Antisickling activity of butyl stearate isolated from Ocimum basilicum (Lamiaceae)

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Dorothée Dinangayi Tshilanda, Pius Tshimankinda Mpiana, Damase Nguwo Vele Onyamboko, Blaise Mavinga Mbala, Koto-te-Nyiwa Ngbolua, Damien Sha Tshibey Tshibangu, Matthieu Kokengo Bokolo, Kalulu Muzele Taba, Teddy Kabeya Kasonga
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: Sickle cell disease; Antisickling plant; Ocimum basilicum; Butyl stearate; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic; Sickle
• Online Access: https://doi.org/10.12980/APJTB.4.2014C1329; https://doaj.org/article/7fa260d848fb4948bd914e19566a769f

Objective: To perform phytochemical analyses on the leaves of Ocimum basilicum L. (O. basilicum), to elucidate the structure of isolate and then perform the antisickling activity on the crude extract and on the isolate. Methods: The Emmel test performed on the acidified methanolic extract of this plant was used to evaluate the antisickling activity. The structure characterization of the active compound was performed using chromatographic techniques for the separation and the spectroscopic ones for structure elucidation (1H-NMR, 13C-NMR, COSY, HMBC). Results: The chemical screening on the crude extract revealed the presence of polyphenols (flavonoids, anthocyanins, leucoanthocyanins, tannins, quinones) alkaloids, saponins, triterpenoids and steroids. The obtained extract after evaporation yielded 34.50 g (11.5%) out of 300 g of powdered leaves of O. basilicum. The acidified methanolic extract and butyl stearate showed an interesting antisickling activity. Conclusions: The acidified methanolic extract and butyl stearate from O. basilicum displayed a good antisickling activity. To the best of our knowledge, this is the first time to report the antisickling activity of this compound in this plant. The synthesized compound presented the same spectroscopic characteristics than the natural one and the antisickling activities of its derivatives are understudying.