Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae
The subject of this study was the development of midbrain in beluga sturgeon (Huso huso) larvae. To this end, 36 larvae (6 larvae of each age) were fixed in 10% buffered formalin, dehydrated in an etha-nol series, cleared in xylene and embedded in paraffin. The larvae brain were very small in size,...
Published in: | Bulgarian Journal of Veterinary Medicine |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2018
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ftdoajarticles:oai:doaj.org/article:81328f97a1de4766ace13eb94505405c 2023-05-15T15:41:47+02:00 Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae S. Tavighi Z. Saadatfar B. Shojaei 2018-03-01T00:00:00Z https://doi.org/10.15547/bjvm.1035 https://doaj.org/article/81328f97a1de4766ace13eb94505405c EN eng Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria http://tru.uni-sz.bg/bjvm/BJVM-March%202018%20p.17-27.pdf https://doaj.org/toc/1311-1477 https://doaj.org/toc/1313-3543 doi:10.15547/bjvm.1035 1311-1477 1313-3543 https://doaj.org/article/81328f97a1de4766ace13eb94505405c Bulgarian Journal of Veterinary Medicine, Vol 21, Iss 1, Pp 17-27 (2018) development Huso huso larva midbrain Veterinary medicine SF600-1100 article 2018 ftdoajarticles https://doi.org/10.15547/bjvm.1035 2022-12-31T10:08:51Z The subject of this study was the development of midbrain in beluga sturgeon (Huso huso) larvae. To this end, 36 larvae (6 larvae of each age) were fixed in 10% buffered formalin, dehydrated in an etha-nol series, cleared in xylene and embedded in paraffin. The larvae brain were very small in size, total head was fixed and sited in paraffin blocks. Serial sections (6 µm thick) were stained with haematoxy-lin & eosin, Luxol fast blue, cresyl echt violet and PTAH Mallory for histological studies by light microscope. A stereological study was done by the Cavalieri principle to estimate the different areas of the midbrain, about 10 sections from each specimen were selected and photographed by a camera attached to the light microscope Olympus CX22. From 1 day of age, the two parts of midbrain, optic tectum and tegmentum were obvious. There was an area between these parts called Torus semicircu-laris. In 6-day-old larvae, the optic tectum cell layers were partially observed and in 54-day-olds, the optic tectum occupied the largest volume of midbrain (12.16±0.07 mm3). Tectal ventricle in H. huso larvae was observed from the first day. The tegmentum was located in the ventral part of the midbrain and in its dorsal part joined the hindbrain and contained some nuclei. Stereological results showed significantly distinctive regional differences in midbrain volume (P<0.05). In conclusion, according to the present research, the evolution of midbrain in H. huso was related to ecological activities and in comparison with other ray-finned fishes was not very developed. Article in Journal/Newspaper Beluga Beluga* Directory of Open Access Journals: DOAJ Articles Olympus ENVELOPE(156.767,156.767,-80.217,-80.217) Bulgarian Journal of Veterinary Medicine 21 1 17 27 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
development Huso huso larva midbrain Veterinary medicine SF600-1100 |
spellingShingle |
development Huso huso larva midbrain Veterinary medicine SF600-1100 S. Tavighi Z. Saadatfar B. Shojaei Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae |
topic_facet |
development Huso huso larva midbrain Veterinary medicine SF600-1100 |
description |
The subject of this study was the development of midbrain in beluga sturgeon (Huso huso) larvae. To this end, 36 larvae (6 larvae of each age) were fixed in 10% buffered formalin, dehydrated in an etha-nol series, cleared in xylene and embedded in paraffin. The larvae brain were very small in size, total head was fixed and sited in paraffin blocks. Serial sections (6 µm thick) were stained with haematoxy-lin & eosin, Luxol fast blue, cresyl echt violet and PTAH Mallory for histological studies by light microscope. A stereological study was done by the Cavalieri principle to estimate the different areas of the midbrain, about 10 sections from each specimen were selected and photographed by a camera attached to the light microscope Olympus CX22. From 1 day of age, the two parts of midbrain, optic tectum and tegmentum were obvious. There was an area between these parts called Torus semicircu-laris. In 6-day-old larvae, the optic tectum cell layers were partially observed and in 54-day-olds, the optic tectum occupied the largest volume of midbrain (12.16±0.07 mm3). Tectal ventricle in H. huso larvae was observed from the first day. The tegmentum was located in the ventral part of the midbrain and in its dorsal part joined the hindbrain and contained some nuclei. Stereological results showed significantly distinctive regional differences in midbrain volume (P<0.05). In conclusion, according to the present research, the evolution of midbrain in H. huso was related to ecological activities and in comparison with other ray-finned fishes was not very developed. |
format |
Article in Journal/Newspaper |
author |
S. Tavighi Z. Saadatfar B. Shojaei |
author_facet |
S. Tavighi Z. Saadatfar B. Shojaei |
author_sort |
S. Tavighi |
title |
Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae |
title_short |
Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae |
title_full |
Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae |
title_fullStr |
Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae |
title_full_unstemmed |
Midbrain morphogenesis in beluga sturgeon (Huso huso) larvae |
title_sort |
midbrain morphogenesis in beluga sturgeon (huso huso) larvae |
publisher |
Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
publishDate |
2018 |
url |
https://doi.org/10.15547/bjvm.1035 https://doaj.org/article/81328f97a1de4766ace13eb94505405c |
long_lat |
ENVELOPE(156.767,156.767,-80.217,-80.217) |
geographic |
Olympus |
geographic_facet |
Olympus |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Bulgarian Journal of Veterinary Medicine, Vol 21, Iss 1, Pp 17-27 (2018) |
op_relation |
http://tru.uni-sz.bg/bjvm/BJVM-March%202018%20p.17-27.pdf https://doaj.org/toc/1311-1477 https://doaj.org/toc/1313-3543 doi:10.15547/bjvm.1035 1311-1477 1313-3543 https://doaj.org/article/81328f97a1de4766ace13eb94505405c |
op_doi |
https://doi.org/10.15547/bjvm.1035 |
container_title |
Bulgarian Journal of Veterinary Medicine |
container_volume |
21 |
container_issue |
1 |
container_start_page |
17 |
op_container_end_page |
27 |
_version_ |
1766374681260064768 |