Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia
Borrelia burgdorferi sensu lato (s.l.) DNA was detected by PCR in Ixodes persulcatus Schulze, 1930, Haemaphysalis concinna Koch, 1844, Haemaphysalis japonica douglasi Nuttall et Warburton, 1915 and Dermacentor silvarum Olenev, 1932 ticks collected in the Amur region, the Jewish Autonomous region, th...
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ftdoajarticles:oai:doaj.org/article:7f30b39199dd45839276a80838cda687 2023-05-15T18:09:05+02:00 Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia Natalia M. Pukhovskaya Olga V. Morozova Nelya P. Vysochina Nadejda B. Belozerova Leonid I. Ivanov 2019-04-01T00:00:00Z https://doi.org/10.1016/j.ijppaw.2019.01.005 https://doaj.org/article/7f30b39199dd45839276a80838cda687 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213224419300197 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2019.01.005 https://doaj.org/article/7f30b39199dd45839276a80838cda687 International Journal for Parasitology: Parasites and Wildlife, Vol 8, Iss , Pp 192-202 (2019) Zoology QL1-991 article 2019 ftdoajarticles https://doi.org/10.1016/j.ijppaw.2019.01.005 2022-12-31T11:58:57Z Borrelia burgdorferi sensu lato (s.l.) DNA was detected by PCR in Ixodes persulcatus Schulze, 1930, Haemaphysalis concinna Koch, 1844, Haemaphysalis japonica douglasi Nuttall et Warburton, 1915 and Dermacentor silvarum Olenev, 1932 ticks collected in the Amur region, the Jewish Autonomous region, the Sakhalin region and on the Khabarovsk territory. Infection rate of I. persulcatus with B. burgdorferi s.l. 10–69% exceeded the corresponding values of three other tick species in all examined regions during 1999–2014 despite different tick abundance and dominance structure. Bacterial loads estimated on the base of quantitative real time PCR varied from 102 to 109 genome-equivalents per a tick with maximal values for I. persulcatus and H. japonica. Phylogenetic analysis of 16S rRNA gene and 5S23S rRNA intergenic spacer nucleotide sequences revealed two species: 1) Borrelia garinii of Asian type NT29 with several isolates of European type 20047; 2) Borrelia afzelii with identical sequences of the majority of studied isolates and VS461 reference strain in all regions except the Sakhalin Island where B. afzelii was not found. Borrelia miyamotoi of the relapsing fever group was detected as monoinfection or in combination with B. burgdorferi s.l. in 4.0 ± 0.9% and 4.8 ± 0.9% I. persulcatus ticks, respectively. Multiple locus sequence analysis of three fragments of 16S rRNA, glpQ and p66 genes proved that all the Far Eastern B. miyamotoi isolates belonged to the Asian type identical to FR64b strain (GenBank CP004217) from Japan. Wide distribution of Borrelia DNA in ticks, relative genetic homogeneity with similar sequences of the coding regions and the intergenic spacer of Borrelia wild isolates and temporal stability with high homology levels of the Far Eastern isolates of B. garinii, B. afzelii and B. miyamotoi with previously described spirochetes from the surrounding regions of Russia, China and Japan allowed us to suggest multiple ecological niches as the stability factor of the parasitic system. Keywords: Ixodid ... Article in Journal/Newspaper Sakhalin Directory of Open Access Journals: DOAJ Articles International Journal for Parasitology: Parasites and Wildlife 8 192 202 |
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Directory of Open Access Journals: DOAJ Articles |
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Zoology QL1-991 |
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Zoology QL1-991 Natalia M. Pukhovskaya Olga V. Morozova Nelya P. Vysochina Nadejda B. Belozerova Leonid I. Ivanov Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia |
topic_facet |
Zoology QL1-991 |
description |
Borrelia burgdorferi sensu lato (s.l.) DNA was detected by PCR in Ixodes persulcatus Schulze, 1930, Haemaphysalis concinna Koch, 1844, Haemaphysalis japonica douglasi Nuttall et Warburton, 1915 and Dermacentor silvarum Olenev, 1932 ticks collected in the Amur region, the Jewish Autonomous region, the Sakhalin region and on the Khabarovsk territory. Infection rate of I. persulcatus with B. burgdorferi s.l. 10–69% exceeded the corresponding values of three other tick species in all examined regions during 1999–2014 despite different tick abundance and dominance structure. Bacterial loads estimated on the base of quantitative real time PCR varied from 102 to 109 genome-equivalents per a tick with maximal values for I. persulcatus and H. japonica. Phylogenetic analysis of 16S rRNA gene and 5S23S rRNA intergenic spacer nucleotide sequences revealed two species: 1) Borrelia garinii of Asian type NT29 with several isolates of European type 20047; 2) Borrelia afzelii with identical sequences of the majority of studied isolates and VS461 reference strain in all regions except the Sakhalin Island where B. afzelii was not found. Borrelia miyamotoi of the relapsing fever group was detected as monoinfection or in combination with B. burgdorferi s.l. in 4.0 ± 0.9% and 4.8 ± 0.9% I. persulcatus ticks, respectively. Multiple locus sequence analysis of three fragments of 16S rRNA, glpQ and p66 genes proved that all the Far Eastern B. miyamotoi isolates belonged to the Asian type identical to FR64b strain (GenBank CP004217) from Japan. Wide distribution of Borrelia DNA in ticks, relative genetic homogeneity with similar sequences of the coding regions and the intergenic spacer of Borrelia wild isolates and temporal stability with high homology levels of the Far Eastern isolates of B. garinii, B. afzelii and B. miyamotoi with previously described spirochetes from the surrounding regions of Russia, China and Japan allowed us to suggest multiple ecological niches as the stability factor of the parasitic system. Keywords: Ixodid ... |
format |
Article in Journal/Newspaper |
author |
Natalia M. Pukhovskaya Olga V. Morozova Nelya P. Vysochina Nadejda B. Belozerova Leonid I. Ivanov |
author_facet |
Natalia M. Pukhovskaya Olga V. Morozova Nelya P. Vysochina Nadejda B. Belozerova Leonid I. Ivanov |
author_sort |
Natalia M. Pukhovskaya |
title |
Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia |
title_short |
Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia |
title_full |
Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia |
title_fullStr |
Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia |
title_full_unstemmed |
Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia |
title_sort |
prevalence of borrelia burgdorferi sensu lato and borrelia miyamotoi in ixodid ticks in the far east of russia |
publisher |
Elsevier |
publishDate |
2019 |
url |
https://doi.org/10.1016/j.ijppaw.2019.01.005 https://doaj.org/article/7f30b39199dd45839276a80838cda687 |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
International Journal for Parasitology: Parasites and Wildlife, Vol 8, Iss , Pp 192-202 (2019) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213224419300197 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2019.01.005 https://doaj.org/article/7f30b39199dd45839276a80838cda687 |
op_doi |
https://doi.org/10.1016/j.ijppaw.2019.01.005 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
8 |
container_start_page |
192 |
op_container_end_page |
202 |
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1766181505703346176 |