A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.
BACKGROUND:Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counte...
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ftdoajarticles:oai:doaj.org/article:815d7ada8d9043179ba08081f0891b9c 2023-05-15T15:05:17+02:00 A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana 2016-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004581 https://doaj.org/article/815d7ada8d9043179ba08081f0891b9c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4851354?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004581 https://doaj.org/article/815d7ada8d9043179ba08081f0891b9c PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004581 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004581 2022-12-31T05:43:56Z BACKGROUND:Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses. METHODS/PRINCIPAL FINDINGS:Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. CONCLUSIONS/SIGNIFICANCE:The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 4 e0004581 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses. METHODS/PRINCIPAL FINDINGS:Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. CONCLUSIONS/SIGNIFICANCE:The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. |
format |
Article in Journal/Newspaper |
author |
Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana |
author_facet |
Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana |
author_sort |
Paula Beatriz Santiago |
title |
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
title_short |
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
title_full |
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
title_fullStr |
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
title_full_unstemmed |
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
title_sort |
deep insight into the sialome of rhodnius neglectus, a vector of chagas disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004581 https://doaj.org/article/815d7ada8d9043179ba08081f0891b9c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004581 (2016) |
op_relation |
http://europepmc.org/articles/PMC4851354?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004581 https://doaj.org/article/815d7ada8d9043179ba08081f0891b9c |
op_doi |
https://doi.org/10.1371/journal.pntd.0004581 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
4 |
container_start_page |
e0004581 |
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1766337013461549056 |