Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes
A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s...
Published in: | Italian Journal of Animal Science |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Taylor & Francis Group
2010
|
Subjects: | |
Online Access: | https://doi.org/10.4081/ijas.2007.1s.161 https://doaj.org/article/806fd5a7d4ab4694b6e45e59a8263008 |
id |
ftdoajarticles:oai:doaj.org/article:806fd5a7d4ab4694b6e45e59a8263008 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:806fd5a7d4ab4694b6e45e59a8263008 2023-05-15T15:27:08+02:00 Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes R. Mantovani G. Barcaccia C. Dalvit E. Reffo F. Maretto 2010-04-01T00:00:00Z https://doi.org/10.4081/ijas.2007.1s.161 https://doaj.org/article/806fd5a7d4ab4694b6e45e59a8263008 EN eng Taylor & Francis Group http://www.aspajournal.it/index.php/ijas/article/view/1427 https://doaj.org/toc/1594-4077 https://doaj.org/toc/1828-051X doi:10.4081/ijas.2007.1s.161 1594-4077 1828-051X https://doaj.org/article/806fd5a7d4ab4694b6e45e59a8263008 Italian Journal of Animal Science, Vol 6, Iss 1s, Pp 161-163 (2010) Genetic traceability Gadiformes SNPs 16S rRNA Animal culture SF1-1100 article 2010 ftdoajarticles https://doi.org/10.4081/ijas.2007.1s.161 2022-12-31T06:29:44Z A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry. Article in Journal/Newspaper atlantic cod Gadus morhua Directory of Open Access Journals: DOAJ Articles Pacific Italian Journal of Animal Science 6 sup1 161 163 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Genetic traceability Gadiformes SNPs 16S rRNA Animal culture SF1-1100 |
spellingShingle |
Genetic traceability Gadiformes SNPs 16S rRNA Animal culture SF1-1100 R. Mantovani G. Barcaccia C. Dalvit E. Reffo F. Maretto Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
topic_facet |
Genetic traceability Gadiformes SNPs 16S rRNA Animal culture SF1-1100 |
description |
A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry. |
format |
Article in Journal/Newspaper |
author |
R. Mantovani G. Barcaccia C. Dalvit E. Reffo F. Maretto |
author_facet |
R. Mantovani G. Barcaccia C. Dalvit E. Reffo F. Maretto |
author_sort |
R. Mantovani |
title |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_short |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_full |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_fullStr |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_full_unstemmed |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_sort |
finding 16s rrna gene-based snps for the genetic traceability of commercial species belonging to gadiformes |
publisher |
Taylor & Francis Group |
publishDate |
2010 |
url |
https://doi.org/10.4081/ijas.2007.1s.161 https://doaj.org/article/806fd5a7d4ab4694b6e45e59a8263008 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Italian Journal of Animal Science, Vol 6, Iss 1s, Pp 161-163 (2010) |
op_relation |
http://www.aspajournal.it/index.php/ijas/article/view/1427 https://doaj.org/toc/1594-4077 https://doaj.org/toc/1828-051X doi:10.4081/ijas.2007.1s.161 1594-4077 1828-051X https://doaj.org/article/806fd5a7d4ab4694b6e45e59a8263008 |
op_doi |
https://doi.org/10.4081/ijas.2007.1s.161 |
container_title |
Italian Journal of Animal Science |
container_volume |
6 |
container_issue |
sup1 |
container_start_page |
161 |
op_container_end_page |
163 |
_version_ |
1766357594388037632 |