Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia

Abstract Background Investigating the species distribution and their role in malaria transmission is important as it varies from place to place and is highly needed to design interventions appropriate to the site. The current study aimed to investigate the Anopheles mosquito species distribution and...

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Published in:Malaria Journal
Main Authors: Nigatu Eligo, Teklu Wegayehu, Myrthe Pareyn, Girum Tamiru, Bernt Lindtjørn, Fekadu Massebo
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2024
Subjects:
Online Access:https://doi.org/10.1186/s12936-024-04840-2
https://doaj.org/article/8168c79a5f6b4c57a57b1dc7f32a5293
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spelling ftdoajarticles:oai:doaj.org/article:8168c79a5f6b4c57a57b1dc7f32a5293 2024-02-11T10:01:49+01:00 Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia Nigatu Eligo Teklu Wegayehu Myrthe Pareyn Girum Tamiru Bernt Lindtjørn Fekadu Massebo 2024-01-01T00:00:00Z https://doi.org/10.1186/s12936-024-04840-2 https://doaj.org/article/8168c79a5f6b4c57a57b1dc7f32a5293 EN eng BMC https://doi.org/10.1186/s12936-024-04840-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-04840-2 1475-2875 https://doaj.org/article/8168c79a5f6b4c57a57b1dc7f32a5293 Malaria Journal, Vol 23, Iss 1, Pp 1-9 (2024) Anopheles arabiensis Anopheles sergentii Anopheles species distribution Circum-sporozoite proteins Ethiopia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2024 ftdoajarticles https://doi.org/10.1186/s12936-024-04840-2 2024-01-21T01:43:15Z Abstract Background Investigating the species distribution and their role in malaria transmission is important as it varies from place to place and is highly needed to design interventions appropriate to the site. The current study aimed to investigate the Anopheles mosquito species distribution and their infection rate in southwestern Ethiopia. Methods The study was conducted in 14 malaria-endemic kebeles (the smallest administrative unit), which were situated in eight different malaria-endemic districts and four zones in southwestern Ethiopia. Ten per cent of households in each village were visited to collect adult mosquitoes using Centers for Disease Control and Prevention (CDC) light traps. The larval and pupal collection was done from breeding sites within the villages, and reared to adults. Female mosquitoes were morphologically identified. The head and thorax of adult Anopheles mosquitoes were tested for circumsporozoite proteins (CSPs) using ELISA. At the same time, legs, wings, and abdomen were used to identify sibling species using PCR targeting the rDNA intergenic spacers region for species typing of the Anopheles funestus group and the internal transcribed spacer 2 region genes for Anopheles gambiae complex. Results A total of 1445 Anopheles mosquitoes comprising eight species were collected. Of 813 An. gambiae complex tested by PCR, 785 (97%) were Anopheles arabiensis, and the remaining 28 (3%) were not amplified. There were 133 An. funestus group captured and tested to identify the species, of which 117 (88%) were positive for Anopheles parensis, and 15 (11%) were not amplified. A single specimen (1%) showed a band with a different base pair length from the known An. funestus group species. Sequencing revealed this was Anopheles sergentii. Among 1399 Anopheles tested for CSPs by ELISA, 5 (0.4%) An. arabiensis were positive for Plasmodium falciparum and a single (0.07%) was positive for Plasmodium vivax. Conclusions Anopheles arabiensis continues to play the principal role in malaria transmission ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 23 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Anopheles arabiensis
Anopheles sergentii
Anopheles species distribution
Circum-sporozoite proteins
Ethiopia
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Anopheles arabiensis
Anopheles sergentii
Anopheles species distribution
Circum-sporozoite proteins
Ethiopia
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Nigatu Eligo
Teklu Wegayehu
Myrthe Pareyn
Girum Tamiru
Bernt Lindtjørn
Fekadu Massebo
Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia
topic_facet Anopheles arabiensis
Anopheles sergentii
Anopheles species distribution
Circum-sporozoite proteins
Ethiopia
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Investigating the species distribution and their role in malaria transmission is important as it varies from place to place and is highly needed to design interventions appropriate to the site. The current study aimed to investigate the Anopheles mosquito species distribution and their infection rate in southwestern Ethiopia. Methods The study was conducted in 14 malaria-endemic kebeles (the smallest administrative unit), which were situated in eight different malaria-endemic districts and four zones in southwestern Ethiopia. Ten per cent of households in each village were visited to collect adult mosquitoes using Centers for Disease Control and Prevention (CDC) light traps. The larval and pupal collection was done from breeding sites within the villages, and reared to adults. Female mosquitoes were morphologically identified. The head and thorax of adult Anopheles mosquitoes were tested for circumsporozoite proteins (CSPs) using ELISA. At the same time, legs, wings, and abdomen were used to identify sibling species using PCR targeting the rDNA intergenic spacers region for species typing of the Anopheles funestus group and the internal transcribed spacer 2 region genes for Anopheles gambiae complex. Results A total of 1445 Anopheles mosquitoes comprising eight species were collected. Of 813 An. gambiae complex tested by PCR, 785 (97%) were Anopheles arabiensis, and the remaining 28 (3%) were not amplified. There were 133 An. funestus group captured and tested to identify the species, of which 117 (88%) were positive for Anopheles parensis, and 15 (11%) were not amplified. A single specimen (1%) showed a band with a different base pair length from the known An. funestus group species. Sequencing revealed this was Anopheles sergentii. Among 1399 Anopheles tested for CSPs by ELISA, 5 (0.4%) An. arabiensis were positive for Plasmodium falciparum and a single (0.07%) was positive for Plasmodium vivax. Conclusions Anopheles arabiensis continues to play the principal role in malaria transmission ...
format Article in Journal/Newspaper
author Nigatu Eligo
Teklu Wegayehu
Myrthe Pareyn
Girum Tamiru
Bernt Lindtjørn
Fekadu Massebo
author_facet Nigatu Eligo
Teklu Wegayehu
Myrthe Pareyn
Girum Tamiru
Bernt Lindtjørn
Fekadu Massebo
author_sort Nigatu Eligo
title Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia
title_short Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia
title_full Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia
title_fullStr Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia
title_full_unstemmed Anopheles arabiensis continues to be the primary vector of Plasmodium falciparum after decades of malaria control in southwestern Ethiopia
title_sort anopheles arabiensis continues to be the primary vector of plasmodium falciparum after decades of malaria control in southwestern ethiopia
publisher BMC
publishDate 2024
url https://doi.org/10.1186/s12936-024-04840-2
https://doaj.org/article/8168c79a5f6b4c57a57b1dc7f32a5293
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 23, Iss 1, Pp 1-9 (2024)
op_relation https://doi.org/10.1186/s12936-024-04840-2
https://doaj.org/toc/1475-2875
doi:10.1186/s12936-024-04840-2
1475-2875
https://doaj.org/article/8168c79a5f6b4c57a57b1dc7f32a5293
op_doi https://doi.org/10.1186/s12936-024-04840-2
container_title Malaria Journal
container_volume 23
container_issue 1
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