931e4af0 2023-05-15T15:15:22+02:00 T-cell regulation in Erythema Nodosum Leprosum. Edessa Negera Stephen L Walker Kidist Bobosha Rawleigh Howe Abraham Aseffa Hazel M Dockrell Diana N Lockwood 2017-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006001 https://doaj.org/article/81b880265c5b4afdba6ba62f931e4af0 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5648259?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006001 https://doaj.org/article/81b880265c5b4afdba6ba62f931e4af0 PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006001 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006001 2022-12-31T13:31:39Z Leprosy is a disease caused by Mycobacterium leprae where the clinical spectrum correlates with the patient immune response. Erythema Nodosum Leprosum (ENL) is an immune-mediated inflammatory complication, which causes significant morbidity in affected leprosy patients. The underlying cause of ENL is not conclusively known. However, immune-complexes and cell-mediated immunity have been suggested in the pathogenesis of ENL. The aim of this study was to investigate the regulatory T-cells in patients with ENL. Forty-six untreated patients with ENL and 31 non-reactional lepromatous leprosy (LL) patient controls visiting ALERT Hospital, Ethiopia were enrolled to the study. Blood samples were obtained before, during and after prednisolone treatment of ENL cases. Peripheral blood mononuclear cells (PBMCs) were isolated and used for immunophenotyping of regulatory T-cells by flow cytometry. Five markers: CD3, CD4 or CD8, CD25, CD27 and FoxP3 were used to define CD4+ and CD8+ regulatory T-cells. Clinical and histopathological data were obtained as supplementary information. All patients had been followed for 28 weeks. Patients with ENL reactions had a lower percentage of CD4+ regulatory T-cells (1.7%) than LL patient controls (3.8%) at diagnosis of ENL before treatment. After treatment, the percentage of CD4+regulatory T-cells was not significantly different between the two groups. The percentage of CD8+ regulatory T-cells was not significantly different in ENL and LL controls before and after treatment. Furthermore, patients with ENL had higher percentage of CD4+ T-ells and CD4+/CD8+ T-cells ratio than LL patient controls before treatment. The expression of CD25 on CD4+ and CD8+ T-cells was not significantly different in ENL and LL controls suggesting that CD25 expression is not associated with ENL reactions while FoxP3 expression on CD4+ T-cells was significantly lower in patients with ENL than in LL controls. We also found that prednisolone treatment of patients with ENL reactions suppresses CD4+ T-cell but not CD8+ ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 10 e0006001 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Edessa Negera Stephen L Walker Kidist Bobosha Rawleigh Howe Abraham Aseffa Hazel M Dockrell Diana N Lockwood T-cell regulation in Erythema Nodosum Leprosum. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leprosy is a disease caused by Mycobacterium leprae where the clinical spectrum correlates with the patient immune response. Erythema Nodosum Leprosum (ENL) is an immune-mediated inflammatory complication, which causes significant morbidity in affected leprosy patients. The underlying cause of ENL is not conclusively known. However, immune-complexes and cell-mediated immunity have been suggested in the pathogenesis of ENL. The aim of this study was to investigate the regulatory T-cells in patients with ENL. Forty-six untreated patients with ENL and 31 non-reactional lepromatous leprosy (LL) patient controls visiting ALERT Hospital, Ethiopia were enrolled to the study. Blood samples were obtained before, during and after prednisolone treatment of ENL cases. Peripheral blood mononuclear cells (PBMCs) were isolated and used for immunophenotyping of regulatory T-cells by flow cytometry. Five markers: CD3, CD4 or CD8, CD25, CD27 and FoxP3 were used to define CD4+ and CD8+ regulatory T-cells. Clinical and histopathological data were obtained as supplementary information. All patients had been followed for 28 weeks. Patients with ENL reactions had a lower percentage of CD4+ regulatory T-cells (1.7%) than LL patient controls (3.8%) at diagnosis of ENL before treatment. After treatment, the percentage of CD4+regulatory T-cells was not significantly different between the two groups. The percentage of CD8+ regulatory T-cells was not significantly different in ENL and LL controls before and after treatment. Furthermore, patients with ENL had higher percentage of CD4+ T-ells and CD4+/CD8+ T-cells ratio than LL patient controls before treatment. The expression of CD25 on CD4+ and CD8+ T-cells was not significantly different in ENL and LL controls suggesting that CD25 expression is not associated with ENL reactions while FoxP3 expression on CD4+ T-cells was significantly lower in patients with ENL than in LL controls. We also found that prednisolone treatment of patients with ENL reactions suppresses CD4+ T-cell but not CD8+ ... |
format |
Article in Journal/Newspaper |
author |
Edessa Negera Stephen L Walker Kidist Bobosha Rawleigh Howe Abraham Aseffa Hazel M Dockrell Diana N Lockwood |
author_facet |
Edessa Negera Stephen L Walker Kidist Bobosha Rawleigh Howe Abraham Aseffa Hazel M Dockrell Diana N Lockwood |
author_sort |
Edessa Negera |
title |
T-cell regulation in Erythema Nodosum Leprosum. |
title_short |
T-cell regulation in Erythema Nodosum Leprosum. |
title_full |
T-cell regulation in Erythema Nodosum Leprosum. |
title_fullStr |
T-cell regulation in Erythema Nodosum Leprosum. |
title_full_unstemmed |
T-cell regulation in Erythema Nodosum Leprosum. |
title_sort |
t-cell regulation in erythema nodosum leprosum. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006001 https://doaj.org/article/81b880265c5b4afdba6ba62f931e4af0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006001 (2017) |
op_relation |
http://europepmc.org/articles/PMC5648259?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006001 https://doaj.org/article/81b880265c5b4afdba6ba62f931e4af0 |
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https://doi.org/10.1371/journal.pntd.0006001 |
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PLOS Neglected Tropical Diseases |
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11 |
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10 |
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e0006001 |
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