Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus.
Orientia tsutsugamushi is the causal agent of scrub typhus, a public health problem in the Asia-Pacific region and a life-threatening disease. O. tsutsugamushi is an obligate intracellular bacterium that mainly infects endothelial cells. We demonstrated here that O. tsutsugamushi also replicated in...
Published in: | PLoS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:8066bab7e10341e9a27cb5e384fe72da 2023-05-15T15:13:00+02:00 Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. Wiwit Tantibhedhyangkul Thanavadee Prachason Duangdao Waywa Adil El Filali Eric Ghigo Wanna Thongnoppakhun Didier Raoult Yupin Suputtamongkol Christian Capo Chanin Limwongse Jean-Louis Mege 2011-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001028 https://doaj.org/article/8066bab7e10341e9a27cb5e384fe72da EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3096591?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001028 https://doaj.org/article/8066bab7e10341e9a27cb5e384fe72da PLoS Neglected Tropical Diseases, Vol 5, Iss 5, p e1028 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001028 2022-12-31T15:00:44Z Orientia tsutsugamushi is the causal agent of scrub typhus, a public health problem in the Asia-Pacific region and a life-threatening disease. O. tsutsugamushi is an obligate intracellular bacterium that mainly infects endothelial cells. We demonstrated here that O. tsutsugamushi also replicated in monocytes isolated from healthy donors. In addition, O. tsutsugamushi altered the expression of more than 4,500 genes, as demonstrated by microarray analysis. The expression of type I interferon, interferon-stimulated genes and genes associated with the M1 polarization of macrophages was significantly upregulated. O. tsutsugamushi also induced the expression of apoptosis-related genes and promoted cell death in a small percentage of monocytes. Live organisms were indispensable to the type I interferon response and apoptosis and enhanced the expression of M1-associated cytokines. These data were related to the transcriptional changes detected in mononuclear cells isolated from patients with scrub typhus. Here, the microarray analyses revealed the upregulation of 613 genes, which included interferon-related genes, and some features of M1 polarization were observed in these patients, similar to what was observed in O. tsutsugamushi-stimulated monocytes in vitro. This is the first report demonstrating that monocytes are clearly polarized in vitro and ex vivo following exposure to O. tsutsugamushi. These results would improve our understanding of the pathogenesis of scrub typhus, during which interferon-mediated activation of monocytes and their subsequent polarization into an M1 phenotype appear critical. This study may give us a clue of new tools for the diagnosis of patients with scrub typhus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pacific PLoS Neglected Tropical Diseases 5 5 e1028 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Wiwit Tantibhedhyangkul Thanavadee Prachason Duangdao Waywa Adil El Filali Eric Ghigo Wanna Thongnoppakhun Didier Raoult Yupin Suputtamongkol Christian Capo Chanin Limwongse Jean-Louis Mege Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Orientia tsutsugamushi is the causal agent of scrub typhus, a public health problem in the Asia-Pacific region and a life-threatening disease. O. tsutsugamushi is an obligate intracellular bacterium that mainly infects endothelial cells. We demonstrated here that O. tsutsugamushi also replicated in monocytes isolated from healthy donors. In addition, O. tsutsugamushi altered the expression of more than 4,500 genes, as demonstrated by microarray analysis. The expression of type I interferon, interferon-stimulated genes and genes associated with the M1 polarization of macrophages was significantly upregulated. O. tsutsugamushi also induced the expression of apoptosis-related genes and promoted cell death in a small percentage of monocytes. Live organisms were indispensable to the type I interferon response and apoptosis and enhanced the expression of M1-associated cytokines. These data were related to the transcriptional changes detected in mononuclear cells isolated from patients with scrub typhus. Here, the microarray analyses revealed the upregulation of 613 genes, which included interferon-related genes, and some features of M1 polarization were observed in these patients, similar to what was observed in O. tsutsugamushi-stimulated monocytes in vitro. This is the first report demonstrating that monocytes are clearly polarized in vitro and ex vivo following exposure to O. tsutsugamushi. These results would improve our understanding of the pathogenesis of scrub typhus, during which interferon-mediated activation of monocytes and their subsequent polarization into an M1 phenotype appear critical. This study may give us a clue of new tools for the diagnosis of patients with scrub typhus. |
format |
Article in Journal/Newspaper |
author |
Wiwit Tantibhedhyangkul Thanavadee Prachason Duangdao Waywa Adil El Filali Eric Ghigo Wanna Thongnoppakhun Didier Raoult Yupin Suputtamongkol Christian Capo Chanin Limwongse Jean-Louis Mege |
author_facet |
Wiwit Tantibhedhyangkul Thanavadee Prachason Duangdao Waywa Adil El Filali Eric Ghigo Wanna Thongnoppakhun Didier Raoult Yupin Suputtamongkol Christian Capo Chanin Limwongse Jean-Louis Mege |
author_sort |
Wiwit Tantibhedhyangkul |
title |
Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
title_short |
Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
title_full |
Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
title_fullStr |
Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
title_full_unstemmed |
Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
title_sort |
orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001028 https://doaj.org/article/8066bab7e10341e9a27cb5e384fe72da |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 5, p e1028 (2011) |
op_relation |
http://europepmc.org/articles/PMC3096591?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001028 https://doaj.org/article/8066bab7e10341e9a27cb5e384fe72da |
op_doi |
https://doi.org/10.1371/journal.pntd.0001028 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
5 |
container_start_page |
e1028 |
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1766343599703719936 |