Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis
The genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified...
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ftdoajarticles:oai:doaj.org/article:7f9cc35f56214c8fba9da79ed3dca1a9 2023-05-15T13:31:08+02:00 Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh 2022-01-01T00:00:00Z https://doi.org/10.1155/2022/1162938 https://doaj.org/article/7f9cc35f56214c8fba9da79ed3dca1a9 EN eng Hindawi Limited http://dx.doi.org/10.1155/2022/1162938 https://doaj.org/toc/1687-9198 1687-9198 doi:10.1155/2022/1162938 https://doaj.org/article/7f9cc35f56214c8fba9da79ed3dca1a9 International Journal of Microbiology, Vol 2022 (2022) Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.1155/2022/1162938 2022-12-30T20:38:08Z The genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified a species of Arthrobacter isolated from seawater in the polar region using 16S rRNA sequence analysis. The strain PAMC25284 genome consists of a circular chromosome with a GC content of 65.6% and is projected to contain 3,588 genes, of which 3,150 are protein coding, 366 are pseudogenes, 19 are rRNA coding, and 50 are tRNA coding genes. Using comparative genomics, we showed that PMAC25284 has copper-transporting ATPases, copper chaperone, copper-responsive transcriptional regulator, and multi-copper oxidase domains, which are found in both Gram-positive (like Mycobacterium tuberculosis and Enterococcus hirae) and Gram-negative bacteria (like E. coli and Pseudomonas aeruginosa). The existence of 4 multi-copper oxidase genes, which supplied an additional copper defense mechanism, could be intriguing information regarding Gram-positive bacteria such as Arthrobacter sp. PAMC25284. In addition, our strain PAMC25284 has the same MmcO gene as M. tuberculosis, with a locus tag KY499_RS04055 similarity of 40.61%, which is the highest among the Gram-positive and Gram-negative bacteria studied for this gene. Our cold-adapted Arthrobacter sp. strain PAMC25564 was published previously but did not contain a multi-copper oxidase domain-containing gene, but strain PAMC25284 was studied in this study. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic International Journal of Microbiology 2022 1 12 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Microbiology QR1-502 |
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Microbiology QR1-502 Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
topic_facet |
Microbiology QR1-502 |
description |
The genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified a species of Arthrobacter isolated from seawater in the polar region using 16S rRNA sequence analysis. The strain PAMC25284 genome consists of a circular chromosome with a GC content of 65.6% and is projected to contain 3,588 genes, of which 3,150 are protein coding, 366 are pseudogenes, 19 are rRNA coding, and 50 are tRNA coding genes. Using comparative genomics, we showed that PMAC25284 has copper-transporting ATPases, copper chaperone, copper-responsive transcriptional regulator, and multi-copper oxidase domains, which are found in both Gram-positive (like Mycobacterium tuberculosis and Enterococcus hirae) and Gram-negative bacteria (like E. coli and Pseudomonas aeruginosa). The existence of 4 multi-copper oxidase genes, which supplied an additional copper defense mechanism, could be intriguing information regarding Gram-positive bacteria such as Arthrobacter sp. PAMC25284. In addition, our strain PAMC25284 has the same MmcO gene as M. tuberculosis, with a locus tag KY499_RS04055 similarity of 40.61%, which is the highest among the Gram-positive and Gram-negative bacteria studied for this gene. Our cold-adapted Arthrobacter sp. strain PAMC25564 was published previously but did not contain a multi-copper oxidase domain-containing gene, but strain PAMC25284 was studied in this study. |
format |
Article in Journal/Newspaper |
author |
Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh |
author_facet |
Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh |
author_sort |
Jayram Karmacharya |
title |
Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
title_short |
Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
title_full |
Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
title_fullStr |
Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
title_full_unstemmed |
Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
title_sort |
complete genome sequencing of polar arthrobacter sp. pamc25284, copper tolerance potential unraveled with genomic analysis |
publisher |
Hindawi Limited |
publishDate |
2022 |
url |
https://doi.org/10.1155/2022/1162938 https://doaj.org/article/7f9cc35f56214c8fba9da79ed3dca1a9 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Microbiology, Vol 2022 (2022) |
op_relation |
http://dx.doi.org/10.1155/2022/1162938 https://doaj.org/toc/1687-9198 1687-9198 doi:10.1155/2022/1162938 https://doaj.org/article/7f9cc35f56214c8fba9da79ed3dca1a9 |
op_doi |
https://doi.org/10.1155/2022/1162938 |
container_title |
International Journal of Microbiology |
container_volume |
2022 |
container_start_page |
1 |
op_container_end_page |
12 |
_version_ |
1766016340104052736 |