An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus)
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus) . The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed agai...
Published in: | Acta Veterinaria Scandinavica |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2002
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1751-0147-43-211 https://doaj.org/article/7f63b0a6dc6c4ed6b37609ca2bfc152e |
id |
ftdoajarticles:oai:doaj.org/article:7f63b0a6dc6c4ed6b37609ca2bfc152e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:7f63b0a6dc6c4ed6b37609ca2bfc152e 2023-05-15T13:19:46+02:00 An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) Åkerstedt J 2002-12-01T00:00:00Z https://doi.org/10.1186/1751-0147-43-211 https://doaj.org/article/7f63b0a6dc6c4ed6b37609ca2bfc152e EN eng BMC http://www.actavetscand.com/content/43/4/211 https://doaj.org/toc/1751-0147 doi:10.1186/1751-0147-43-211 1751-0147 https://doaj.org/article/7f63b0a6dc6c4ed6b37609ca2bfc152e Acta Veterinaria Scandinavica, Vol 43, Iss 4, Pp 211-220 (2002) microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence Veterinary medicine SF600-1100 article 2002 ftdoajarticles https://doi.org/10.1186/1751-0147-43-211 2022-12-31T09:09:09Z Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus) . The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99), and the ELISA and IFAT (κ = 0.958). There was no significant statistical difference between the tests (p > 0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. Article in Journal/Newspaper Alopex lagopus Directory of Open Access Journals: DOAJ Articles Acta Veterinaria Scandinavica 43 4 211 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence Veterinary medicine SF600-1100 |
spellingShingle |
microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence Veterinary medicine SF600-1100 Åkerstedt J An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
topic_facet |
microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence Veterinary medicine SF600-1100 |
description |
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus) . The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99), and the ELISA and IFAT (κ = 0.958). There was no significant statistical difference between the tests (p > 0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. |
format |
Article in Journal/Newspaper |
author |
Åkerstedt J |
author_facet |
Åkerstedt J |
author_sort |
Åkerstedt J |
title |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_short |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_full |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_fullStr |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_full_unstemmed |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_sort |
indirect elisa for detection of encephalitozoon cuniculi infection in farmed blue foxes (alopex lagopus) |
publisher |
BMC |
publishDate |
2002 |
url |
https://doi.org/10.1186/1751-0147-43-211 https://doaj.org/article/7f63b0a6dc6c4ed6b37609ca2bfc152e |
genre |
Alopex lagopus |
genre_facet |
Alopex lagopus |
op_source |
Acta Veterinaria Scandinavica, Vol 43, Iss 4, Pp 211-220 (2002) |
op_relation |
http://www.actavetscand.com/content/43/4/211 https://doaj.org/toc/1751-0147 doi:10.1186/1751-0147-43-211 1751-0147 https://doaj.org/article/7f63b0a6dc6c4ed6b37609ca2bfc152e |
op_doi |
https://doi.org/10.1186/1751-0147-43-211 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
43 |
container_issue |
4 |
container_start_page |
211 |
_version_ |
1766349424293838848 |