Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China
Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan...
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Tehran University of Medical Sciences
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ftdoajarticles:oai:doaj.org/article:8026b3a3b98942208ee62737fe46da5f 2023-05-15T18:05:23+02:00 Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China XinLei WANG Ling DONG Li ZHANG Yan LV HaiLong LI 2019-03-01T00:00:00Z https://doaj.org/article/8026b3a3b98942208ee62737fe46da5f EN eng Tehran University of Medical Sciences http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1755 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X 1735-7020 2008-238X https://doaj.org/article/8026b3a3b98942208ee62737fe46da5f Iranian Journal of Parasitology, Vol 14, Iss 1, Pp 106-110 (2019) Toxoplasma gondii Genetic typing PCR-RFLP Wild rodents China Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles 2022-12-31T05:27:21Z Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China. Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles |
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topic |
Toxoplasma gondii Genetic typing PCR-RFLP Wild rodents China Infectious and parasitic diseases RC109-216 |
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Toxoplasma gondii Genetic typing PCR-RFLP Wild rodents China Infectious and parasitic diseases RC109-216 XinLei WANG Ling DONG Li ZHANG Yan LV HaiLong LI Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China |
topic_facet |
Toxoplasma gondii Genetic typing PCR-RFLP Wild rodents China Infectious and parasitic diseases RC109-216 |
description |
Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China. |
format |
Article in Journal/Newspaper |
author |
XinLei WANG Ling DONG Li ZHANG Yan LV HaiLong LI |
author_facet |
XinLei WANG Ling DONG Li ZHANG Yan LV HaiLong LI |
author_sort |
XinLei WANG |
title |
Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China |
title_short |
Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China |
title_full |
Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China |
title_fullStr |
Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China |
title_full_unstemmed |
Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China |
title_sort |
genetic characterization of toxoplasma gondii from wild rodents in sichuan province, southwestern china |
publisher |
Tehran University of Medical Sciences |
publishDate |
2019 |
url |
https://doaj.org/article/8026b3a3b98942208ee62737fe46da5f |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Iranian Journal of Parasitology, Vol 14, Iss 1, Pp 106-110 (2019) |
op_relation |
http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1755 https://doaj.org/toc/1735-7020 https://doaj.org/toc/2008-238X 1735-7020 2008-238X https://doaj.org/article/8026b3a3b98942208ee62737fe46da5f |
_version_ |
1766176852762689536 |