In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts
Objective: To investigate in vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.). Methods: Total phenolic and flavonoid contents of the Korean blueberry water and ethanol extracts were determined before determining the potential of the extracts as antioxi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2014
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Online Access: | https://doi.org/10.12980/APJTB.4.2014C1008 https://doaj.org/article/7f6613ea5e79421ba723e4b9262afe5f |
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ftdoajarticles:oai:doaj.org/article:7f6613ea5e79421ba723e4b9262afe5f 2023-05-15T15:10:36+02:00 In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts Nadira Binte Samad Trishna Debnath Michael Ye Md. Abul Hasnat Beong Ou Lim 2014-10-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1008 https://doaj.org/article/7f6613ea5e79421ba723e4b9262afe5f EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300216 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1008 https://doaj.org/article/7f6613ea5e79421ba723e4b9262afe5f Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 10, Pp 807-815 (2014) Phenolics Flavonoids DPPH radical scavenging Nitrite scavenging DNA damage Anti–inflammatory activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1008 2022-12-30T21:53:03Z Objective: To investigate in vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.). Methods: Total phenolic and flavonoid contents of the Korean blueberry water and ethanol extracts were determined before determining the potential of the extracts as antioxidant. Antioxidant activity of the extracts was determined by following some well established methods for free radical scavenging such as 2,2-diphenyl-picrylhydrazyl hydrate, 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid), free radical induced DNA damage, superoxide dismutase-like and catalase assay etc. Furthermore, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan and nitric oxide assay were performed to determine the anti-inflammatory activity of the extracts. Results: Total phenolic contents were found (115.0±3.0) and (4.2±3.0) mg GAE/100 g fresh mass for both extracts, respectively and flavonoid contents were (1 942.8±7.0) and (1 292.1±6.0) mg CE/100g fresh mass for water and ethonal extracts, respectively. Both the extracts displayed significant scavenging activity of some radicals such as 2,2-diphenyl-picrylhydrazyl hydrate (IC50 at 1.8 mg/mL and 2.05 mg/mL, respectively), 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (IC50 at 1.5 mg/mL and 1.6 mg/mL, respectively) and nitrite (IC50 at 1.7 mg/mL and 1.5 mg/mL, respectively) etc. The extracts were found to prevent inflammation as well by reducing nitric oxide production and cytotoxicity in cell. Conclusions: The findings suggest that the fresh Korean blueberry could be used as a source of natural antioxidants and anti-inflammatory agents. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 10 807 815 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Phenolics Flavonoids DPPH radical scavenging Nitrite scavenging DNA damage Anti–inflammatory activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Phenolics Flavonoids DPPH radical scavenging Nitrite scavenging DNA damage Anti–inflammatory activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nadira Binte Samad Trishna Debnath Michael Ye Md. Abul Hasnat Beong Ou Lim In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts |
topic_facet |
Phenolics Flavonoids DPPH radical scavenging Nitrite scavenging DNA damage Anti–inflammatory activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate in vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.). Methods: Total phenolic and flavonoid contents of the Korean blueberry water and ethanol extracts were determined before determining the potential of the extracts as antioxidant. Antioxidant activity of the extracts was determined by following some well established methods for free radical scavenging such as 2,2-diphenyl-picrylhydrazyl hydrate, 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid), free radical induced DNA damage, superoxide dismutase-like and catalase assay etc. Furthermore, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan and nitric oxide assay were performed to determine the anti-inflammatory activity of the extracts. Results: Total phenolic contents were found (115.0±3.0) and (4.2±3.0) mg GAE/100 g fresh mass for both extracts, respectively and flavonoid contents were (1 942.8±7.0) and (1 292.1±6.0) mg CE/100g fresh mass for water and ethonal extracts, respectively. Both the extracts displayed significant scavenging activity of some radicals such as 2,2-diphenyl-picrylhydrazyl hydrate (IC50 at 1.8 mg/mL and 2.05 mg/mL, respectively), 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (IC50 at 1.5 mg/mL and 1.6 mg/mL, respectively) and nitrite (IC50 at 1.7 mg/mL and 1.5 mg/mL, respectively) etc. The extracts were found to prevent inflammation as well by reducing nitric oxide production and cytotoxicity in cell. Conclusions: The findings suggest that the fresh Korean blueberry could be used as a source of natural antioxidants and anti-inflammatory agents. |
format |
Article in Journal/Newspaper |
author |
Nadira Binte Samad Trishna Debnath Michael Ye Md. Abul Hasnat Beong Ou Lim |
author_facet |
Nadira Binte Samad Trishna Debnath Michael Ye Md. Abul Hasnat Beong Ou Lim |
author_sort |
Nadira Binte Samad |
title |
In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts |
title_short |
In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts |
title_full |
In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts |
title_fullStr |
In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts |
title_full_unstemmed |
In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts |
title_sort |
in vitro antioxidant and anti–inflammatory activities of korean blueberry (vaccinium corymbosum l.) extracts |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1008 https://doaj.org/article/7f6613ea5e79421ba723e4b9262afe5f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 10, Pp 807-815 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300216 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1008 https://doaj.org/article/7f6613ea5e79421ba723e4b9262afe5f |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1008 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
10 |
container_start_page |
807 |
op_container_end_page |
815 |
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1766341596989620224 |