Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
Dingkundan is a traditional Chinese medicine formula consisted of 30 herbs, such as Radix ginseng Rubra, Cervus nippon Temminck, Saffron Stigma Croci and Pseudo-ginseng. The quality control of Cervi cornu Pantotrichum is of important for maintaining the effectiveness of Dingkundan. Herein, a method...
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Editorial Board of Journal of Chinese Mass Spectrometry Society
2023
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Online Access: | https://doi.org/10.7538/zpxb.2023.0011 https://doaj.org/article/803c74c3674b46bb93177a49789c7139 |
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ftdoajarticles:oai:doaj.org/article:803c74c3674b46bb93177a49789c7139 2024-02-11T10:08:10+01:00 Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry 2023-09-01T00:00:00Z https://doi.org/10.7538/zpxb.2023.0011 https://doaj.org/article/803c74c3674b46bb93177a49789c7139 EN ZH eng chi Editorial Board of Journal of Chinese Mass Spectrometry Society http://www.jcmss.com.cn/CN/PDF/10.7538/zpxb.2023.0011?token=bba041b5331d41cfa0d724be17ad61aa https://doaj.org/toc/1004-2997 doi:10.7538/zpxb.2023.0011 1004-2997 https://doaj.org/article/803c74c3674b46bb93177a49789c7139 Zhipu Xuebao, Vol 44, Iss 5, Pp 624-634 (2023) ultra-high performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms) dingkundan cervi cornu pantotrichum rangifer tarandus linnaeus characteristic peptides Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.7538/zpxb.2023.0011 2024-01-14T01:48:00Z Dingkundan is a traditional Chinese medicine formula consisted of 30 herbs, such as Radix ginseng Rubra, Cervus nippon Temminck, Saffron Stigma Croci and Pseudo-ginseng. The quality control of Cervi cornu Pantotrichum is of important for maintaining the effectiveness of Dingkundan. Herein, a method of ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for specificity identification of the Cervi Cornu Pantotrichum and Rangifer tarandus Linnaeus in Dingkundan. Dingkundan samples were digested by trypsin, and the tryptic peptides were subsequently separated by using Acquity UPLC BEH C18 column (2.1 mm×100 mm×1.7 μm) with the mobile phases comprising of acetonitrile-containing 0.1% formic acid (A) and water containing 0.1% formic acid (B). The gradient started with 3%A, and increased to 8%A at 10 min, to 20%A at 20 min, to 50%A at 30 min with a flowing rate of 0.3 mL/min. The injection volume was 5 μL. The electrospray ionization (ESI+) MS analysis was performed under positive ion mode with multiple reaction monitoring (MRM) mode. The specificity of each monitoring ion pairs of Cervi Cornu Pantotrichum and Rangifer tarandus Linnaeus were well distinguished and characterized. All 30 batches of Dingkundan samples were detected with components originated from Pantotrichum, 8 batches were detected with components from Cervi Cornu Pantotrichum, and the other 22 batches were detected with ingredients from Rangifer tarandus Linnaeus, indicating more ingredients from pantotrichum in Dingkundan in present markets comes from Rangifer tarandus Linnaeus. The UPLC-MS/MS method has good specificity, features with simple and quick operation, further improving the quality standard of Dingkundan and providing reference for the identification of Cervi Cornu Pantotrichum and screening of Rangifer tarandus Linnaeus in traditional Chinese medicine formulae containing Cervi Cornu Pantotrichum. Article in Journal/Newspaper Rangifer tarandus Directory of Open Access Journals: DOAJ Articles Cornu ENVELOPE(-60.628,-60.628,-64.168,-64.168) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Chinese |
topic |
ultra-high performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms) dingkundan cervi cornu pantotrichum rangifer tarandus linnaeus characteristic peptides Chemistry QD1-999 |
spellingShingle |
ultra-high performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms) dingkundan cervi cornu pantotrichum rangifer tarandus linnaeus characteristic peptides Chemistry QD1-999 Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
topic_facet |
ultra-high performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms) dingkundan cervi cornu pantotrichum rangifer tarandus linnaeus characteristic peptides Chemistry QD1-999 |
description |
Dingkundan is a traditional Chinese medicine formula consisted of 30 herbs, such as Radix ginseng Rubra, Cervus nippon Temminck, Saffron Stigma Croci and Pseudo-ginseng. The quality control of Cervi cornu Pantotrichum is of important for maintaining the effectiveness of Dingkundan. Herein, a method of ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for specificity identification of the Cervi Cornu Pantotrichum and Rangifer tarandus Linnaeus in Dingkundan. Dingkundan samples were digested by trypsin, and the tryptic peptides were subsequently separated by using Acquity UPLC BEH C18 column (2.1 mm×100 mm×1.7 μm) with the mobile phases comprising of acetonitrile-containing 0.1% formic acid (A) and water containing 0.1% formic acid (B). The gradient started with 3%A, and increased to 8%A at 10 min, to 20%A at 20 min, to 50%A at 30 min with a flowing rate of 0.3 mL/min. The injection volume was 5 μL. The electrospray ionization (ESI+) MS analysis was performed under positive ion mode with multiple reaction monitoring (MRM) mode. The specificity of each monitoring ion pairs of Cervi Cornu Pantotrichum and Rangifer tarandus Linnaeus were well distinguished and characterized. All 30 batches of Dingkundan samples were detected with components originated from Pantotrichum, 8 batches were detected with components from Cervi Cornu Pantotrichum, and the other 22 batches were detected with ingredients from Rangifer tarandus Linnaeus, indicating more ingredients from pantotrichum in Dingkundan in present markets comes from Rangifer tarandus Linnaeus. The UPLC-MS/MS method has good specificity, features with simple and quick operation, further improving the quality standard of Dingkundan and providing reference for the identification of Cervi Cornu Pantotrichum and screening of Rangifer tarandus Linnaeus in traditional Chinese medicine formulae containing Cervi Cornu Pantotrichum. |
format |
Article in Journal/Newspaper |
title |
Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_short |
Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_full |
Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_fullStr |
Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_full_unstemmed |
Specificity Identification of Cervi Cornu Pantotrichum and Screening of Rangifer tarandus Linnaeus in Dingkundan by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_sort |
specificity identification of cervi cornu pantotrichum and screening of rangifer tarandus linnaeus in dingkundan by ultra-high performance liquid chromatography-tandem mass spectrometry |
publisher |
Editorial Board of Journal of Chinese Mass Spectrometry Society |
publishDate |
2023 |
url |
https://doi.org/10.7538/zpxb.2023.0011 https://doaj.org/article/803c74c3674b46bb93177a49789c7139 |
long_lat |
ENVELOPE(-60.628,-60.628,-64.168,-64.168) |
geographic |
Cornu |
geographic_facet |
Cornu |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Zhipu Xuebao, Vol 44, Iss 5, Pp 624-634 (2023) |
op_relation |
http://www.jcmss.com.cn/CN/PDF/10.7538/zpxb.2023.0011?token=bba041b5331d41cfa0d724be17ad61aa https://doaj.org/toc/1004-2997 doi:10.7538/zpxb.2023.0011 1004-2997 https://doaj.org/article/803c74c3674b46bb93177a49789c7139 |
op_doi |
https://doi.org/10.7538/zpxb.2023.0011 |
_version_ |
1790607161651363840 |