Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection Sonda marcada com digoxigenina para a detecção do gene N do vírus da raiva

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Pedro Carnieli Junior, Armando Moraes Ventura, Edison Luiz Durigon
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2006
• Subjects: Raiva; Gene N; Transcrição reversa-reação em cadeia da polimerase; Sonda; Hibridação; Rabies; N Gene; Reverse transcription polymerase chain reaction; Probe; Hybridization; Arctic medicine. Tropical medicine; RC955-962; Arctic; Pasteur
• Online Access: https://doi.org/10.1590/S0037-86822006000200005; https://doaj.org/article/80d7965cd8d64f0ead9f212fd6510b1f

A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreeme...