Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.)
Frequently, diseases in aquaculture have been fought indiscriminately with the use of antibiotics, which has led to the development and dissemination of (multiple) antibiotic resistances in bacteria. Consequently, it is necessary to look for alternative and complementary approaches to chemotheraphy...
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MDPI AG
2024
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Online Access: | https://doi.org/10.3390/vetsci11080365 https://doaj.org/article/7ff0d8123d6c49f9a3c7b6d9ac52ae29 |
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ftdoajarticles:oai:doaj.org/article:7ff0d8123d6c49f9a3c7b6d9ac52ae29 2024-09-15T18:25:28+00:00 Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) Lara Díaz-Formoso Diogo Contente Javier Feito Pablo E. Hernández Juan Borrero Estefanía Muñoz-Atienza Luis M. Cintas 2024-08-01T00:00:00Z https://doi.org/10.3390/vetsci11080365 https://doaj.org/article/7ff0d8123d6c49f9a3c7b6d9ac52ae29 EN eng MDPI AG https://www.mdpi.com/2306-7381/11/8/365 https://doaj.org/toc/2306-7381 doi:10.3390/vetsci11080365 2306-7381 https://doaj.org/article/7ff0d8123d6c49f9a3c7b6d9ac52ae29 Veterinary Sciences, Vol 11, Iss 8, p 365 (2024) marine fish probiotics lactic acid bacteria (LAB) whole-genome sequencing (WGS) antimicrobial peptides Veterinary medicine SF600-1100 article 2024 ftdoajarticles https://doi.org/10.3390/vetsci11080365 2024-09-02T15:34:37Z Frequently, diseases in aquaculture have been fought indiscriminately with the use of antibiotics, which has led to the development and dissemination of (multiple) antibiotic resistances in bacteria. Consequently, it is necessary to look for alternative and complementary approaches to chemotheraphy that are safe for humans, animals, and the environment, such as the use of probiotics in fish farming. The objective of this work was the Whole-Genome Sequencing (WGS) and bioinformatic and functional analyses of S. salivarius MDI13 and L. sakei MEI5, two LAB strains isolated from the gut of commercial European hakes ( M. merluccius , L.) caught in the Northeast Atlantic Ocean. The WGS and bioinformatic and functional analyses confirmed the lack of transferable antibiotic resistance genes, the lack of virulence and pathogenicity issues, and their potentially probiotic characteristics. Specifically, genes involved in adhesion and aggregation, vitamin biosynthesis, and amino acid metabolism were detected in both strains. In addition, genes related to lactic acid production, active metabolism, and/or adaptation to stress and adverse conditions in the host gastrointestinal tract were detected in L. sakei MEI5. Moreover, a gene cluster encoding three bacteriocins (SlvV, BlpK, and BlpE) was identified in the genome of S. salivarius MDI13. The in vitro-synthesized bacteriocin BlpK showed antimicrobial activity against the ichthyopathogens Lc. garvieae and S. parauberis . Altogether, our results suggest that S. salivarius MDI13 and L. sakei MEI5 have a strong potential as probiotics to prevent fish diseases in aquaculture as an appropriate alternative/complementary strategy to the use of antibiotics. Article in Journal/Newspaper Northeast Atlantic Directory of Open Access Journals: DOAJ Articles Veterinary Sciences 11 8 365 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
marine fish probiotics lactic acid bacteria (LAB) whole-genome sequencing (WGS) antimicrobial peptides Veterinary medicine SF600-1100 |
spellingShingle |
marine fish probiotics lactic acid bacteria (LAB) whole-genome sequencing (WGS) antimicrobial peptides Veterinary medicine SF600-1100 Lara Díaz-Formoso Diogo Contente Javier Feito Pablo E. Hernández Juan Borrero Estefanía Muñoz-Atienza Luis M. Cintas Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) |
topic_facet |
marine fish probiotics lactic acid bacteria (LAB) whole-genome sequencing (WGS) antimicrobial peptides Veterinary medicine SF600-1100 |
description |
Frequently, diseases in aquaculture have been fought indiscriminately with the use of antibiotics, which has led to the development and dissemination of (multiple) antibiotic resistances in bacteria. Consequently, it is necessary to look for alternative and complementary approaches to chemotheraphy that are safe for humans, animals, and the environment, such as the use of probiotics in fish farming. The objective of this work was the Whole-Genome Sequencing (WGS) and bioinformatic and functional analyses of S. salivarius MDI13 and L. sakei MEI5, two LAB strains isolated from the gut of commercial European hakes ( M. merluccius , L.) caught in the Northeast Atlantic Ocean. The WGS and bioinformatic and functional analyses confirmed the lack of transferable antibiotic resistance genes, the lack of virulence and pathogenicity issues, and their potentially probiotic characteristics. Specifically, genes involved in adhesion and aggregation, vitamin biosynthesis, and amino acid metabolism were detected in both strains. In addition, genes related to lactic acid production, active metabolism, and/or adaptation to stress and adverse conditions in the host gastrointestinal tract were detected in L. sakei MEI5. Moreover, a gene cluster encoding three bacteriocins (SlvV, BlpK, and BlpE) was identified in the genome of S. salivarius MDI13. The in vitro-synthesized bacteriocin BlpK showed antimicrobial activity against the ichthyopathogens Lc. garvieae and S. parauberis . Altogether, our results suggest that S. salivarius MDI13 and L. sakei MEI5 have a strong potential as probiotics to prevent fish diseases in aquaculture as an appropriate alternative/complementary strategy to the use of antibiotics. |
format |
Article in Journal/Newspaper |
author |
Lara Díaz-Formoso Diogo Contente Javier Feito Pablo E. Hernández Juan Borrero Estefanía Muñoz-Atienza Luis M. Cintas |
author_facet |
Lara Díaz-Formoso Diogo Contente Javier Feito Pablo E. Hernández Juan Borrero Estefanía Muñoz-Atienza Luis M. Cintas |
author_sort |
Lara Díaz-Formoso |
title |
Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) |
title_short |
Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) |
title_full |
Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) |
title_fullStr |
Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) |
title_full_unstemmed |
Genomic Sequence of Streptococcus salivarius MDI13 and Latilactobacillus sakei MEI5: Two Promising Probiotic Strains Isolated from European Hakes ( Merluccius merluccius , L.) |
title_sort |
genomic sequence of streptococcus salivarius mdi13 and latilactobacillus sakei mei5: two promising probiotic strains isolated from european hakes ( merluccius merluccius , l.) |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://doi.org/10.3390/vetsci11080365 https://doaj.org/article/7ff0d8123d6c49f9a3c7b6d9ac52ae29 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Veterinary Sciences, Vol 11, Iss 8, p 365 (2024) |
op_relation |
https://www.mdpi.com/2306-7381/11/8/365 https://doaj.org/toc/2306-7381 doi:10.3390/vetsci11080365 2306-7381 https://doaj.org/article/7ff0d8123d6c49f9a3c7b6d9ac52ae29 |
op_doi |
https://doi.org/10.3390/vetsci11080365 |
container_title |
Veterinary Sciences |
container_volume |
11 |
container_issue |
8 |
container_start_page |
365 |
_version_ |
1810465979376861184 |