2nd International external quality control assessment for the molecular diagnosis of dengue infections.
BACKGROUND: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical and sub-tropical countries worldwide. International air travel is facilitating rapid global movement of DENV, increasing the risk of severe dengue epidemics by introducing different...
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ftdoajarticles:oai:doaj.org/article:7ef34b1b4e124e30b5020805d2e923a9 2023-05-15T15:16:32+02:00 2nd International external quality control assessment for the molecular diagnosis of dengue infections. Cristina Domingo Matthias Niedrig Anette Teichmann Marco Kaiser Leonid Rumer Richard G Jarman Oliver Donoso-Mantke 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000833 https://doaj.org/article/7ef34b1b4e124e30b5020805d2e923a9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2950135?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000833 https://doaj.org/article/7ef34b1b4e124e30b5020805d2e923a9 PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e1000938 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000833 2022-12-31T11:17:08Z BACKGROUND: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical and sub-tropical countries worldwide. International air travel is facilitating rapid global movement of DENV, increasing the risk of severe dengue epidemics by introducing different serotypes. Accurate diagnosis is critical for early initiation of preventive measures. Different reverse transcriptase PCR (RT-PCR) methods are available, which should be evaluated and standardized. Epidemiological and laboratory-based surveillance is required to monitor and guide dengue prevention and control programmes, i.e., by mosquito control or possible vaccination (as soon as an effective and safe vaccine becomes available). OBJECTIVE: The purpose of the external quality assurance (EQA) study described is to assess the efficiency and accuracy of dengue molecular diagnosis methods applied by expert laboratories. STUDY DESIGN: A panel of 12 human plasma samples was distributed and tested for DENV-specific RNA. The panel comprised 9 samples spiked with different DENV serotypes (DENV-1 to DENV-4), including 10-fold dilution series of DENV-1 and DENV-3. Two specificity controls consisted of a sample with a pool of 4 other flaviviruses and a sample with chikungunya virus. A negative control sample was also included. RESULTS: Thirty-seven laboratories (from Europe, Middle East Asia, Asia, the Americas/Caribbean, and Africa) participated in this EQA study, and reports including 46 sets of results were returned. Performance among laboratories varied according to methodologies used. Only 5 (10.9%) data sets met all criteria with optimal performance, and 4 (8.7%) with acceptable performance, while 37 (80.4%) reported results showed the need for improvement regarding accomplishment of dengue molecular diagnosis. Failures were mainly due to lack of sensitivity and the presence of false positives. CONCLUSIONS: The EQA provides information on each laboratory's efficacy of RT-PCR techniques for dengue diagnosis and indicates ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 10 e833 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cristina Domingo Matthias Niedrig Anette Teichmann Marco Kaiser Leonid Rumer Richard G Jarman Oliver Donoso-Mantke 2nd International external quality control assessment for the molecular diagnosis of dengue infections. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical and sub-tropical countries worldwide. International air travel is facilitating rapid global movement of DENV, increasing the risk of severe dengue epidemics by introducing different serotypes. Accurate diagnosis is critical for early initiation of preventive measures. Different reverse transcriptase PCR (RT-PCR) methods are available, which should be evaluated and standardized. Epidemiological and laboratory-based surveillance is required to monitor and guide dengue prevention and control programmes, i.e., by mosquito control or possible vaccination (as soon as an effective and safe vaccine becomes available). OBJECTIVE: The purpose of the external quality assurance (EQA) study described is to assess the efficiency and accuracy of dengue molecular diagnosis methods applied by expert laboratories. STUDY DESIGN: A panel of 12 human plasma samples was distributed and tested for DENV-specific RNA. The panel comprised 9 samples spiked with different DENV serotypes (DENV-1 to DENV-4), including 10-fold dilution series of DENV-1 and DENV-3. Two specificity controls consisted of a sample with a pool of 4 other flaviviruses and a sample with chikungunya virus. A negative control sample was also included. RESULTS: Thirty-seven laboratories (from Europe, Middle East Asia, Asia, the Americas/Caribbean, and Africa) participated in this EQA study, and reports including 46 sets of results were returned. Performance among laboratories varied according to methodologies used. Only 5 (10.9%) data sets met all criteria with optimal performance, and 4 (8.7%) with acceptable performance, while 37 (80.4%) reported results showed the need for improvement regarding accomplishment of dengue molecular diagnosis. Failures were mainly due to lack of sensitivity and the presence of false positives. CONCLUSIONS: The EQA provides information on each laboratory's efficacy of RT-PCR techniques for dengue diagnosis and indicates ... |
format |
Article in Journal/Newspaper |
author |
Cristina Domingo Matthias Niedrig Anette Teichmann Marco Kaiser Leonid Rumer Richard G Jarman Oliver Donoso-Mantke |
author_facet |
Cristina Domingo Matthias Niedrig Anette Teichmann Marco Kaiser Leonid Rumer Richard G Jarman Oliver Donoso-Mantke |
author_sort |
Cristina Domingo |
title |
2nd International external quality control assessment for the molecular diagnosis of dengue infections. |
title_short |
2nd International external quality control assessment for the molecular diagnosis of dengue infections. |
title_full |
2nd International external quality control assessment for the molecular diagnosis of dengue infections. |
title_fullStr |
2nd International external quality control assessment for the molecular diagnosis of dengue infections. |
title_full_unstemmed |
2nd International external quality control assessment for the molecular diagnosis of dengue infections. |
title_sort |
2nd international external quality control assessment for the molecular diagnosis of dengue infections. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000833 https://doaj.org/article/7ef34b1b4e124e30b5020805d2e923a9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e1000938 (2010) |
op_relation |
http://europepmc.org/articles/PMC2950135?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000833 https://doaj.org/article/7ef34b1b4e124e30b5020805d2e923a9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000833 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
10 |
container_start_page |
e833 |
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1766346831652978688 |