Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for el...
Published in: | Royal Society Open Science |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
The Royal Society
2016
|
Subjects: | |
Online Access: | https://doi.org/10.1098/rsos.160106 https://doaj.org/article/80ee18881baf4eed9b71acde4d944153 |
id |
ftdoajarticles:oai:doaj.org/article:80ee18881baf4eed9b71acde4d944153 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:80ee18881baf4eed9b71acde4d944153 2023-05-15T13:46:02+02:00 Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis Masaharu Tsuji 2016-01-01T00:00:00Z https://doi.org/10.1098/rsos.160106 https://doaj.org/article/80ee18881baf4eed9b71acde4d944153 EN eng The Royal Society https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106 https://doaj.org/toc/2054-5703 2054-5703 doi:10.1098/rsos.160106 https://doaj.org/article/80ee18881baf4eed9b71acde4d944153 Royal Society Open Science, Vol 3, Iss 7 (2016) cold stress basidiomycetous yeast metabolite response capillary electrophoresis–time-of-flight mass spectrometry mrakia blollopis Science Q article 2016 ftdoajarticles https://doi.org/10.1098/rsos.160106 2022-12-31T10:00:28Z Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Royal Society Open Science 3 7 160106 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
cold stress basidiomycetous yeast metabolite response capillary electrophoresis–time-of-flight mass spectrometry mrakia blollopis Science Q |
spellingShingle |
cold stress basidiomycetous yeast metabolite response capillary electrophoresis–time-of-flight mass spectrometry mrakia blollopis Science Q Masaharu Tsuji Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
topic_facet |
cold stress basidiomycetous yeast metabolite response capillary electrophoresis–time-of-flight mass spectrometry mrakia blollopis Science Q |
description |
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. |
format |
Article in Journal/Newspaper |
author |
Masaharu Tsuji |
author_facet |
Masaharu Tsuji |
author_sort |
Masaharu Tsuji |
title |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_short |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_fullStr |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full_unstemmed |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_sort |
cold-stress responses in the antarctic basidiomycetous yeast mrakia blollopis |
publisher |
The Royal Society |
publishDate |
2016 |
url |
https://doi.org/10.1098/rsos.160106 https://doaj.org/article/80ee18881baf4eed9b71acde4d944153 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Royal Society Open Science, Vol 3, Iss 7 (2016) |
op_relation |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106 https://doaj.org/toc/2054-5703 2054-5703 doi:10.1098/rsos.160106 https://doaj.org/article/80ee18881baf4eed9b71acde4d944153 |
op_doi |
https://doi.org/10.1098/rsos.160106 |
container_title |
Royal Society Open Science |
container_volume |
3 |
container_issue |
7 |
container_start_page |
160106 |
_version_ |
1766235233275871232 |